SPF Breeding Colonies at the Yerkes NPRC

描述（由申请人提供）：制定预防和治疗HIV感染的有效策略对改善全球人类健康至关重要。猴免疫缺陷病毒（SIV）感染恒河猴（Macacamulatta）是最有效的HIV感染的临床前模型。然而，猕猴属的成员携带着几种病毒，这些病毒可能会混淆与艾滋病预防或治疗相关的研究的实验结果。因此，确保无特定病原体（SPF）恒河猴的持续供应对于艾滋病研究的实验研究至关重要。自2002年以来，耶基斯国家灵长类动物研究中心（YNPRC）的SPF恒河猴繁殖组部分得到了U24资金的支持。到2013年底，所有繁殖组将没有SIV、猿猴嗜T细胞病毒（STLV）、猿猴D型逆转录病毒（SRV）和猿猴B疱疹病毒（疱疹-B）。U24资助的最后阶段的中心目标是为U24支持的团体实现财务可持续性，并扩大多代遗传特征的育种群体，为NIH资助的艾滋病研究提供适当年龄的实验动物。为了实现这一目标，将采用经过验证的管理做法，在社会稳定的繁殖群体中实现动物的最佳生产，为艾滋病相关研究提供动物。该项目的第二个目的是扩展耶基斯病毒学核心中的SRV和疱疹检测方法。现场SRV和B疱疹检测将节省成本，并在任何SPF动物的病毒状态存在疑问时允许更快速的菌落管理响应。此次更新的第三个目的是过渡到一个更具成本效益的，因此在财政上可持续的，基于SNP和直接测序的猕猴基因分型方法，为艾滋病研究人员提供具有确认的主要组织相容性复合体（MHC）的纯种动物。预计到下一个U24资助期结束时，YNPRC SPF恒河猴繁殖群将在财政上可持续，并将被公认为可用于艾滋病毒/艾滋病研究的遗传特征良好的恒河猴国家资源。

DESCRIPTION (provided by applicant): The development of effective strategies to prevent and treat HIV infection is of paramount importance to improve global human health. Simian immunodeficiency virus (SIV) infection of rhesus macaques (Macaca mulatta) is the most effective pre-clinical model of HIV infection. Members of the Macaca genus, however, harbor several viruses that could confound experimental results from studies relating to the AIDS prevention or cure. It is therefore vital to ensure a constant supply of specific pathogen free (SPF) rhesus macaques for experimental studies in AIDS research. Since 2002, the SPF Rhesus macaque breeding groups at Yerkes National Primate Research Center (YNPRC) have, in part, been supported by U24 funding. By the end of 2013, all breeding groups will be free of SIV, Simian T-Lymphotropic Virus (STLV), Simian Type D Retroviruses (SRV), and Herpes Simian B Virus (Herpes-B). The central aim of this final phase of U24 funding is to achieve financial sustainability for those U24-supported groups and to expand multi-generational, genetically characterized breeding groups to provide appropriately aged experimental animals for NIH funded AIDS research. To achieve this aim, proven management practices will be employed that allow optimum production of animals in socially stable breeding groups to provide animals to AIDS-related studies. A second aim of this project is to expand methodology for SRV and Herpes testing in the Yerkes Virology Core. On-site SRV and Herpes B testing will save costs and allow a more rapid colony management response should the virus status of any SPF animal be questionable. A third aim of this renewal is to transition to a more cost-effective, therefore financially sustainable, SNP-based and direct sequencing approach for macaque genotyping, providing AIDS investigators with pedigreed animals with confirmed sets of major histocompatibility complex (MHC). It is expected that the YNPRC SPF rhesus macaque breeding colony will be financially sustainable by the end of the next U24 funding period and will be recognized as a national resource of genetically well-characterized rhesus macaques available for HIV/AIDS research.