National Xenopus Resource Center

项目摘要-核心组件 本部分概述了国家非洲爪哇资源中心提供给社区的各种资源 (NXR)和前五年的详细进展报告。我们概述了该设施的基础设施， 描述了容纳莱维斯和热带X.laevis的两个主要房间以及翻修过的新房间 2017年。我们总结了青蛙的维护、繁殖和向社区分布的情况。在 第二个目标是总结可供社会使用的各种资源和服务，包括 冷冻保存的精子以及用于加强研究的定制转基因和突变资源 非洲爪哇通过促进创建新的蛙线，这在社区中往往是一个障碍，因为 个别实验室无法在自己的机构中培育品系。在第三个目标中，我们描述了NXR如何 通过提供高级培训研讨会、主办PI会议来加强对非洲爪哇的研究 以及它的研究设施服务。所有这些方面都可以通过提供的各种设施 海洋生物实验室，包括教学实验室、成像核心和现场住房。

Project Summary – Core Component This component outlines the various resources available to the community at the National Xenopus Resource (NXR) and a detailed progress report of the previous five years. We outline the infrastructure of the facility, describing the two main rooms housing X. laevis and X. tropicalis as well as the new room that was renovated in 2017. We provide a summary of the maintenance, breeding and distribution of frogs to the community. In the second aim we summarize the various resources and services available to the community, including cryopreserved sperm and the custom transgenesis and mutant resources that serve to enhance research using Xenopus through facilitating the creation of new frog lines that is often an impediment in the community because of the inability of individual labs to breed lines in their own institution. In the third aim, we describe how the NXR enhances novel Xenopus research through its offering of advanced training workshops, hosting of PI meetings and its research facility service. All of these aspects are made possible by the various facilities available at the Marine Biological Laboratory, including teaching labs, imaging core and on-site housing.