National Xenopus Resource Center

项目摘要--总体组件 该项目支持继续为位于海军陆战队的国家非洲爪哇资源(NXR)提供资金 马萨诸塞州伍兹霍尔的生物实验室。NXR是非洲爪哇社区的首要任务之一。这个 NXR成立于2010年，是为非洲爪哇研究人员提供的全国性资源 两栖动物模式系统，包括非洲爪哇和热带非洲爪哇两种。NXR提供 作为社区动物种群的国家储存库，以及作为高级培训 极大地促进了众多研究人员的工作效率。它是一种面向社区的资源 为所有非洲爪哇研究人员提供服务的中心，包括从以研究为重点的大型大学到 小型文理学院。随着生物学研究变得越来越复杂，往往需要专门的动物 由于涉及各种不同的技术，单个实验室单位不足以满足所有 解决重大生物问题和集中式存储库或库存中心所需的需求 变得至关重要。这笔赠款有四个目标。首先，NXR还将维持目前青蛙的数量 获得新的品系，使其可供社区使用；这包括特殊的近交系、野生型和突变体 两个物种的品系。在第二个目标中，我们概述了满足社区需求的NXR研究服务， 包括创造转基因和突变动物。在第三个目标中，我们概述了我们拥有的资源 开发以支持新的非洲爪哇研究，包括高级培训讲习班(生物信息学，高级 成像、基因组编辑)由各个领域的专家讲授，这些领域的专家服务于教授和传播专门的 技巧。最后是应用研究的目标。在目标4中，我们概述了定义集成站点的目标 为了我们的转基因动物。

Project Summary – Overall Component This project supports continued funding for the National Xenopus Resource (NXR) located at the Marine Biological Laboratory in Woods Hole, MA. The NXR is one of the top priorities for the Xenopus community. The NXR was established in 2010 to serve as a national resource for researchers working with the Xenopus amphibian model system, which includes two species Xenopus laevis and Xenopus tropicalis. The NXR serves as a national repository for community-generated animal stocks as well as serving as an advanced training venue that greatly facilitates productivity for a multitude of researchers. It is a community-oriented resource center that serves all Xenopus researchers, including individuals from large research-focused universities to small liberal arts colleges. As biological research becomes ever more complex, often requiring specialized animal lines and involving diverse technologies, individual laboratory units become insufficient to meet all of the demands required for addressing significant biological problems and a centralized repository or stock center becomes essential. There are four aims to this grant. First, the NXR will maintain current stocks of frogs as well as obtain new lines making them available to the community; this includes special inbred, wild type and mutant lines of both species. In the second aim we outline the NXR research services that meet community demand, including creation of transgenic and mutant animals. In the third aim we outline the resources that we have developed to enable novel Xenopus research, including advanced training workshops (bioinformatics, advanced imaging, genome editing) taught by experts in each field that serve to teach and propagate specialized techniques. The last aim is the applied research aim. In Aim 4 we outline our goals to define the integration sites for our transgenic animals.