National Xenopus Resource Center

项目摘要-核心组件 该部分概述了在国家非洲爪蟾资源中心可供社区使用的各种资源 (NXR)以及过去五年的详细进度报告。我们概述了设施的基础设施， 描述了X的两个主要房间。laevis和X. tropicalis以及新装修的房间 2017年我们提供了一个总结的维护，繁殖和分布的青蛙的社会。在 第二个目标是总结社会上现有的各种资源和服务，包括 冷冻保存的精子和定制的转基因和突变资源，有助于提高研究使用 非洲爪蟾通过促进创造新的青蛙线，这往往是一个障碍，在社会上， 个别实验室无法在自己的机构中繁殖品系。在第三个目标中，我们描述了NXR如何 通过提供高级培训讲习班，主办PI会议， 和研究设施服务。所有这些方面都可以通过在 海洋生物实验室，包括教学实验室、成像核心和现场住房。

Project Summary – Core Component This component outlines the various resources available to the community at the National Xenopus Resource (NXR) and a detailed progress report of the previous five years. We outline the infrastructure of the facility, describing the two main rooms housing X. laevis and X. tropicalis as well as the new room that was renovated in 2017. We provide a summary of the maintenance, breeding and distribution of frogs to the community. In the second aim we summarize the various resources and services available to the community, including cryopreserved sperm and the custom transgenesis and mutant resources that serve to enhance research using Xenopus through facilitating the creation of new frog lines that is often an impediment in the community because of the inability of individual labs to breed lines in their own institution. In the third aim, we describe how the NXR enhances novel Xenopus research through its offering of advanced training workshops, hosting of PI meetings and its research facility service. All of these aspects are made possible by the various facilities available at the Marine Biological Laboratory, including teaching labs, imaging core and on-site housing.