Maintenance of the SPF Breeding Colonies at Yerkes National Primate Research Center

摘要 耶克斯国家灵长类动物研究中心(YNPRC)的艾滋病研究组合继续扩大 非人灵长类的峰值增长约为650万美元(增长30%)就证明了这一点 (NHP)过去八年的艾滋病研究拨款。赠款资金的增长伴随着 非常需要印度血统的无特定病原体(SPF)猕猴作为这方面的动物模型 研究。YNPRC继续维持一群SPF恒河猴，为这些动物提供 艾滋病毒/艾滋病研究。此应用程序要求继续提供支持，以通过最大化 制作。应用包括概述；畜牧管理核心；病毒检测核心； 和MHC基因分型核心(群体遗传学)。这笔赠款将支持整个YNPRC项目的一部分 SPF殖民地，并将从动物分配费和按日收费中获得计划收入。YNPRC将提供 机构支持，以支付该殖民地以及SPF殖民地其余部分的剩余费用。 管理和畜牧业：利用U42提供的资源，最大限度地提高 印度原产恒河猴，并为NIH资助的艾滋病毒/艾滋病研究分配这些动物提供便利 病毒检测：提供血清学和分子病毒诊断检测，以支持SPF菌落，并 在耶尔克斯NPRC保持一个没有影响艾滋病相关研究的疾病的群体 群体遗传学：提供亲子鉴定，MHC I类和II类等位基因的综合分析， 并开发详细的基因特征的新技术，以支持基因健康的育种 并提供对动物分配过程至关重要的动物遗传信息，以支持 艾滋病研究。 实现这些目标将确保Yerkes NPRC SPF育种计划是可持续的，提供 使用印度血统的恒河猴支持艾滋病毒/艾滋病研究计划所需的动物。这个 使用恒河猴模型进行临床前研究的科学家可获得这一关键资源 将对预防或治愈艾滋病毒感染的治疗方法的发展产生积极而重大的影响 在人们身上。

Abstract The AIDS research portfolio at Yerkes National Primate Research Center (YNPRC) has continued to expand as demonstrated by a peak growth of approximately $6.5 million dollars (30% increase) in nonhuman primate (NHP) AIDS research grant funding over the past eight years. This growth in grant funding is accompanied by a high demand for Indian-origin specific pathogen free (SPF) rhesus macaques as the animal model for this research. YNPRC has continued to maintain a colony of SPF rhesus macaques to provide these animals for HIV/AIDS research. This application requests continued support to expand the SPF colony by maximizing production. The application includes the Overview; Husbandry and Management Core; the Viral Testing Core; and the MHC Genetic Typing Core (Colony Genetics). This grant will support a subset of the overall YNPRC SPF colony and will derive program income from animal assignment fees and per diems. YNPRC will provide institutional support to cover remaining expenses for this colony as well as the remainder of the SPF colony. Management and Husbandry: To leverage the resources provided by the U42 to maximize production of Indian-origin rhesus monkeys and facilitate allocation of these animals for NIH-funded HIV/AIDS research Viral Testing: To provide serological and molecular viral diagnostic testing in support of the SPF colony and to maintain a colony free of diseases that impact AIDS-related research at the Yerkes NPRC Colony Genetics: To provide parentage testing, comprehensive analysis of MHC class I and class II alleles, and to develop new techniques for detailed genetic characterization to support a genetically healthy breeding colony and provide genetic information on animals essential to the animal assignment process in support of AIDS research. Achieving these aims will ensure the Yerkes NPRC SPF breeding program is sustainable, providing the necessary animals to support the HIV/AIDS research program using Indian-origin rhesus monkeys. The availability of this critical resource to scientists performing preclinical studies using the rhesus monkey model will have a positive, significant impact on the development of treatments to prevent or cure infection from HIV in people.