ShEEP Request for Keyence BZ-X800E All-in-One Fluorescence Microscope

ShEEP 请求购买 Keyence BZ-X800E 一体式荧光显微镜

• 批准号: 9907801
• 负责人: Bruce R. Troen
• 金额: --
• 依托单位: VA WESTERN NEW YORK HEALTHCARE SYSTEM
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-09-01 至 2020-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9907801
• 关键词: Activities of Daily Living; Acute; Age related macular degeneration; Aging; Animals; Antioxidants; Biological Markers; Carbon Dioxide; Cardiac; Catalytic RNA; Cell Death; Cell physiology; Cells; Chronic; Clinic; Collaborations; Collection; Color; Computer software; Controlled Environment; Cultured Cells; Data; Development; Devices; Dimensions; Disease; Equipment; Fluorescence; Functional disorder; Funding; Future; Generations; Gliosis; Health; Heart Arrest; Hematopoietic; Histologic; Human; Idiopathic Parkinson Disease; Image; Image Analysis; Immersion Investigative Technique; Interval training; Intervention; Kinetics; Manufacturer Name; Measurement; Mediating; Methodology; Microscope; Midbrain structure; Modeling; Monitor; Morphology; Mus; Muscle Cells; Muscle Fibers; Musculoskeletal; Mutation; Myocardial Infarction; Oils; Orphan; Osteoclasts; Outcome; Oxidative Stress; Parkinson Disease; Pathologic; Pathologic Processes; Phase; Physiological; Population; Process; Quality of life; RNA; RNA Interference; Reagent; Research; Research Personnel; Resolution; Retinal Degeneration; Retinitis Pigmentosa; Role; Severities; Sheep; Signal Transduction; Skeletal Muscle; Skeletal bone; Slide; Stem cells; Temperature; Therapeutic; Therapeutic Agents; Therapeutic Intervention; Three-Dimensional Image; Three-Dimensional Imaging; Time; Tissues; Translating; Translational Research; Veterans; Visual; Work; adult stem cell; base; bench to bedside; bone; bone resorbing activity; cardiac repair; dietary supplements; dopaminergic neuron; effective intervention; exosome; flasks; fluorescence imaging; fluorescence microscope; fluorophore; frailty; improved; induced pluripotent stem cell; insight; instrument; live cell imaging; macrophage; movie; novel; parkin gene/protein; prevent; real-time images; repaired; stem; therapeutic candidate; tool; trafficking

Project Abstract The Keyence BZ-X800E All-in-One fluorescent microscope is a unique instrument that provides multi-color fluorescent imaging, 3D image rendering and measurement localization, live cell and time-lapse video generation, automated multi-image collection, and real-time image stitching and image analysis. Thus, the Keyence microscope will be a crucial instrument for VAWNYHS investigators, and the unique features will have immediate benefits for VA-funded projects below. Dr. Troen’s work investigates musculoskeletal aging, frailty, and functional capacity in older veterans. Dr. Troen is conducting both human and animal studies to explore the benefits of high intensity interval training (HIIT) and nutritional supplements on skeletal muscle and bone morphology and function during aging. The Keyence will allow more precise assessments of muscle fibers in a fraction of the time of the current methodology. The Keyence will also be able to generate time-lapse live cell imaging video to observe the formation and activity of bone resorbing osteoclasts, capture the impacts of reagent interventions, and facilitate analysis of bones from experimental animals. Dr. Fliesler’s projects investigate the underlying mechanism of progressive retinal degeneration and visual dysfunction associated with blast overpressure-induced polytrauma, and the impact of novel antioxidants as therapeutic agents to prevent, minimize, or slow the progression of the pathological processes. The Keyence microscope will afford quantitative assessment of histological damage and biomarkers of oxidative stress, cell death, and gliosis. Dr. Sullivan’s study investigates the development of post-transcriptional gene silencing agents such as ribozymes (catalytic RNAs) as candidate therapeutics for orphan retinal degenerations (e.g. retinitis pigmentosa) and common age-related macular degeneration (AMD). The Keyence will provide dramatic new methodologies to observe cellular RNA trafficking and investigate the kinetics and steady-state outcomes of catalytic RNA (ribozyme) therapeutics. Dr. Canty’s study investigates dynamic remodeling during sudden cardiac arrest and cardiac stem cell mediated repair. The Keyence will facilitate characterization of specific hematopoietic subtypes and macrophage polarization and enable characterization of stems in culture to better understand how adult stem cells stimulate endogenous myocyte proliferation. Dr. Feng’s study seeks to understand how mutations of parkin cause the selective degeneration of human nigral dopaminergic neurons and the ensuing Parkinson’s disease. The Keyence will allow the ability to monitor morphological changes and enable imaging of iPSC-derived midbrain DA neurons, to identify impacts of parkin mutations as a model of idiopathic Parkinson’s disease. Dr. Lang’s research seeks to determine the functional role of exosomes in stem cell-mediated cardiac repair. The Keyence microscope will enhance this work by facilitating extremely high resolution immunohistological analysis of murine cardiac tissue following myocardial infarction and evaluating the potential of exosome-based therapy to ameliorate damaged tissues. The range of applications provided by the Keyence BZ-X800E microscope will greatly facilitate our understanding of the underlying acute and chronic processes that result in physiological dysfunction, disease, and health decline. Further, it will be an essential tool for the VAWNYHS researchers to develop effective interventions to minimize the severity, delay the onset, or arrest the progression of frailty and ultimately improve the quality of life in the veteran population.

