ShEEP Request for Keyence BZ-X800E All-in-One Fluorescence Microscope

项目摘要 Keyence BZ-X800 E多功能一体荧光显微镜是一款独特的仪器， 提供多色荧光成像、3D图像渲染和测量定位、活细胞 和延时视频生成，自动多图像采集，实时图像拼接， 图像分析因此，Keyence显微镜将成为VAWNYHS的关键仪器 这些独特的功能将对以下VA资助的项目产生直接效益。 Troen博士的工作是研究老年人的肌肉骨骼老化、脆弱和功能能力。 老兵Troen博士正在进行人类和动物研究，以探索高血糖的益处。 强度间歇训练（HIIT）和营养补充剂对骨骼肌和骨形态的影响 并在衰老过程中发挥作用。Keyence将允许更精确地评估肌肉纤维， 目前方法的一小部分时间。Keyence还将能够生成延时 活细胞成像视频，观察骨吸收破骨细胞的形成和活性，捕捉 试剂干预的影响，并促进对实验动物骨骼的分析。 Fliesler博士的项目研究了进行性视网膜变性的潜在机制 和视觉功能障碍与冲击波超压引起的多发伤， 作为治疗剂的新的抗氧化剂，以预防、最小化或减缓 病理过程。Keyence显微镜将提供组织学定量评估 氧化应激、细胞死亡和神经胶质增生的损伤和生物标志物。 沙利文博士的研究调查了转录后基因沉默剂的发展 例如核酶（催化RNA）作为孤儿视网膜变性的候选治疗剂（例如， 色素性视网膜炎）和常见的年龄相关性黄斑变性（AMD）。Keyence将 提供了引人注目的新方法来观察细胞RNA运输和研究动力学 和催化RNA（核酶）疗法的稳态结果。 Canty博士的研究调查了心脏骤停和心脏骤停期间的动态重塑。 干细胞介导修复Keyence将有助于表征特定的造血干细胞， 亚型和巨噬细胞极化，并使培养物中的茎的表征，以更好地 了解成体干细胞如何刺激内源性肌细胞增殖。 冯博士的研究试图了解帕金基因的突变是如何导致选择性变性的。 黑质多巴胺能神经元和随之而来的帕金森病。基恩士号将允许 监测形态学变化并使iPSC衍生的中脑DA成像的能力 神经元，以确定parkin突变作为特发性帕金森病模型的影响。 Lang博士的研究旨在确定外泌体在干细胞介导的细胞凋亡中的功能作用。 心脏修复Keyence显微镜将通过促进极高的 心肌梗死后小鼠心脏组织的分辨率免疫组织学分析， 评估基于外泌体的治疗改善受损组织的潜力。 Keyence BZ-X800 E显微镜提供的应用范围将大大促进 我们对潜在的急性和慢性过程的理解， 功能障碍、疾病和健康下降。此外，它将是VAWNYHS的一个重要工具， 研究人员开发有效的干预措施，以尽量减少严重程度，延迟发病，或逮捕 并最终改善退伍军人群体的生活质量。

Project Abstract The Keyence BZ-X800E All-in-One fluorescent microscope is a unique instrument that provides multi-color fluorescent imaging, 3D image rendering and measurement localization, live cell and time-lapse video generation, automated multi-image collection, and real-time image stitching and image analysis. Thus, the Keyence microscope will be a crucial instrument for VAWNYHS investigators, and the unique features will have immediate benefits for VA-funded projects below. Dr. Troen’s work investigates musculoskeletal aging, frailty, and functional capacity in older veterans. Dr. Troen is conducting both human and animal studies to explore the benefits of high intensity interval training (HIIT) and nutritional supplements on skeletal muscle and bone morphology and function during aging. The Keyence will allow more precise assessments of muscle fibers in a fraction of the time of the current methodology. The Keyence will also be able to generate time-lapse live cell imaging video to observe the formation and activity of bone resorbing osteoclasts, capture the impacts of reagent interventions, and facilitate analysis of bones from experimental animals. Dr. Fliesler’s projects investigate the underlying mechanism of progressive retinal degeneration and visual dysfunction associated with blast overpressure-induced polytrauma, and the impact of novel antioxidants as therapeutic agents to prevent, minimize, or slow the progression of the pathological processes. The Keyence microscope will afford quantitative assessment of histological damage and biomarkers of oxidative stress, cell death, and gliosis. Dr. Sullivan’s study investigates the development of post-transcriptional gene silencing agents such as ribozymes (catalytic RNAs) as candidate therapeutics for orphan retinal degenerations (e.g. retinitis pigmentosa) and common age-related macular degeneration (AMD). The Keyence will provide dramatic new methodologies to observe cellular RNA trafficking and investigate the kinetics and steady-state outcomes of catalytic RNA (ribozyme) therapeutics. Dr. Canty’s study investigates dynamic remodeling during sudden cardiac arrest and cardiac stem cell mediated repair. The Keyence will facilitate characterization of specific hematopoietic subtypes and macrophage polarization and enable characterization of stems in culture to better understand how adult stem cells stimulate endogenous myocyte proliferation. Dr. Feng’s study seeks to understand how mutations of parkin cause the selective degeneration of human nigral dopaminergic neurons and the ensuing Parkinson’s disease. The Keyence will allow the ability to monitor morphological changes and enable imaging of iPSC-derived midbrain DA neurons, to identify impacts of parkin mutations as a model of idiopathic Parkinson’s disease. Dr. Lang’s research seeks to determine the functional role of exosomes in stem cell-mediated cardiac repair. The Keyence microscope will enhance this work by facilitating extremely high resolution immunohistological analysis of murine cardiac tissue following myocardial infarction and evaluating the potential of exosome-based therapy to ameliorate damaged tissues. The range of applications provided by the Keyence BZ-X800E microscope will greatly facilitate our understanding of the underlying acute and chronic processes that result in physiological dysfunction, disease, and health decline. Further, it will be an essential tool for the VAWNYHS researchers to develop effective interventions to minimize the severity, delay the onset, or arrest the progression of frailty and ultimately improve the quality of life in the veteran population.