Core-005

核心005

• 批准号: 10303167
• 负责人: CARYN LERMAN
• 金额: $ 63.26万
• 依托单位: UNIVERSITY OF SOUTHERN CALIFORNIA
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-12-01 至 2021-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10303167
• 关键词: Advanced Malignant Neoplasm; Cancer Center; Cancer Center Support Grant; Cells; Center Core Grants; Collaborations; Computer Assisted; Consultations; Continuing Education; Cryoultramicrotomy; Education; Equipment; Fluorescence; Fluorescence Microscopy; Funding; Goals; Image; Imaging Device; Imaging technology; International; Knowledge; Lasers; Leadership; Learning; Light; Methodology; Microscopy; Microtomy; Mission; Phase; Protocols documentation; Research; Research Personnel; Resolution; Resources; Scanning; Scanning Electron Microscopy; Services; Supervision; Techniques; Technology; Tissue imaging; Training Programs; Training and Education; Transmission Electron Microscopy; University of Southern California Norris Cancer Center; anticancer research; cellular imaging; confocal imaging; digital; experience; falls; fluorescence imaging; fluorescence microscope; imager; imaging capabilities; innovation; instrumentation; laser capture microdissection; light microscopy; member; multiphoton microscopy; new technology; novel; programs; recruit; single molecule; two-photon

The Cell and Tissue Imaging Core provides advanced cell and tissue imaging technology, services, and scientific consultation. The Core facilitates scientific collaboration and advances the ability of USC Norris members to conduct innovative cancer research. Since its founding 15 years ago, the Cell and Tissue Imaging Core has been continuously approved and funded by the NCI CCSG. The facility has been ably led by the founding Director, Dr. David Hinton, and supervised by Technical Director, Ernesto Barron, both of whom have over 30 years of experience in the field of microscopy. In 2014, Dr. Scott Fraser, an internationally recognized innovator in imaging technology, was recruited to USC from Caltech. With the goal of providing national leadership in imaging technology through the facility, Dr. Fraser joined Dr. Hinton as Co-Director effective January 2015. The Cell and Tissue Imaging Core makes it possible for Cancer Center members to easily access a wide range of sophisticated imaging equipment and services, including laser scanning confocal/multiphoton microscopy, live cell spinning disk confocal imaging, transmission electron microscopy (TEM), scanning electron microscopy (SEM), digital light and fluorescence microscopy, fluorescence and bright field laser capture microdissection, thin sectioning, cryo-sectioning and embedding techniques, and computer aided graphics. In its effort to provide state-of-the-art technology, the Core is adding new instrumentation: a STORM super-resolution single-molecule imaging fluorescence microscope, a Two-Photon SPIM light sheet imager, a Flip Trap imaging scope, and a novel Hyperspectral Phasor Imaging (HPI) instrument. As a part of its mission to help members learn how Core resources can advance their research, the Core has an active education and training program through which it advises and instructs USC Norris investigators in novel methodologies and protocols to enhance their cell and tissue imaging capability. In FY 2013-2014, the Core was used by 36 Cancer Center members from six USC Norris Research Programs, which reflects both the quality of the service and the value of continued education and training efforts. In the fall of 2015, the Core will relocate to renovated space in USC Norris Topping Tower. Closer proximity to the majority of users and increased overall visibility should result in increased usage in the next project period.

细胞和组织成像核心提供先进的细胞和组织成像技术、服务和 科学咨询。核心促进科学合作并提高南加州大学诺里斯分校的能力 成员进行创新的癌症研究。自 15 年前成立以来，细胞和组织成像 Core 不断得到 NCI CCSG 的批准和资助。该设施得到了 创始人为 David Hinton 博士，技术总监为 Ernesto Barron，两人均拥有 在显微镜领域拥有超过 30 年的经验。 2014 年，国际公认的斯科特·弗雷泽 (Scott Fraser) 博士 成像技术的创新者，从加州理工学院招聘到南加州大学。以向全国提供 通过该设施在成像技术方面处于领先地位，弗雷泽博士与辛顿博士一起担任联合主任，有效 2015 年 1 月。 细胞和组织成像核心使癌症中心成员能够轻松访问广泛的 一系列先进的成像设备和服务，包括激光扫描共焦/多光子 显微镜、活细胞转盘共聚焦成像、透射电子显微镜 (TEM)、扫描 电子显微镜 (SEM)、数字光学和荧光显微镜、荧光和明场激光 捕获显微切割、薄切片、冷冻切片和包埋技术以及计算机辅助 图形。为了提供最先进的技术，Core 正在添加新的仪器：STORM 超分辨率单分子成像荧光显微镜、双光子 SPIM 光片成像仪、 Flip Trap 成像镜和新型高光谱相量成像 (HPI) 仪器。作为其使命的一部分 为了帮助成员了解核心资源如何推进他们的研究，核心有积极的教育和 培训计划，通过该计划向南加州大学诺里斯分校的研究人员提供新方法和指导方面的建议和指导 增强细胞和组织成像能力的协议。 2013-2014 财年，来自 6 个南加州大学诺里斯研究项目的 36 名癌症中心成员使用了 Core 这既反映了服务质量，也反映了继续教育和培训工作的价值。在 2015 年秋季，核心区将搬迁至 USC Norris Topping Tower 翻新后的空间。更接近 大多数用户和整体可见性的提高应该会导致下一个项目期间的使用量增加。