Center for Cellular Metabolism Research in Oklahoma

俄克拉荷马州细胞代谢研究中心

• 批准号: 10399960
• 负责人: Lijun Xia
• 金额: $ 23.05万
• 依托单位: OKLAHOMA MEDICAL RESEARCH FOUNDATION
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-05-01 至 2022-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10399960
• 关键词: 20 year old; Address; Administrative Supplement; Aging; Animals; Censuses; Centers of Research Excellence; Core Facility; Equipment; Funding; Future; Germ-Free; Growth; Health; Health Sciences; Housing; Institution; Laboratories; Mentors; Metabolism; Mus; Oklahoma; Personal Satisfaction; Population; Reproducibility of Results; Research; Research Personnel; Research Project Grants; Steam; Sterilization; Universities; cost; mouse model; operation; patient derived xenograft model; programs

PROJECT SUMMARY/ABSTRACT OMRF operates three mouse barrier facilities with the only germ-free mouse facility (GFM) and patient-derived xenograft core (PDX) in Oklahoma. The research programs of all five Research Project Leaders (RPLs) on this new COBRE use mouse models, which make up approximately 15% of the daily mouse population of the OMRF mouse facilities. As these investigators grow their research programs in the next five years, this percentage is expected to increase to ~22%. In addition, the laboratories of the CMRO COBRE mentors also utilize mouse models, and the mentors and RPLs combined currently constitute almost 60% of the census. Reliable sterilization of mouse caging and ancillary equipment is essential for mouse barrier facilities, especially those housing immunodeficient and germ-free mice. OMRF’s current sterilizer is 20 years old and is failing. To address this, OMRF plans to replace the failing sterilizer and add a second sterilizer for future expansion. This supplement requests partial support of the sterilizing equipment. OMRF will provide 50% of the total cost for this equipment. The requested equipment is critical for reproducible results for research of the CMRO COBRE RPLs and their mentors as well as animal health and well-being. Additionally, this equipment will also benefit other COBRE and COBRE-eligible investigators from additional institutions in Oklahoma (University of Oklahoma Health Sciences Center and Oklahoma State University), as the GFM and PDX facilities are not found elsewhere in the state.

项目概要/摘要 OMRF 运营着三个小鼠屏障设施，其中包括唯一的无菌小鼠设施 (GFM) 和源自患者的设施 俄克拉荷马州的异种移植核心（PDX）。所有五位研究项目负责人 (RPL) 在此方面的研究计划 新的 COBRE 使用小鼠模型，该模型约占日常小鼠数量的 15% OMRF 鼠标设施。随着这些研究人员在未来五年内扩大他们的研究项目， 预计百分比将增加至约 22%。此外，CMRO COBRE导师的实验室还 利用小鼠模型，目前导师和 RPL 合计占人口普查的近 60%。 老鼠笼和辅助设备的可靠消毒对于老鼠屏障设施至关重要， 尤其是那些饲养有免疫缺陷和无菌小鼠的小鼠。 OMRF 目前的灭菌器已有 20 年历史， 失败。为了解决这个问题，OMRF 计划更换发生故障的灭菌器，并为将来添加第二个灭菌器 扩张。本补充要求灭菌设备的部分支持。 OMRF 将提供 50% 该设备的总成本。所需的设备对于研究的可重复结果至关重要 CMRO COBRE RPL 及其导师以及动物健康和福祉。另外，该设备 俄克拉荷马州其他机构的其他 COBRE 和符合 COBRE 资格的调查人员也将受益 （俄克拉荷马大学健康科学中心和俄克拉荷马州立大学），作为 GFM 和 PDX 该州其他地方找不到设施。