COBRE: OK MED RES FOUND: CORE II: INTRAVITAL MICROSCOPE CORE FACILITY

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 活体显微镜系统代表了一种复杂的工具，用于解剖复杂的生理或病理细胞-细胞或细胞-基质相互作用，例如炎症和动脉粥样硬化中的白细胞募集，免疫发育中的淋巴细胞归巢以及造血中的干细胞归巢。我们的核心设施配备了先进的Nikon ECLIPSE E600-FN活体荧光显微镜， 与Dage-MTI DC-330 3CCD彩色照相机、SVHS盒式录像机和微血管速度OD-RT多普勒装置连接的水浸物镜。该系统还配有一台用于手术准备的Nikon SMZ 800立体显微镜，以及一台用于数据采集和分析的高级戴尔计算机。使用该系统，我们能够以几种方式直接量化循环白细胞与血管内皮的粘附相互作用（即，滚动速度、滚动流量、限定区域中粘附的白细胞的数量）。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The intravital microscopy system represents a sophisticated tool to dissect complex physiological or pathological cell-cell or cell-matrix interactions such as leukocyte recruitment in inflammation and atherosclerosis, lymphocyte homing in immune development, and stem cell homing in hematopoiesis. Our core facility is equipped with an advanced Nikon ECLIPSE E600-FN intravital epifluorescence microscope with water immersion objectives connected to a Dage-MTI DC-330 3CCD color camera, a SVHS video cassette recorder, and a Microvessel Velocity OD-RT Doppler apparatus. The system also has a Nikon SMZ800 stero microscope for surgical preparation, and an advanced Dell computer for data acquisition and analysis. Using the system, we are able to directly quantify adhesive interactions of circulating leukocytes with vascular endothelium in several ways (i.e., rolling velocity, rolling flux, number of adherent leukocytes in a defined area).