Maintenance of the SPF Breeding Colonies at Yerkes National Primate Research Center

项目总结/摘要 耶基斯国家灵长类动物研究中心（YNPRC）的艾滋病研究项目继续扩大 非人灵长类动物（NHP）的增长约为1200万美元（增长57%）， 艾滋病研究基金在过去五年中。这一增长的赠款资金是伴随着高 印度原产的无特定病原体（SPF）恒河猴作为本研究的动物模型的需求。 YNPRC维持了一个SPF恒河猴群（以前有U24支持），以提供 这些动物用于艾滋病研究。此应用程序请求继续支持（现在是U42机制）， 通过最大化产量来扩大SPF菌落。该应用程序包括概述;畜牧业和 管理核心;病毒检测核心;和MHC基因分型核心。这笔赠款将支持一个子集 整个YNPRC SPF群体的动物分配费和每日津贴将获得项目收入。 YNPRC将提供机构支持，以支付该殖民地的剩余费用以及剩余费用 SPF的殖民地。 管理和饲养：利用U42提供的资源， 印度恒河猴，并促进分配这些动物用于NIH资助的HIV/AIDS研究。 病毒检测：提供血清学和分子病毒诊断检测，以支持SPF菌落， 在耶基斯NPRC保持一个没有影响艾滋病相关研究的疾病的殖民地。 MHC基因分型：提供亲子鉴定，综合分析MHC I类和II类 等位基因，并开发新的技术，详细的遗传特征，以支持遗传健康 繁殖群体，并提供对动物分配过程至关重要的动物遗传信息， 支持艾滋病研究。 实现这些目标将确保耶基斯NPRC SPF育种计划是可持续的， 我们需要必要的动物来支持我们的艾滋病毒/艾滋病研究计划，使用印度恒河猴。的 使用恒河猴模型进行临床前研究的科学家可获得这一关键资源 将对预防或治愈艾滋病毒感染的治疗方法的发展产生积极的重大影响 在人身上。

Project Summary/Abstract The AIDS research portfolio at Yerkes National Primate Research Center (YNPRC) has continued to expand as demonstrated by a growth of approximately $12 million dollars (57% increase) in nonhuman primate (NHP) AIDS research grant funding over the past five years. This growth in grant funding is accompanied by a high demand for Indian-origin specific pathogen free (SPF) rhesus macaques as the animal model for this research. YNPRC has maintained a colony of SPF rhesus macaques (previously with U24 support) in order to provide these animals for HIV/AIDS research. This application requests continued support (now a U42 mechanism) to expand the SPF colony by maximizing production. The application includes the Overview; Husbandry and Management Core; the Viral Testing Core; and the MHC Genetic Typing Core. This grant will support a subset of the overall YNPRC SPF colony and will derive program income from animal assignment fees and per diems. YNPRC will provide institutional support to cover remaining expenses for this colony as well as the remainder of the SPF colony. Management and Husbandry: To leverage the resources provided by the U42 to maximize production of Indian-origin rhesus monkeys and facilitate allocation of these animals for NIH-funded HIV/AIDS research. Viral Testing: To provide serological and molecular viral diagnostic testing in support of the SPF colony and to maintain a colony free of diseases that impact AIDS-related research at the Yerkes NPRC. MHC Genetic Typing: To provide parentage testing, comprehensive analysis of MHC class I and class II alleles, and to develop new techniques for detailed genetic characterization to support a genetically healthy breeding colony and provide genetic information on animals essential to the animal assignment process in support of AIDS research. Achieving these aims will ensure the Yerkes NPRC SPF breeding program is sustainable, providing the necessary animals to support our HIV/AIDS research program using Indian-origin rhesus monkeys. The availability of this critical resource to scientists performing preclinical studies using the rhesus monkey model will have a positive, significant impact on the development of treatments to prevent or cure infection from HIV in people.