Maintenance of the SPF Breeding Colonies at Yerkes National Primate Research Center

耶基斯国家灵长类研究中心 SPF 繁殖群的维护

• 批准号: 10203159
• 负责人: Joyce Kimberly Cohen
• 金额: $ 79.98万
• 依托单位: EMORY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-30 至 2022-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10203159
• 关键词: 2019-nCoV; AIDS/HIV problem; Acquired Immunodeficiency Syndrome; Alleles; Animal Model; Animals; Biological Assay; Breeding; COVID-19; Code; Development; Diagnostic tests; Disease; Disease Progression; Ensure; Enzyme-Linked Immunosorbent Assay; Fees; Female; Frequencies; Funding; Genes; Genetic; Genetic Screening; Genomic DNA; Genotype; Grant; Growth; HIV; HIV Infections; Health; Immunity; Income; Infection; Leadership; MHC Class I Genes; Macaca mulatta; Maintenance; Methods; Modeling; Molecular; Nose; Pathogen detection; Pathogenicity; Pattern; Primates; Process; Production; Request for Applications; Research; Research Project Grants; Resources; Sampling; Scientist; Serological; Serum; Swab; Techniques; Testing; Text Messaging; United States National Institutes of Health; Vaccines; Variant; Viral; Virus; Virus Diseases; Work; X Chromosome; aged; animal colony; genetic information; genetic pedigree; genome-wide; germ free condition; insight; male; nonhuman primate; preclinical study; prevent; programs; receptor; risk minimization; screening; therapy development; viral detection; working group

The AIDS research portfolio at Yerkes National Primate Research Center (YNPRC) has continued to expand as demonstrated by a growth of approximately $12 million dollars (57% increase) in nonhuman primate (NHP) AIDS research grant funding over the past five years. This growth in grant funding is accompanied by a high demand for Indian-origin specific pathogen free (SPF) rhesus macaques as the animal model for this research. YNPRC has maintained a colony of SPF rhesus macaques (previously with U24 support) in order to provide these animals for HIV/AIDS research. This application requests continued support (now a U42 mechanism) to expand the SPF colony by maximizing production. The application includes the Overview; Husbandry and Management Core; the Viral Testing Core; and the MHC Genetic Typing Core. This grant will support a subset of the overall YNPRC SPF colony that derive program income from animal assignment fees and per diems. YNPRC will provide institutional support to cover remaining expenses for this colony as well as the remainder of the SPF colony. Management and Husbandry: To leverage the resources provided by the U42 to maximize production of Indian-origin rhesus monkeys and facilitate allocation of these animals for NIH-funded HIV/AIDS research. Viral Testing: To provide serological and molecular viral diagnostic testing in support of the SPF colony and to maintain a colony free of diseases that impact AIDS-related research at the Yerkes NPRC. In this supplement we propose to develop and implement molecular and serologic methods for detecting SARS-CoV-2 infection and immunity in the SPF rhesus macaque colony at Yerkes National Primate Research Center. MHC Genetic Typing: To provide parentage testing, comprehensive analysis of MHC class I and class II alleles, and to develop new techniques for detailed genetic characterization to support a genetically healthy breeding colony and provide genetic information on animals essential to the animal assignment process in support of AIDS research. In this supplement we propose to define and determine the frequency of distinct ACE2 coding alleles within the YNPRC rhesus macaque breeding colony. Achieving these aims will ensure the Yerkes NPRC SPF breeding program is healthy, providing the necessary animals to support our HIV/AIDS research program using Indian-origin rhesus monkeys. The sustainability of this critical resource to scientists performing preclinical studies using the rhesus monkey model will have a positive, significant impact on the development of treatments to prevent or cure infection from HIV in people.

耶基斯国家灵长类动物研究中心（YNPRC）的艾滋病研究项目继续扩大 非人灵长类动物（NHP）的增长约为1200万美元（增长57%）， 艾滋病研究基金在过去五年中。这一增长的赠款资金是伴随着高 印度原产的无特定病原体（SPF）恒河猴作为本研究的动物模型的需求。 YNPRC维持了一个SPF恒河猴群（以前有U24支持），以提供 这些动物用于艾滋病研究。此应用程序请求继续支持（现在是U42机制）， 通过最大化产量来扩大SPF菌落。该应用程序包括概述;畜牧业和 管理核心;病毒检测核心;和MHC基因分型核心。这笔赠款将支持一个子集 整个YNPRC SPF群体的收入来自动物分配费和每日津贴。 YNPRC将提供机构支持，以支付该殖民地的剩余费用以及剩余费用 SPF的殖民地。 管理和饲养：利用U42提供的资源， 印度恒河猴，并促进分配这些动物用于NIH资助的HIV/AIDS研究。 病毒检测：提供血清学和分子病毒诊断检测，以支持SPF菌落， 在耶基斯NPRC保持一个没有影响艾滋病相关研究的疾病的殖民地。在这 我们建议开发和实施分子和血清学方法， Yerkes国家灵长类动物园SPF猕猴群SARS-CoV-2感染与免疫 研究中心 MHC基因分型：提供亲子鉴定，综合分析MHC I类和II类 等位基因，并开发新的技术，详细的遗传特征，以支持遗传健康 繁殖群体，并提供对动物分配过程至关重要的动物遗传信息， 支持艾滋病研究。在本附录中，我们建议定义和确定 在YNPRC恒河猴繁殖群体内的不同ACE 2编码等位基因。 实现这些目标将确保耶基斯NPRC SPF育种计划是健康的，提供必要的 动物来支持我们的艾滋病毒/艾滋病研究计划，使用印度恒河猴。的可持续性 对于使用恒河猴模型进行临床前研究的科学家来说， 对预防或治愈人类艾滋病毒感染的治疗方法的发展产生了积极、重大的影响。