The pel exopolysaccharide gene cluster of Pseudomonas aeruginosa
The pel exopolysaccharide gene cluster of Pseudomonas aeruginosa
批准号:
10376298
负责人:
MATTHEW R. PARSEK
金额:
$45.71万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
2009
资助国家:
美国
项目状态:
未结题
起止时间:
2009-07-17 至 2025-03-31
关键词:
AddressBacteriaBacterial AdhesinsCellsChronicClinicalCoculture TechniquesCommunitiesCystic FibrosisDataElementsEnvironmental Risk FactorEventFutureGene ClusterGenotypeGoalsGrowthHumanImmunology procedureIn VitroInfectionKnowledgeLeadLinkMembraneMicrobial BiofilmsMorbidity - disease rateMusMutagenesisMutationNatural ImmunityPathogenesisPathogenicityPersonsPredispositionProductionProteinsPseudomonas aeruginosaPseudomonas aeruginosa infectionRoleSignal TransductionStimulusStressStructureSurfaceSurveysSwimmingSystemTestingTherapeuticUrsidae FamilyWorkWound modelsantimicrobialcell envelopechronic infectionexperimental studyfitnessgenetic approachmortalitymouse modelmutantneutrophilsensortransposon sequencing
中文摘要
摘要
铜绿假单胞菌感染导致人的显著发病率和死亡率
患有囊性纤维化(CF)。这些感染的一个关键特征是它们涉及生物膜
社区.几种关键的生物膜基质组分(例如Pel、Psl)的表达
已知胞外多糖和基质粘附素CdrA)在相互作用后启动
有一个表面。在铜绿假单胞菌中有两种已知的表面传感系统,Wsp和Pil。
Chp,两者都通过刺激细胞内信号c-di-GMP的产生起作用。整体
本申请的目的是调查Wsp表面传感系统,它的信号,
它如何将表面传感与其他表面传感系统Pil-Chp集成
在生物膜聚集体组装的早期阶段。我们还将检查病原体
不同表面传感基因型在感染小鼠模型和感染中的适应性优势
体外细胞免疫学测定。这项建议将填补我们在以下方面的知识空白:
表面传感工作及其对发病机制的贡献。未来的治疗策略
(特别是针对生物膜群落的那些)将受益于这样的知识。
英文摘要
Abstract
Pseudomonas aeruginosa infections result in significant morbidity and mortality in people
suffering from cystic fibrosis (CF). A key feature of these infections is that they involve biofilm
communities. The expression of several key biofilm matrix components (e.g. the Pel,Psl
exopolysaccharides and the matrix adhesin CdrA) are known to be initiated upon interaction
with a surface. There are two known surface sensing systems in P. aeruginosa, Wsp and Pil-
Chp, that both act by stimulating production of the intracellular signal c-di-GMP. The overall
goals of this application are to investigate the Wsp surface sensing system, the signals it
responds to, how it integrates surface sensing with the other surface sensing system Pil-Chp
during the early stages of biofilm aggregate assembly. We will also examine the pathogenic
fitness advantages of different surface sensing genotypes in murine models of infection and in
vitro cellular immunological assays. This proposal will address gaps in our knowledge as to how
surface sensing works and its contribution to pathogenesis. Future therapeutic strategies
(particularly ones directed towards biofilm communities) will benefit from such knowledge.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
Dispersion and the Biofilm Matrix of Pseudomonas aeruginosa
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批准号:10376850
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项目类别:
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资助金额:$55.47万
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财政年份:2021
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负责人:MATTHEW R. PARSEK
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依托单位:
Dispersion and the Biofilm Matrix of Pseudomonas aeruginosa
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批准号:10570255
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项目类别:
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资助金额:$71.31万
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财政年份:2021
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负责人:MATTHEW R. PARSEK
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依托单位:
The pel exopolysaccharide gene cluster of Pseudomonas aeruginosa
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批准号:7653237
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项目类别:
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资助金额:$33.31万
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财政年份:2009
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负责人:MATTHEW R. PARSEK
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依托单位:
The pel exopolysaccharide gene cluster of Pseudomonas aeruginosa
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批准号:8300158
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项目类别:
-
资助金额:$49.39万
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财政年份:2009
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负责人:MATTHEW R. PARSEK
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依托单位:
The pel exopolysaccharide gene cluster of Pseudomonas aeruginosa
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批准号:10609450
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项目类别:
-
资助金额:$45.48万
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财政年份:2009
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负责人:MATTHEW R. PARSEK
-
依托单位:
The pel exopolysaccharide gene cluster of Pseudomonas aeruginosa
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批准号:8757461
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项目类别:
-
资助金额:$45.02万
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财政年份:2009
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负责人:MATTHEW R. PARSEK
-
依托单位:
The pel exopolysaccharide gene cluster of Pseudomonas aeruginosa
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批准号:7893711
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项目类别:
-
资助金额:$50.24万
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财政年份:2009
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负责人:MATTHEW R. PARSEK
-
依托单位:
The pel exopolysaccharide gene cluster of Pseudomonas aeruginosa
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批准号:9102865
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项目类别:
-
资助金额:$48.91万
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财政年份:2009
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负责人:MATTHEW R. PARSEK
-
依托单位:
The pel exopolysaccharide gene cluster of Pseudomonas aeruginosa
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批准号:8102041
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项目类别:
-
资助金额:$49.61万
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财政年份:2009
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负责人:MATTHEW R. PARSEK
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依托单位:
ASM Conference on Biofilms 2003
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批准号:6755809
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项目类别:
-
资助金额:$2.5万
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财政年份:2003
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负责人:MATTHEW R. PARSEK
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依托单位:
AUTOINDUCER SYNTHASE, RH1I
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批准号:2734348
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项目类别:
-
资助金额:$3.15万
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财政年份:1998
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负责人:MATTHEW R. PARSEK
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依托单位:
AUTOINDUCER SYNTHASE, RH1I
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批准号:2412176
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项目类别:
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资助金额:$2.96万
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财政年份:1997
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负责人:MATTHEW R. PARSEK
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依托单位:
国内基金
海外基金
Segmented Filamentous Bacteria激活宿主免疫系统抑制其拮抗菌 Enterobacteriaceae维持菌群平衡及其机制研究
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批准号:81971557
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项目类别:面上项目
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资助金额:65.0万元
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批准年份:2019
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负责人:毛开睿
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依托单位:
电缆细菌(Cable bacteria)对水体沉积物有机污染的响应与调控机制
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批准号:51678163
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项目类别:面上项目
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资助金额:64.0万元
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批准年份:2016
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负责人:许玫英
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依托单位: