ShEEP Request for Keyence BZ-X800E All-in-One Fluorescence Microscope
ShEEP 请求购买 Keyence BZ-X800E 一体式荧光显微镜
基本信息
- 批准号:9907801
- 负责人:
- 金额:--
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2019
- 资助国家:美国
- 起止时间:2019-09-01 至 2020-09-30
- 项目状态:已结题
- 来源:
- 关键词:Activities of Daily LivingAcuteAge related macular degenerationAgingAnimalsAntioxidantsBiological MarkersCarbon DioxideCardiacCatalytic RNACell DeathCell physiologyCellsChronicClinicCollaborationsCollectionColorComputer softwareControlled EnvironmentCultured CellsDataDevelopmentDevicesDimensionsDiseaseEquipmentFluorescenceFunctional disorderFundingFutureGenerationsGliosisHealthHeart ArrestHematopoieticHistologicHumanIdiopathic Parkinson DiseaseImageImage AnalysisImmersion Investigative TechniqueInterval trainingInterventionKineticsManufacturer NameMeasurementMediatingMethodologyMicroscopeMidbrain structureModelingMonitorMorphologyMusMuscle CellsMuscle FibersMusculoskeletalMutationMyocardial InfarctionOilsOrphanOsteoclastsOutcomeOxidative StressParkinson DiseasePathologicPathologic ProcessesPhasePhysiologicalPopulationProcessQuality of lifeRNARNA InterferenceReagentResearchResearch PersonnelResolutionRetinal DegenerationRetinitis PigmentosaRoleSeveritiesSheepSignal TransductionSkeletal MuscleSkeletal boneSlideStem cellsTemperatureTherapeuticTherapeutic AgentsTherapeutic InterventionThree-Dimensional ImageThree-Dimensional ImagingTimeTissuesTranslatingTranslational ResearchVeteransVisualWorkadult stem cellbasebench to bedsidebonebone resorbing activitycardiac repairdietary supplementsdopaminergic neuroneffective interventionexosomeflasksfluorescence imagingfluorescence microscopefluorophorefrailtyimprovedinduced pluripotent stem cellinsightinstrumentlive cell imagingmacrophagemovienovelparkin gene/proteinpreventreal-time imagesrepairedstemtherapeutic candidatetooltrafficking
项目摘要
Project Abstract
The Keyence BZ-X800E All-in-One fluorescent microscope is a unique instrument that
provides multi-color fluorescent imaging, 3D image rendering and measurement localization, live cell
and time-lapse video generation, automated multi-image collection, and real-time image stitching and
image analysis. Thus, the Keyence microscope will be a crucial instrument for VAWNYHS
investigators, and the unique features will have immediate benefits for VA-funded projects below.
Dr. Troen’s work investigates musculoskeletal aging, frailty, and functional capacity in older
veterans. Dr. Troen is conducting both human and animal studies to explore the benefits of high
intensity interval training (HIIT) and nutritional supplements on skeletal muscle and bone morphology
and function during aging. The Keyence will allow more precise assessments of muscle fibers in a
fraction of the time of the current methodology. The Keyence will also be able to generate time-lapse
live cell imaging video to observe the formation and activity of bone resorbing osteoclasts, capture the
impacts of reagent interventions, and facilitate analysis of bones from experimental animals.
Dr. Fliesler’s projects investigate the underlying mechanism of progressive retinal degeneration
and visual dysfunction associated with blast overpressure-induced polytrauma, and the impact of
novel antioxidants as therapeutic agents to prevent, minimize, or slow the progression of the
pathological processes. The Keyence microscope will afford quantitative assessment of histological
damage and biomarkers of oxidative stress, cell death, and gliosis.
Dr. Sullivan’s study investigates the development of post-transcriptional gene silencing agents
such as ribozymes (catalytic RNAs) as candidate therapeutics for orphan retinal degenerations (e.g.
retinitis pigmentosa) and common age-related macular degeneration (AMD). The Keyence will
provide dramatic new methodologies to observe cellular RNA trafficking and investigate the kinetics
and steady-state outcomes of catalytic RNA (ribozyme) therapeutics.
Dr. Canty’s study investigates dynamic remodeling during sudden cardiac arrest and cardiac
stem cell mediated repair. The Keyence will facilitate characterization of specific hematopoietic
subtypes and macrophage polarization and enable characterization of stems in culture to better
understand how adult stem cells stimulate endogenous myocyte proliferation.
Dr. Feng’s study seeks to understand how mutations of parkin cause the selective degeneration
of human nigral dopaminergic neurons and the ensuing Parkinson’s disease. The Keyence will allow
the ability to monitor morphological changes and enable imaging of iPSC-derived midbrain DA
neurons, to identify impacts of parkin mutations as a model of idiopathic Parkinson’s disease.
