SPF Breeding Colonies at the Yerkes NPRC

Yerkes NPRC 的 SPF 育种群

• 批准号: 9513118
• 负责人: Joyce Kimberly Cohen
• 金额: $ 90.33万
• 依托单位: EMORY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-30 至 2018-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9513118
• 关键词: AIDS prevention; AIDS/HIV problem; Acquired Immunodeficiency Syndrome; Age; Animals; Breeding; Data; Development; Ensure; Equilibrium; Funding; Generations; Genetic; Genetic Enhancement; Genetic Variation; Genotype; Growth; HIV Infections; Health; Human; Human Resources; Income; Institution; Laboratories; Macaca; Macaca mulatta; Major Histocompatibility Complex; Methodology; Phase; Population; Practice Management; Pre-Clinical Model; Primates; Production; Research; Research Personnel; Resources; SIV; Sales; Site; Support Groups; Testing; Tissues; Type D Retrovirus; United States National Institutes of Health; Universities; Virus; Virus Diseases; age stratification; aged; base; cost; cost effective; experimental study; genetic information; genetic pedigree; germ free condition; improved; juvenile animal; member; nonhuman primate; population stratification; prevent; programs; public health relevance; response; screening; seroconversion; social; virology

DESCRIPTION (provided by applicant): The development of effective strategies to prevent and treat HIV infection is of paramount importance to improve global human health. Simian immunodeficiency virus (SIV) infection of rhesus macaques (Macaca mulatta) is the most effective pre-clinical model of HIV infection. Members of the Macaca genus, however, harbor several viruses that could confound experimental results from studies relating to the AIDS prevention or cure. It is therefore vital to ensure a constant supply of specific pathogen free (SPF) rhesus macaques for experimental studies in AIDS research. Since 2002, the SPF Rhesus macaque breeding groups at Yerkes National Primate Research Center (YNPRC) have, in part, been supported by U24 funding. By the end of 2013, all breeding groups will be free of SIV, Simian T-Lymphotropic Virus (STLV), Simian Type D Retroviruses (SRV), and Herpes Simian B Virus (Herpes-B). The central aim of this final phase of U24 funding is to achieve financial sustainability for those U24-supported groups and to expand multi-generational, genetically characterized breeding groups to provide appropriately aged experimental animals for NIH funded AIDS research. To achieve this aim, proven management practices will be employed that allow optimum production of animals in socially stable breeding groups to provide animals to AIDS-related studies. A second aim of this project is to expand methodology for SRV and Herpes testing in the Yerkes Virology Core. On-site SRV and Herpes B testing will save costs and allow a more rapid colony management response should the virus status of any SPF animal be questionable. A third aim of this renewal is to transition to a more cost-effective, therefore financially sustainable, SNP-based and direct sequencing approach for macaque genotyping, providing AIDS investigators with pedigreed animals with confirmed sets of major histocompatibility complex (MHC). It is expected that the YNPRC SPF rhesus macaque breeding colony will be financially sustainable by the end of the next U24 funding period and will be recognized as a national resource of genetically well-characterized rhesus macaques available for HIV/AIDS research.

描述（由申请人提供）：制定预防和治疗艾滋病毒感染的有效策略对于改善全球人类健康至关重要。鼻猕猴（Macaca Mulatta）的猿猴免疫缺陷病毒（SIV）感染是HIV感染的最有效的临床前模型。然而，Macaca属的成员藏有几种病毒，这些病毒可能会使与预防或治愈有关的研究混淆实验结果。因此，至关重要的是要确保持续供应特定的无病原体（SPF）猕猴，以进行艾滋病研究的实验研究。自2002年以来，Yerkes国家灵长类动物研究中心（YNPRC）的SPF恒河猕猴育种团体部分得到了U24资金的支持。到2013年底，所有育种组都将脱离SIV，Simian T淋巴细胞病毒（STLV），D型D型逆转录病毒（SRV）和疱疹Simian B病毒（Herpes-b）。 U24资金的最后阶段的核心目的是为那些由U24支持的群体实现财务可持续性，并扩大多代，遗传特征的育种群体，以为NIH资助的艾滋病研究提供适当老化的实验动物。为了实现这一目标，将采用经过验证的管理实践，以使社会稳定育种群体中的动物最佳生产为与艾滋病相关的研究提供动物。该项目的第二个目的是扩展Yerkes病毒核心中SRV和疱疹测试的方法。现场SRV和疱疹B测试将节省成本，并在任何SPF动物的病毒状况值得怀疑的情况下允许更快的殖民地管理反应。这种更新的第三个目的是向更具成本效益的，基于SNP的经济可持续性，基于SNP的直接测序方法过渡，以提供艾滋病研究人员为研究人员提供了一系列确认的主要组织相容性复合物（MHC）。预计YNPRC SPF SPF猕猴育种殖民地将在下一个U24融资期结束前在财务上可持续发展，并将被视为可用于遗传性良好的恒河猴的国家资源，可用于HIV/AIDS研究。