National Xenopus Resource Center

项目总结——核心组件 本部分概述了国家爪蟾资源库中可供社区使用的各种资源 (NXR) 以及过去五年的详细进度报告。我们概述了该设施的基础设施， 描述 X. laevis 和 X.tropicalis 的两个主要房间以及经过翻新的新房间 2017年，我们向社区提供了青蛙的维护、饲养和分配的总结。在 第二个目标我们总结了社区可用的各种资源和服务，包括 冷冻保存的精子以及定制的转基因和突变资源，有助于加强研究 非洲爪蟾通过促进新的青蛙品系的创建，这通常是社区中的一个障碍，因为 个别实验室无法在自己的机构中培育品系。在第三个目标中，我们描述了 NXR 如何 通过提供高级培训研讨会、举办 PI 会议来加强非洲爪蟾的新颖研究 及其研究设施服务。所有这些方面都是通过该中心提供的各种设施来实现的。 海洋生物实验室，包括教学实验室、成像核心和现场住房。

Project Summary – Core Component This component outlines the various resources available to the community at the National Xenopus Resource (NXR) and a detailed progress report of the previous five years. We outline the infrastructure of the facility, describing the two main rooms housing X. laevis and X. tropicalis as well as the new room that was renovated in 2017. We provide a summary of the maintenance, breeding and distribution of frogs to the community. In the second aim we summarize the various resources and services available to the community, including cryopreserved sperm and the custom transgenesis and mutant resources that serve to enhance research using Xenopus through facilitating the creation of new frog lines that is often an impediment in the community because of the inability of individual labs to breed lines in their own institution. In the third aim, we describe how the NXR enhances novel Xenopus research through its offering of advanced training workshops, hosting of PI meetings and its research facility service. All of these aspects are made possible by the various facilities available at the Marine Biological Laboratory, including teaching labs, imaging core and on-site housing.