Dr. Lang’s research seeks to determine the functional role of exosomes in stem cell-mediated
cardiac repair. The Keyence microscope will enhance this work by facilitating extremely high
resolution immunohistological analysis of murine cardiac tissue following myocardial infarction and
evaluating the potential of exosome-based therapy to ameliorate damaged tissues.
The range of applications provided by the Keyence BZ-X800E microscope will greatly facilitate
our understanding of the underlying acute and chronic processes that result in physiological
dysfunction, disease, and health decline. Further, it will be an essential tool for the VAWNYHS
researchers to develop effective interventions to minimize the severity, delay the onset, or arrest the
progression of frailty and ultimately improve the quality of life in the veteran population.
项目摘要
Keyence BZ-X800E 一体式荧光显微镜是一款独特的仪器,
提供多色荧光成像、3D 图像渲染和测量定位、活细胞
和延时视频生成、自动多图像采集以及实时图像拼接和
图像分析。因此,Keyence 显微镜将成为 VAWNYHS 的重要仪器
研究人员认为,这些独特的功能将为以下 VA 资助的项目带来直接好处。
Troen 博士的工作研究了老年人的肌肉骨骼衰老、虚弱和功能能力
退伍军人。 Troen 博士正在进行人体和动物研究,以探索高浓度的好处
强度间歇训练(HIIT)和营养补充剂对骨骼肌和骨骼形态的影响
和衰老过程中的功能。 Keyence 将能够更精确地评估肌肉纤维
当前方法的时间的一小部分。 Keyence 还能够生成延时
活细胞成像视频观察骨吸收破骨细胞的形成和活性,捕捉
试剂干预的影响,并促进实验动物骨骼的分析。
Fliesler 博士的项目研究了进行性视网膜变性的潜在机制
和与爆炸超压引起的多发伤相关的视觉功能障碍,以及
新型抗氧化剂作为治疗剂来预防、最小化或减缓疾病的进展
病理过程。 Keyence 显微镜将提供组织学的定量评估
氧化应激、细胞死亡和神经胶质增生的损伤和生物标志物。
沙利文博士的研究调查了转录后基因沉默剂的开发
例如核酶(催化 RNA)作为孤儿视网膜变性(例如视网膜变性)的候选疗法。
视网膜色素变性)和常见的年龄相关性黄斑变性(AMD)。基恩士将
提供引人注目的新方法来观察细胞 RNA 运输并研究动力学
以及催化 RNA(核酶)疗法的稳态结果。
Canty 博士的研究调查了心脏骤停和心脏骤停期间的动态重塑。
干细胞介导的修复。 Keyence 将促进特定造血细胞的表征
亚型和巨噬细胞极化,并能够更好地表征培养物中的茎
了解成体干细胞如何刺激内源性肌细胞增殖。
冯博士的研究旨在了解parkin突变如何导致选择性变性
人类黑质多巴胺能神经元和随后的帕金森病。 Keyence 将允许
能够监测形态变化并实现 iPSC 衍生的中脑 DA 成像
神经元,以确定帕金突变作为特发性帕金森病模型的影响。
Lang 博士的研究旨在确定外泌体在干细胞介导的功能中的功能作用
心脏修复。基恩士显微镜将通过促进极高的
心肌梗死后小鼠心脏组织的分辨率免疫组织学分析
评估基于外泌体的疗法改善受损组织的潜力。
Keyence BZ-X800E 显微镜提供的应用范围将极大地方便
我们对导致生理现象的潜在急性和慢性过程的理解
功能障碍、疾病和健康状况下降。此外,它将成为 VAWNYHS 的重要工具
研究人员制定有效的干预措施,以尽量减少严重程度、推迟发病或阻止病情的发展
衰弱的进展并最终改善退伍军人群体的生活质量。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Bruce R. Troen其他文献
Effects of a 6-Week Treadmill Training With and Without Virtual Reality on Frailty in People With Multiple Sclerosis
为期6周有虚拟现实和无虚拟现实的跑步机训练对多发性硬化症患者虚弱状况的影响
- DOI:
10.1016/j.apmr.2024.09.010 - 发表时间:
2025-02-01 - 期刊:
- 影响因子:3.700
- 作者:
Tobia Zanotto;Irina Galperin;Danya Pradeep Kumar;Anat Mirelman;Shahar Yehezkyahu;Keren Regev;Arnon Karni;Tanja Schmitz-Hübsch;Friedemann Paul;Sharon G. Lynch;Abiodun E. Akinwuntan;Jianghua He;Bruce R. Troen;Hannes Devos;Jeffrey M. Hausdorff;Jacob J. Sosnoff - 通讯作者:
Jacob J. Sosnoff
Comparative Responsiveness of HL-60, HL-60R, and HL-60R<sup>+</sup> (LRARSN) Cells to Retinoic Acid, Calcitriol, 9 <em>cis</em>-Retinoic Acid, and Sodium Butyrate
- DOI:
10.1182/blood.v86.7.2475.2475 - 发表时间:
1995-10-01 - 期刊:
- 影响因子:
- 作者:
Kevin B. Atkins;Bruce R. Troen - 通讯作者:
Bruce R. Troen
Cloning and expression of the gene for the major excreted protein of transformed mouse fibroblasts. A secreted lysosomal protease regulated by transformation.
