National Xenopus Resource Center

国家爪蟾资源中心

• 批准号: 10155608
• 负责人: Marko E Horb
• 金额: $ 69.77万
• 依托单位: MARINE BIOLOGICAL LABORATORY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-08-12 至 2025-04-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10155608
• 关键词: Achievement; Address; Amphibia; Animal Housing; Animals; Applied Research; Area; Arts; Back; Bioinformatics; Biological; Biological Models; Biomedical Research; Breeding; Cellular biology; Communities; Complex; Congenital Abnormality; Custom; DNA Sequence Alteration; Development; Diabetes Mellitus; Educational workshop; Ensure; Fertilization in Vitro; Funding; Goals; Grant; Image; Inbreeding; Individual; Institutes; Laboratories; Maintenance; Malignant Neoplasms; Maps; Methods; Neurobiology; Physiology; Productivity; Rana; Regenerative Medicine; Research; Research Personnel; Resources; Scientist; Services; Signal Transduction; Site; System; Techniques; Technology; Training; Transgenes; Transgenic Animals; Transgenic Mice; Transgenic Organisms; United States National Institutes of Health; Universities; Veterinarians; Visit; Wood material; Work; XenBase; Xenopus; Xenopus laevis; animal care; animal resource; biological research; college; genome editing; human disease; insight; integration site; male; meetings; mutant; nervous system disorder; new technology; novel; pathogen; repository; research facility; sperm cryopreservation; training opportunity

Project Summary – Overall Component This project supports continued funding for the National Xenopus Resource (NXR) located at the Marine Biological Laboratory in Woods Hole, MA. The NXR is one of the top priorities for the Xenopus community. The NXR was established in 2010 to serve as a national resource for researchers working with the Xenopus amphibian model system, which includes two species Xenopus laevis and Xenopus tropicalis. The NXR serves as a national repository for community-generated animal stocks as well as serving as an advanced training venue that greatly facilitates productivity for a multitude of researchers. It is a community-oriented resource center that serves all Xenopus researchers, including individuals from large research-focused universities to small liberal arts colleges. As biological research becomes ever more complex, often requiring specialized animal lines and involving diverse technologies, individual laboratory units become insufficient to meet all of the demands required for addressing significant biological problems and a centralized repository or stock center becomes essential. There are four aims to this grant. First, the NXR will maintain current stocks of frogs as well as obtain new lines making them available to the community; this includes special inbred, wild type and mutant lines of both species. In the second aim we outline the NXR research services that meet community demand, including creation of transgenic and mutant animals. In the third aim we outline the resources that we have developed to enable novel Xenopus research, including advanced training workshops (bioinformatics, advanced imaging, genome editing) taught by experts in each field that serve to teach and propagate specialized techniques. The last aim is the applied research aim. In Aim 4 we outline our goals to define the integration sites for our transgenic animals.

项目摘要 - 整体组成部分 该项目支持持续资助位于海军陆战队的国家北欧资源（NXR） 马萨诸塞州伍兹孔的生物实验室。 NXR是Xenopus社区的首要任务之一。这 NXR成立于2010年，是为与Xenopus合作的研究人员提供的国家资源 两栖模型系统，其中包括两个物种Xenopus laevis和xenopus tropicalis。 NXR服务 作为社区生成动物股票的国家存储库，并作为高级培训 为众多研究人员提供了极大的主意生产力的场所。这是一个面向社区的资源 为所有Xenopus研究人员提供服务的中心，包括来自大型研究的大学的个人 小型文科学院。随着生物学研究变得越来越复杂，通常需要专业动物 线条和涉及潜水员技术，个别实验室单位不足以满足所有 解决重大生物问题和集中存储库或库存中心所需的需求 成为必不可少的。这笔赠款有四个目标。首先，NXR还将维持当前的青蛙库存 作为获得新的线路，可以向社区提供它们；这包括特殊的近交，野生型和突变体 两种物种的线条。在第二个目标中，我们概述了满足社区需求的NXR研究服务， 包括创造转基因和突变动物。在第三个目标中，我们概述了我们拥有的资源 开发的旨在启用新颖的Xenopus研究，包括先进的培训研讨会（生物信息学，高级 成像，基因组编辑）由每个领域的专家教授，这些专家用于教授和传播专业 技术。最后的目标是应用研究目标。在目标4中，我们概述了定义集成站点的目标 对于我们的转基因动物。