Husbandry and Management Core-Maintenance of the SPF Breeding Colonies at Yerkes National Primate Research Center

项目摘要/摘要 畜牧业与管理核心 耶克斯国家灵长类动物研究中心(YNPRC)在繁殖方面有着悠久而成功的历史 印度原产恒河猴，用于艾滋病毒/艾滋病研究。这种成功在一定程度上可以归功于持续不断的 U24/U42支持这个殖民地的管理和照料。自2002年以来，这种支持让恒河猴 Yerkes NPRC野战站的猕猴繁殖群体建立群体谱系，过渡到完全 SPF状态，建立MHC分析，并验证快速内部B型疱疹病毒(猕猴疱疹病毒 1)筛查方案。在过去的4年里，由国立卫生研究院资助的与艾滋病毒/艾滋病有关的个别项目的数量 对SPF恒河猴的需求相对稳定，我们现在看到冠状病毒感染后的增加 在动物的要求中。因此，目前U42畜牧管理核心的主要目标 应用是利用U42提供的资源来最大限度地生产SPF印度恒河猴 并为NIH资助的艾滋病毒/艾滋病研究分配这些动物提供便利。作为目标1的一部分， 畜牧和管理核心将继续保持SPF状态和遗传健康以及 群体的身体和社会健康，以培育出具有所需MHC基因类型的高质量动物 用于艾滋病毒/艾滋病研究。作为目标2最大化动物生产潜力的一部分，畜牧业和 管理核心将修订提高未来年轻雄性育种者社会能力的战略 为了形成多个雄性育种者群体，提高雄性引种的成功率，并实施 社会网络分析方法，以评估我们大型复合饲养群体的稳定性，例如 母系碎片化，以改善社会稳定，避免社会不稳定带来的不利影响 在制作中。最后，作为目标3采取综合方法管理群体数据集的一部分， 畜牧业和管理核心将从存储社会化和社会行为数据集过渡到 在划分的电子表格中，而不是将它们存储在可通过Web访问的殖民地信息学资源中 并实施项目管理仪表板，以便于处理和汇总以下方面的数据 动物的可获得性、请求和任务，以促进跟踪和报告。这些目标的成功 将确保YNPRC的SPF菌落继续增长，并提供可靠的健康和 基因特征良好的恒河猴用于艾滋病毒/艾滋病研究。

Project Summary/Abstract Husbandry and Management Core The Yerkes National Primate Research Center (YNPRC) has a long and successful history of breeding rhesus macaques of Indian origin for HIV/AIDS research. This success can be attributed, in part, to continuous U24/U42 support for the management and care of this colony. Since 2002, this support has allowed the rhesus macaque breeding colony at the Yerkes NPRC Field Station to establish a colony pedigree, to transition to full SPF status, to institute MHC profiling, and to validate a rapid in-house Herpes B virus (Macacine herpesvirus 1) screening protocol. Over the past 4 years, the number of individual NIH-funded HIV/AIDS-related projects requiring SPF rhesus monkeys has remained relatively steady, and we are now seeing a post-COVID increase in animal requests. Thus, the main objective of the Husbandry and Management Core for the current U42 application is to leverage the resources provided by the U42 to maximize production of SPF Indian rhesus monkeys and facilitate allocation of these animals for NIH-funded HIV/AIDS research. As part of Aim 1, the Husbandry and Management Core will continue to maintain the SPF status and genetic health as well as the physical and social health of the Colony in order to produce high quality animals with desired MHC genotypes for HIV/AIDS research. As part of Aim 2 to maximize animal production potential, the Husbandry and Management Core will revise the strategies for improving the social competence of young future male breeders and for forming multi-male cohorts of breeders to improve the success of male introductions, and implement a social network analytical approach to assess stability in our large, compound-housed breeding groups, such as fragmentation in matrilines, to improve social stability and avoid the detrimental effect that social instability has on production. Finally, as part of Aim 3 to take an integrated approach to managing colony data sets, the Husbandry and Management Core will transition away from storing socialization and social behavioral datasets in compartmentalized spreadsheets to instead store them in the web-accessible Colony Informatics resource and implement a project management dashboard to facilitate manipulation and summary of data regarding animal availability, requests, and assignments to facilitate tracking and reporting. The success of these aims will ensure that the SPF colony at YNPRC will continue to grow and provide a reliable supply of healthy and genetically well-characterized rhesus macaques for HIV/AIDS research.