转化小鼠成纤维细胞主要分泌蛋白基因的克隆和表达。
- DOI:
10.1016/s0021-9258(19)57386-0 - 发表时间:
1988 - 期刊:
- 影响因子:0
- 作者:
Bruce R. Troen;D. Ascherman;Daphne Atlas;Michael M. Gottesman - 通讯作者:
Michael M. Gottesman
Resveratrol: Effects on Lipids and Cardiovascular Risk
白藜芦醇:对血脂和心血管风险的影响
- DOI:
10.1007/s12170-012-0289-2 - 发表时间:
2013 - 期刊:
- 影响因子:1.9
- 作者:
E. Cherniack;E. Cherniack;Bruce R. Troen;Bruce R. Troen - 通讯作者:
Bruce R. Troen
Bruce R. Troen的其他文献
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{{ truncateString('Bruce R. Troen', 18)}}的其他基金
Promoting cognitive resilience and reducing frailty in older Veterans with bright light therapy
通过强光疗法提高老年退伍军人的认知能力并减少虚弱
- 批准号:
10590503 - 财政年份:2023
- 资助金额:
-- - 项目类别:
High Intensity Interval Training (HIIT) to Reduce Frailty and Enhance Resilience in Older Veterans
高强度间歇训练(HIIT)可减少老年退伍军人的虚弱并增强其恢复能力
- 批准号:
10539160 - 财政年份:2023
- 资助金额:
-- - 项目类别:
The impacts of vitamin D status and HIIT on physical performance and frailty during aging
维生素 D 状态和 HIIT 对衰老过程中身体机能和虚弱的影响
- 批准号:
10229660 - 财政年份:2020
- 资助金额:
-- - 项目类别:
ShEEP Request for Intracellular and Extracellular Protein Signaling Station (IEPSS)
ShEEP 请求细胞内和细胞外蛋白质信号转导站 (IEPSS)
- 批准号:
10178936 - 财政年份:2020
- 资助金额:
-- - 项目类别:
Enhancing Geroscience Scholarship and Faculty Development to Minimize Frailty and Maximize Healthspan
加强老年科学奖学金和教师发展,以最大限度地减少虚弱和最大限度地延长健康寿命
- 批准号:
10349503 - 财政年份:2019
- 资助金额:
-- - 项目类别:
Enhancing Geroscience Scholarship and Faculty Development to Minimize Frailty and Maximize Healthspan
加强老年科学奖学金和教师发展,以最大限度地减少虚弱和最大限度地延长健康寿命
- 批准号:
9927970 - 财政年份:2019
- 资助金额:
-- - 项目类别:
High intensity interval training and nicotinamide riboside treatment to enhance functional capacity and reduce frailty during aging
高强度间歇训练和烟酰胺核苷治疗可增强功能能力并减少衰老过程中的虚弱
- 批准号:
10663245 - 财政年份:2019
- 资助金额:
-- - 项目类别:
Enhancing Geroscience Scholarship and Faculty Development to Minimize Frailty and Maximize Healthspan
加强老年科学奖学金和教师发展,以最大限度地减少虚弱和最大限度地延长健康寿命
- 批准号:
10581712 - 财政年份:2019
- 资助金额:
-- - 项目类别:
High intensity interval training and nicotinamide riboside treatment to enhance functional capacity and reduce frailty during aging
高强度间歇训练和烟酰胺核苷治疗可增强功能能力并减少衰老过程中的虚弱
- 批准号:
10266089 - 财政年份:2019
- 资助金额:
-- - 项目类别:
High intensity interval training and nicotinamide riboside treatment to enhance functional capacity and reduce frailty during aging
高强度间歇训练和烟酰胺核苷治疗可增强功能能力并减少衰老过程中的虚弱
- 批准号:
9974279 - 财政年份:2019
- 资助金额:
-- - 项目类别:
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