课题基金 / 基金详情

Dissecting epitranscriptomic signal from complex tissues

Dissecting epitranscriptomic signal from complex tissues
剖析复杂组织的表观转录组信号
批准号:
10184935
负责人:
Hao Wu
金额:
$34.17万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
2021
资助国家:
美国
项目状态:
未结题
起止时间:
2021-09-01 至 2025-07-31

项目摘要

项目成果

Hao Wu的其他基金

相似基金

相关文献

中文摘要
翻译
点击翻译按钮获取中文摘要
英文摘要
Title: Dissecting epitranscriptomic signal from complex tissues PROJECT SUMMARY: “RNA epigenetics” or “epitranscriptomics” has emerged in recent years as an exciting and active research field to study post-transcriptional regulation of gene expressions. The RNA modifications play important roles in gene expression regulation, and are involved in neurodevelopment and a number of neurological diseases. Methylated RNA Immunoprecipitation Sequencing (MeRIP-seq) is a newly developed technology for transcriptome-wise profiling of the RNA epigenetic modifications. MeRIP-seq leads to an expansion of applications in both basic and clinical research, but faces a number of challenges in analysis with its unique data characteristics, including 1) the presence of technical artifacts due to sequence content and sample preparation procedures unique to MeRIP; 2) lack of appropriate methods for identification and comparison of RNA methylated regions; and 3) lack of methods to account for the heterogeneity of tissue samples. In this proposal, we will address these challenges and develop a series of novel statistical methods for MeRIP-seq data preprocessing and analyses. They include a data normalization procedure to remove technical bias, accurate and efficient methods for RNA methylation site detection and comparison, and signal deconvolution methods to draw cell type specific inferences based on data from tissue samples. All methods developed in this project will be implemented and released as free, open source software to benefit the epigenomics research community, including basic scientists working on genetics, epigenetics, gene regulation and cell development, as well as clinicians looking for disease biomarkers.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
Elucidating the functional mechanism of NLRP3 inflammasome activation
  • 批准号:
    10720435
  • 项目类别:
  • 资助金额:
    $70.8万
  • 财政年份:
    2023
  • 负责人:
    Hao Wu
  • 依托单位:
Project #2 Integrated single-nucleus multi-omics (ATAC-seq+RNA-seq or chromatin accessibility + RNA-seq) of human TGs
  • 批准号:
    10806548
  • 项目类别:
  • 资助金额:
    $136.43万
  • 财政年份:
    2023
  • 负责人:
    Hao Wu
  • 依托单位:
Elucidating the structural mechanism of pore formation by the (GSDM) Gasdermin family
  • 批准号:
    10417119
  • 项目类别:
  • 资助金额:
    $51.45万
  • 财政年份:
    2018
  • 负责人:
    Hao Wu
  • 依托单位:
Elucidating the structural mechanism of pore formation by the (GSDM) Gasdermin family
  • 批准号:
    10171760
  • 项目类别:
  • 资助金额:
    $51.45万
  • 财政年份:
    2018
  • 负责人:
    Hao Wu
  • 依托单位:
海外基金