Center for Cellular Metabolism Research in Oklahoma

项目总结/摘要 改变的细胞代谢有助于许多常见疾病的发病机制， 俄克拉荷马州。俄克拉荷马州细胞代谢研究中心（CMRO）第一阶段COBRE旨在 在俄克拉荷马州创造和培养卓越的细胞代谢。 目前CMRO COBRE研究项目负责人（RPL）（Lee博士和Cleuren博士）和我们的两名 即将毕业的RPL（Yu博士和Zhao博士）在他们的研究项目中使用共聚焦显微镜。此外，5 CMRO COBRE导师（Xia，Griffin，Olson，Srinivasan和Kovats博士）通常使用共聚焦显微镜 用于他们的研究。2017年购买的尼康C2+共聚焦显微镜，用于支持8名研究人员 在OMRF的心血管生物学研究计划中，目前有13名研究人员使用，其中9名 与COBRE相关者占69.2%。RPL和他们的导师是C2+的最大用户 共焦，并依靠这种设备，以产生一流的图像，为他们的研究，在没有成本。 然而，C2+共焦开始显示出老化和过度使用的证据，其形式是增加 服务呼叫次数和停机时间。此外，较新的共焦显微镜具有许多技术优势。 这些功能在C2+共聚焦中不可用。例如，Nikon AXR显微镜能够 以16倍高的分辨率和2倍高的信号灵敏度扫描2倍大的样品区域。的 AXR还可以以更快的速度采集图像，非常适合更大的样品和实时成像。 此外，AXR能够对采集的图像进行去卷积，并提高分辨率， 进一步.此补充请求部分支持一个新的尼康AXR共焦显微镜。OMRF将 提供设备总成本的50%。所要求的设备将减少对C2+的需求 共聚焦，支持我们的COBRE RPL建立他们的竞争研究计划，并过渡到 独立

PROJECT SUMMARY/ABSTRACT Altered cellular metabolism contributes to the pathogenesis of many common diseases that are prevalent in Oklahoma. This Center for Cellular Metabolism Research in Oklahoma (CMRO) Phase I COBRE aims to create and nurture excellence in cellular metabolism in Oklahoma. Two of the current CMRO COBRE Research Project Leaders (RPLs) (Drs. Lee and Cleuren) and two of our graduating RPLs (Drs. Yu and Zhao) use confocal microscopy for their research programs. Additionally, five CMRO COBRE mentors (Drs. Xia, Griffin, Olson, Srinivasan, and Kovats) routinely use confocal microscope for their research. A Nikon C2+ confocal microscope that was purchased in 2017 to support eight investigators at the Cardiovascular Biology Research Program of OMRF is currently used by thirteen investigators, nine of whom are associated with COBRE (69.2%). The RPLs and their mentors are the heaviest users of the C2+ confocal and have relied on this equipment to produce top-notch images for their research at no cost. However, the C2+ confocal is starting to show evidence of aging and overuse in the form of increasing numbers of service calls and down time. Furthermore, the newer confocal microscopes have many technical capabilities that are not available in the C2+ confocal. For example, the Nikon AXR microscope is capable of scanning 2X larger area of the sample at 16X higher resolution and 2X higher sensitivity to the signal. The AXR can also acquire images at a much faster speed, which is ideal for larger samples and live imaging. Additionally, the AXR is capable of deconvoluting the acquired images and improving the resolution even further. This supplement requests partial support for a new Nikon AXR confocal microscope. OMRF will provide 50% of the total cost for the equipment. The requested equipment will reduce the demand on the C2+ confocal, supporting our COBRE RPLs in building their competitive research programs and transitioning to independence.