Development and Application of New Ionization Methods for Biological Mass Spectrometry

生物质谱新型电离方法的开发与应用

• 批准号: 10598032
• 负责人: David C. Muddiman
• 金额: $ 53.35万
• 依托单位: NORTH CAROLINA STATE UNIVERSITY RALEIGH
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-07-01 至 2026-03-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10598032
• 关键词: 3-Dimensional; Ablation; Address; Air; Algorithms; Atmospheric Pressure; Autoimmune Diseases; Biological; Calibration; Cardiometabolic Disease; Charge; Chemicals; Code; Collaborations; Communities; Computer software; Cryoultramicrotomy; Data; Data Analyses; Data Set; Databases; Dedications; Derivation procedure; Development; Disease; Dissociation; Educational workshop; Electrospray Ionization; Engineering; Ensure; Evolution; Fiber Optics; Foundations; Funding; Goals; Health; Heart Diseases; Human; Image; Imaging technology; Informatics; Ischemic Stroke; Isomerism; Lasers; Licensing; Light; Link; Lipids; Malignant Neoplasms; Mass Spectrum Analysis; Measurement; Microscopy; Molecular; Motion; Multimodal Imaging; Neurodegenerative Disorders; Neurotransmitters; Optics; Paper; Pathway interactions; Peptides; Physiological; Play; Polysaccharides; Positioning Attribute; Preparation; Process; Publishing; Recording of previous events; Research; Resolution; Resource Sharing; Role; Sampling; Science; Series; Shapes; Skin; Solid; Solvents; Source; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Spottings; Stroke; Systems Biology; Techniques; Technology; Three-Dimensional Imaging; Time; Tissue imaging; Tissues; Translating; Variant; Vendor; Zebrafish; advanced analytics; analytical tool; biological systems; brain tissue; detection limit; experience; imaging modality; imaging platform; improved; innovation; insight; ionization technique; machine learning algorithm; mass analyzer; mass spectrometer; mass spectrometric imaging; model organism; multidimensional data; neurodevelopment; new technology; next generation; novel; open source; operation; optical fiber; preservation; single cell analysis; software development; technology development; technology platform; whole body imaging; wound healing

PROJECT SUMMARY Mass spectrometry is an extraordinarily powerful bioanalytical technique that has had a profound impact on our molecular understanding of human health and disease. Major advances in mass analyzer technology, dissociation techniques, lasers, and ionization methods are largely attributed to the central role that mass spectrometry plays in the field of systems biology. While mass spectrometry has evolved over the last century into a highly effective analytical tool, there remain significant opportunities for innovation, allowing an even more diverse array of biological questions to be addressed. This proposal is centered on the development of new ionization methods for biological mass spectrometry to enable tissue imaging across several classes of biological molecules. The short- term objective of this proposal is to further develop and fundamentally understand this innovative ionization method using real biological systems. These results will provide a solid foundation from which biological applications will directly benefit. In this mindset, we will develop and apply these new ionization methods to tissue imaging in model organisms to gain mechanistic insights into, 1) ischemic stroke; 2) wound healing; and 3) cardiometabolic disease. The long-term objective is to establish these new ionization methods as an enabling bioanalytical technology to effectively address questions in human health and disease. Public Description of Proposed Research Mass spectrometry (MS), the science related to the “weighing of molecules”, has had a profound impact on the study of human health and disease including cancer, heart disease, neurodegenerative diseases, neural development, and auto-immune diseases. A prerequisite of MS is to convert neutral molecules into charged species (ions) such that they can be “weighed” by the mass spectrometer and identified by advanced analytical techniques. The focus of this research is to develop new ionization methods allowing a more diverse array of contemporary biomedical questions to be addressed. This will include the imaging of tissues to ultimately provide new biological insights into stroke, wound healing and cardiometabolic disease.

项目总结 质谱仪是一种非常强大的生物分析技术，它具有 对我们对人类健康的分子理解产生了深远影响 疾病。质量分析技术、解离技术、 激光和电离方法很大程度上归因于质量的中心作用 光谱学在系统生物学领域发挥作用。而质谱学则有 在上个世纪发展成为一种高效的分析工具，仍然存在 创新的重大机遇，允许更多样化的 需要解决的生物学问题。这项提议的中心是 生物质谱学新电离方法的发展 实现跨几类生物分子的组织成像。简而言之- 这项建议的长期目标是进一步发展和从根本上 理解这种创新的电离方法，使用真实的生物系统。 这些结果将为生物应用提供坚实的基础 将直接受益。本着这种心态，我们将开发和应用这些新的 电离方法在模型生物组织成像中的应用 洞察：1)缺血性中风；2)伤口愈合；3)心脏代谢 疾病。长期目标是建立这些新的电离方法，如 一种能够有效解决人类体内问题的生物分析技术 健康和疾病。 拟议研究的公开说明 质谱学(MS)是一门与“分子重量”相关的科学。 对人类健康和疾病的研究产生了深远的影响，包括 癌症、心脏病、神经退行性疾病、神经发育和 自身免疫性疾病。MS的一个先决条件是将中性分子转化为 带电物种(离子)，使它们能被质量“称重” 并通过先进的分析技术进行鉴定。这件事的重点是 研究是开发新的电离方法，允许更多样化的阵列 需要解决的当代生物医学问题。这将包括 组织成像最终为中风、创伤提供新的生物学见解 治疗和心脏新陈代谢疾病。