Neuroimaging Core

抽象核心B 成像核心B(导演保罗·汤普森)为项目1-4提供脑图像分析，这些项目分析 对空气污染的神经退行性反应。次核心B1(M.Braskie)支持关于人类的项目1和2 脑部磁共振图像。亚核B2(B.Zlokovic)支持关于小鼠大脑的项目3和4。 亚核B1人体神经成像 目标1：在ADNI、Whims和Vetsa队列中执行标准化的结构图像分析，以评估 选定的阿尔茨海默病(AD)相关皮质下结构的体素皮质厚度、体积 (海马体和杏仁核)，以及使用Freesurfer软件的区域白质。我们还将评估白色 物质超强度体积。在ADNI和Vetsa中，我们将分析扩散张量成像扫描。 目标2：使用机器学习来生成结构磁共振皮质信号和相关的预测风险 与临床前AD、轻度认知障碍和AD风险相关的分数。 目标3：支持项目1和2在其拟议的调解分析中使用两种有针对性的方法 方程模型(SEM)和高维分析的不可知论方法。 次核心B2，小鼠脑成像。 目的1.在体小鼠脑成像检测脑血流量、血脑屏障(BBB) 渗透性、血管造影术和核磁共振血管造影术。 目的2.用免疫荧光共聚焦显微镜对神经血管单位&血脑屏障进行尸检研究。 目的3.超高场磁共振通过弥散系数图和声道成像图检测小鼠连接性的变化 动态增强(DCE)-MRI测定血脑屏障通透性。 目的4分析项目3和4中的鼠标数据。

Abstract Core B The Imaging Core B (Paul Thompson, Director) provides brain image analysis for Projects 1-4, which analyze neurodegenerative responses to air pollution. Sub-core B1 (M. Braskie) supports Projects 1 and 2 on human brain MR images. Sub-core B2 (B. Zlokovic) supports Projects 3 and 4 on mouse brain. Sub-core B1 Human Neuroimaging Aim 1: Perform standardized structural image analyses in the ADNI, WHIMS and VETSA cohorts to assess voxelwise cortical thickness, volumes of selected Alzheimer's disease (AD)-relevant subcortical structures (hippocampus and amygdala), and regional white matter using FreeSurfer software. We will also assess white matter hyperintensity volumes. In ADNI and VETSA we will analyze diffusion tensor imaging scans. Aim 2: Use machine learning to generate structural MRI cortical signatures and associated predicted risk scores related to risk for preclinical AD, mild cognitive impairment, and AD. Aim 3: Support Projects 1 & 2 in their proposed mediation analyses using both targeted approach in structural equation models (SEM) and agnostic approach with high-dimensional analyses. Sub-Core B2, Mouse brain imaging. Aim 1. Perform in vivo mouse brain imaging to assess cerebral blood flow, blood brain barrier (BBB) permeability, vascular angiography, and MRI tractography. Aim 2. Postmortem studies of the neurovascular unit & BBB by confocal microscopy for immunofluorescence. Aim 3. Ultra-highfield MRI to detect mouse connectivity changes through diffusivity and tractography maps & quantification of BBB permeability by dynamic contrast enhanced (DCE)-MRI. Aim 4 Analyze mouse data from Projects 3 and 4.