项目摘要 Keyence BZ-X800E 一体式荧光显微镜是一款独特的仪器， 提供多色荧光成像、3D 图像渲染和测量定位、活细胞 和延时视频生成、自动多图像采集以及实时图像拼接和 图像分析。因此，Keyence 显微镜将成为 VAWNYHS 的重要仪器 研究人员认为，这些独特的功能将为以下 VA 资助的项目带来直接好处。 Troen 博士的工作研究了老年人的肌肉骨骼衰老、虚弱和功能能力 退伍军人。 Troen 博士正在进行人体和动物研究，以探索高浓度的好处 强度间歇训练（HIIT）和营养补充剂对骨骼肌和骨骼形态的影响 和衰老过程中的功能。 Keyence 将能够更精确地评​​估肌肉纤维 当前方法的时间的一小部分。 Keyence 还能够生成延时 活细胞成像视频观察骨吸收破骨细胞的形成和活性，捕捉 试剂干预的影响，并促进实验动物骨骼的分析。 Fliesler 博士的项目研究了进行性视网膜变性的潜在机制 和与爆炸超压引起的多发伤相关的视觉功能障碍，以及 新型抗氧化剂作为治疗剂来预防、最小化或减缓疾病的进展 病理过程。 Keyence 显微镜将提供组织学的定量评估 氧化应激、细胞死亡和神经胶质增生的损伤和生物标志物。 沙利文博士的研究调查了转录后基因沉默剂的开发 例如核酶（催化 RNA）作为孤儿视网膜变性（例如视网膜变性）的候选疗法。 视网膜色素变性）和常见的年龄相关性黄斑变性（AMD）。基恩士将 提供引人注目的新方法来观察细胞 RNA 运输并研究动力学 以及催化 RNA（核酶）疗法的稳态结果。 Canty 博士的研究调查了心脏骤停和心脏骤停期间的动态重塑。 干细胞介导的修复。 Keyence 将促进特定造血细胞的表征 亚型和巨噬细胞极化，并能够更好地表征培养物中的茎 了解成体干细胞如何刺激内源性肌细胞增殖。 冯博士的研究旨在了解parkin突变如何导致选择性变性 人类黑质多巴胺能神经元和随后的帕金森病。 Keyence 将允许 能够监测形态变化并实现 iPSC 衍生的中脑 DA 成像 神经元，以确定帕金突变作为特发性帕金森病模型的影响。 Lang 博士的研究旨在确定外泌体在干细胞介导的功能中的功能作用 心脏修复。基恩士显微镜将通过促进极高的 心肌梗死后小鼠心脏组织的分辨率免疫组织学分析 评估基于外泌体的疗法改善受损组织的潜力。 Keyence BZ-X800E 显微镜提供的应用范围将极大地方便 我们对导致生理现象的潜在急性和慢性过程的理解 功能障碍、疾病和健康状况下降。此外，它将成为 VAWNYHS 的重要工具 研究人员制定有效的干预措施，以尽量减少严重程度、推迟发病或阻止病情的发展 衰弱的进展并最终改善退伍军人群体的生活质量。