National Xenopus Resource Center

项目概要-总体组成部分 该项目支持继续为位于海洋公园的国家爪蟾资源（NXR）提供资金。 位于马萨诸塞州伍兹霍尔的生物实验室。NXR是Xenopus社区的首要任务之一。的 NXR成立于2010年，作为研究非洲爪蟾的研究人员的国家资源 两栖动物模型系统，其中包括两个物种非洲爪蟾laevis和热带爪蟾。NXR服务于 作为社区产生的动物种群的国家储存库， 场地，极大地促进了生产力的众多研究人员。这是一个面向社区的资源 该中心为所有非洲爪蟾研究人员提供服务，包括来自大型研究型大学的个人， 小型文理学院。随着生物学研究变得越来越复杂，通常需要专门的动物 线和涉及不同的技术，个别实验室单位变得不足以满足所有的 解决重大生物学问题所需的需求和集中的储存库或库存中心 变得至关重要。这笔赠款有四个目的。首先，NXR将维持目前的青蛙库存， 获得新的品系，使它们可用于社区;这包括特殊的近交系，野生型和突变体 两个物种的线。在第二个目标中，我们概述了满足社区需求的NXR研究服务， 包括创造转基因和突变动物。在第三个目标中，我们概述了我们拥有的资源 旨在实现新颖的爪蟾研究，包括高级培训研讨会（生物信息学、高级 成像，基因组编辑），由每个领域的专家教授， 技术.最后是应用研究目标。在目标4中，我们概述了定义集成站点的目标 for our transgenic转基因animals动物.

Project Summary – Overall Component This project supports continued funding for the National Xenopus Resource (NXR) located at the Marine Biological Laboratory in Woods Hole, MA. The NXR is one of the top priorities for the Xenopus community. The NXR was established in 2010 to serve as a national resource for researchers working with the Xenopus amphibian model system, which includes two species Xenopus laevis and Xenopus tropicalis. The NXR serves as a national repository for community-generated animal stocks as well as serving as an advanced training venue that greatly facilitates productivity for a multitude of researchers. It is a community-oriented resource center that serves all Xenopus researchers, including individuals from large research-focused universities to small liberal arts colleges. As biological research becomes ever more complex, often requiring specialized animal lines and involving diverse technologies, individual laboratory units become insufficient to meet all of the demands required for addressing significant biological problems and a centralized repository or stock center becomes essential. There are four aims to this grant. First, the NXR will maintain current stocks of frogs as well as obtain new lines making them available to the community; this includes special inbred, wild type and mutant lines of both species. In the second aim we outline the NXR research services that meet community demand, including creation of transgenic and mutant animals. In the third aim we outline the resources that we have developed to enable novel Xenopus research, including advanced training workshops (bioinformatics, advanced imaging, genome editing) taught by experts in each field that serve to teach and propagate specialized techniques. The last aim is the applied research aim. In Aim 4 we outline our goals to define the integration sites for our transgenic animals.