SPF Breeding Colonies at the Yerkes NPRC

描述(由申请者提供)：制定有效的战略来预防和治疗艾滋病毒感染对改善全球人类健康至关重要。猴免疫缺陷病毒(SIV)感染恒河猴(Macaca Mulatta)是临床前最有效的HIV感染模型。然而，猕猴属的成员携带着几种病毒，这些病毒可能会混淆与艾滋病预防或治疗相关研究的实验结果。因此，在艾滋病研究的实验研究中，确保恒定供应无特定病原体(SPF)的猕猴是至关重要的。自2002年以来，耶克斯国家灵长类研究中心(YNPRC)的SPF猕猴繁育小组部分得到了U24基金的支持。到2013年底，所有养殖群将不再有SIV、猿猴T淋巴病毒(STLV)、猿猴D型逆转录病毒(SRV)和猿猴B型疱疹病毒(疱疹-B)。U24资助的这一最后阶段的中心目标是为那些由U24资助的群体实现财务可持续性，并扩大多代、具有基因特征的繁殖群体，为NIH资助的艾滋病研究提供适当年龄的实验动物。为了实现这一目标，将采用行之有效的管理做法，允许在社会稳定的繁育群体中最佳地生产动物，以便为与艾滋病有关的研究提供动物。该项目的第二个目标是在耶克斯病毒学核心扩展SRV和疱疹病毒检测的方法学。现场SRV和B型疱疹病毒检测将节省成本，并在任何SPF动物的病毒状态有问题时，允许更快的菌落管理反应。这一更新的第三个目标是过渡到更具成本效益的、因此在经济上可持续的、基于SNP的直接测序的猕猴基因分型方法，为艾滋病研究人员提供纯种动物确认的主要组织相容性复合体(MHC)。预计YNPRC SPF猕猴繁殖群体将在下一个U24资助期结束时在财务上可持续，并将被公认为可用于艾滋病毒/艾滋病研究的具有良好遗传特征的全国恒河猴资源。

DESCRIPTION (provided by applicant): The development of effective strategies to prevent and treat HIV infection is of paramount importance to improve global human health. Simian immunodeficiency virus (SIV) infection of rhesus macaques (Macaca mulatta) is the most effective pre-clinical model of HIV infection. Members of the Macaca genus, however, harbor several viruses that could confound experimental results from studies relating to the AIDS prevention or cure. It is therefore vital to ensure a constant supply of specific pathogen free (SPF) rhesus macaques for experimental studies in AIDS research. Since 2002, the SPF Rhesus macaque breeding groups at Yerkes National Primate Research Center (YNPRC) have, in part, been supported by U24 funding. By the end of 2013, all breeding groups will be free of SIV, Simian T-Lymphotropic Virus (STLV), Simian Type D Retroviruses (SRV), and Herpes Simian B Virus (Herpes-B). The central aim of this final phase of U24 funding is to achieve financial sustainability for those U24-supported groups and to expand multi-generational, genetically characterized breeding groups to provide appropriately aged experimental animals for NIH funded AIDS research. To achieve this aim, proven management practices will be employed that allow optimum production of animals in socially stable breeding groups to provide animals to AIDS-related studies. A second aim of this project is to expand methodology for SRV and Herpes testing in the Yerkes Virology Core. On-site SRV and Herpes B testing will save costs and allow a more rapid colony management response should the virus status of any SPF animal be questionable. A third aim of this renewal is to transition to a more cost-effective, therefore financially sustainable, SNP-based and direct sequencing approach for macaque genotyping, providing AIDS investigators with pedigreed animals with confirmed sets of major histocompatibility complex (MHC). It is expected that the YNPRC SPF rhesus macaque breeding colony will be financially sustainable by the end of the next U24 funding period and will be recognized as a national resource of genetically well-characterized rhesus macaques available for HIV/AIDS research.