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Cell & Molecular Imaging Core

细胞

基本信息

批准号：
10005397
负责人：
John J Lemasters
金额：
$ 15.32万
依托单位：
MEDICAL UNIVERSITY OF SOUTH CAROLINA
依托单位国家：
美国
项目类别：
财政年份：
2011
资助国家：
美国
起止时间：
2011-09-01 至 2022-01-15
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10005397
关键词：
3-Dimensional 4D Imaging Animals Apoptosis Applications Grants Area Biological Assay Brightfield Microscopy Cancer Biology Cancer Center Cell Death Cell Membrane Permeability Cell Respiration Cell Survival Cells Cellular biology Centers of Research Excellence Charge Computer Workstations Computer software Confocal Microscopy Consultations Cultured Cells Data Analyses Doctor of Philosophy Educational workshop Equilibrium Equipment Fluorescence Fluorescence Resonance Energy Transfer Funding Gases Gene Expression Generations Grant Green Fluorescent Proteins Hour Image Image Analysis Imaging technology Immersion Immunohistochemistry Incubators Individual Injury Ions Journals Label Lasers Leukocytes Ligation Measurement Mentors Methodology Microcirculation Microscope Microscopy Mitochondria Modernization Monitor National Cancer Institute Natural regeneration Necrosis Nitrogen Optics Oral Organism Oxidants Oxidation-Reduction Oxidative Stress Oxygen Permeability Phase Physics Preparation Proteins Publications Reader Reporting Research Personnel Resolution Resources Sampling Scanning Services Signal Transduction Silicone Oils South Carolina Specimen Stress System Techniques Temperature Tissue Sample Tissue Stains Tissues Training United States National Institutes of Health Water base cellular imaging charge coupled device camera confocal imaging digital drug discovery electrical potential equipment acquisition experience experimental study fluorescence imaging fluorescence microscope fluorophore imaging capabilities imaging facilities imaging software instrumentation intravital imaging intravital microscopy live cell imaging meetings molecular imaging movie multiphoton imaging multiphoton microscopy novel therapeutics photomultiplier posters protein distribution pyridine nucleotide quasar success tissue culture

项目摘要

Cell & Molecular Imaging Core D —John Lemasters, MD, PhD, Core Leader Abstract Cell & Molecular Imaging Core D provides major support in confocal, multiphoton and brightfield microscopy to COBRE investigators. The Imaging Core houses the following major microscope systems: 1) a Zeiss LSM 880 NLO confocal/multiphoton microscope equipped with a Coherent Chameleon multiphoton laser with Quasar photomultiplier array for spectral analysis; 2) an Olympus FV1200 multiphoton microscope with Spectra- Physics MaiTai multiphoton laser and silicone oil optics for intravital imaging, 3) an Olympus FV10i LIV live cell confocal microscope with water immersion optics, 4) a Zeiss LSM 510 META laser scanning confocal microscope; and 5) a BD CARV II disk-scanning confocal microscope for video-rate imaging as well as conventional widefield microscopy. All microscopes are equipped with environmental chambers for temperature and gas phase control to allow high resolution non-destructive 3-dimensional imaging of living cells, tissues and organisms. Major applications of this instrumentation include 1) live cell imaging of parameter-sensitive fluorophores to monitor ions, electrical potentials, oxygen and nitrogen radical generation, pyridine nucleotide reduction, mitochondrial and plasmalemmal membrane permeability, cell viability (apoptosis and necrosis), fluorescent protein labeling and other parameters; 2) intravital microscopy to monitor microcirculation, leukocyte margination, mitochondrial polarization and permeability, radical generation, gene expression and other parameters in living animals; 3) fluorescence and brightfield imaging of immuno-stained tissue samples; 4) fluorescence resonance energy transfer (FRET) to characterize and quantify interactions between specific molecules; 5) Duolink proximity ligation assay to study interactions between endogenously expressed proteins. Ancillary equipment includes a digital darkroom facility for digitizing, analyzing and labeling images; software and computer workstations for 3- and 4-D image analysis and volume rendering; tissue culture hoods and incubators for specimen preparation; and fluorescence and absorbance plate readers for parallel measurements in cultured cells grown on multi-well plates. Imaging core services will promote the success of the individual COBRE projects and will also provide training and assistance to junior investigators studying oxidative stress and stress signaling related to the overall theme of this COBRE. In Phase 1 of the COBRE, CMI Core D contributed to 29 of 44 publications and 26 of 35 grant applications by COBRE investigators.

细胞与分子成像核心D -John Lemasters，医学博士，核心领导者