Center for Cellular Metabolism Research in Oklahoma

俄克拉荷马州细胞代谢研究中心

• 批准号: 10797920
• 负责人: Lijun Xia
• 金额: $ 25万
• 依托单位: OKLAHOMA MEDICAL RESEARCH FOUNDATION
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-02-05 至 2026-01-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10797920
• 关键词: Administrative Supplement; Aging; Area; Biology; Cardiovascular system; Centers of Research Excellence; Confocal Microscopy; Disease; Equipment; Funding; Image; Mentors; Metabolism; Microscope; Oklahoma; Pathogenesis; Phase; Research; Research Personnel; Research Project Grants; Resolution; Sampling; Scanning; Services; Signal Transduction; Speed; Time; cost; improved; notch protein; programs; success

PROJECT SUMMARY/ABSTRACT Altered cellular metabolism contributes to the pathogenesis of many common diseases that are prevalent in Oklahoma. This Center for Cellular Metabolism Research in Oklahoma (CMRO) Phase I COBRE aims to create and nurture excellence in cellular metabolism in Oklahoma. Two of the current CMRO COBRE Research Project Leaders (RPLs) (Drs. Lee and Cleuren) and two of our graduating RPLs (Drs. Yu and Zhao) use confocal microscopy for their research programs. Additionally, five CMRO COBRE mentors (Drs. Xia, Griffin, Olson, Srinivasan, and Kovats) routinely use confocal microscope for their research. A Nikon C2+ confocal microscope that was purchased in 2017 to support eight investigators at the Cardiovascular Biology Research Program of OMRF is currently used by thirteen investigators, nine of whom are associated with COBRE (69.2%). The RPLs and their mentors are the heaviest users of the C2+ confocal and have relied on this equipment to produce top-notch images for their research at no cost. However, the C2+ confocal is starting to show evidence of aging and overuse in the form of increasing numbers of service calls and down time. Furthermore, the newer confocal microscopes have many technical capabilities that are not available in the C2+ confocal. For example, the Nikon AXR microscope is capable of scanning 2X larger area of the sample at 16X higher resolution and 2X higher sensitivity to the signal. The AXR can also acquire images at a much faster speed, which is ideal for larger samples and live imaging. Additionally, the AXR is capable of deconvoluting the acquired images and improving the resolution even further. This supplement requests partial support for a new Nikon AXR confocal microscope. OMRF will provide 50% of the total cost for the equipment. The requested equipment will reduce the demand on the C2+ confocal, supporting our COBRE RPLs in building their competitive research programs and transitioning to independence.

项目摘要/摘要 细胞代谢改变有助于许多常见疾病的发病机理 俄克拉荷马州。该俄克拉荷马州（CMRO）I期cobre的细胞代谢研究中心旨在 在俄克拉荷马州创造和培养卓越的细胞代谢。 目前的两名CMRO COBRE研究项目负责人（RPLS）（Lee和Cleuren博士）和我们的两个 毕业的RPL（Yu和Zhao博士）使用共聚焦显微镜进行研究计划。另外，五个 CMRO COBRE导师（Xia Dr.Xia，Griffin，Olson，Srinivasan和Kovats）常规使用共聚焦显微镜 为了他们的研究。 2017年购买的尼康C2+共聚焦显微镜 在OMRF的心血管生物学研究计划中，目前有13位调查人员使用 与毛病相关的人（69.2％）。 RPL及其导师是C2+的最重用户 共聚焦并依靠该设备来制作一流的图像，以免费进行研究。 但是，C2+共享以增加形式的衰老和过度使用的证据 服务电话和停机时间的数量。此外，较新的共聚焦显微镜具有许多技术 C2+共聚焦中不可用的功能。例如，尼康AXR显微镜能够 扫描样品的2倍较大区域以16倍的分辨率和对信号的敏感性提高2倍。这 AXR还可以以更快的速度获取图像，这是较大样品和实时成像的理想选择。 此外，AXR能够对获得的图像进行反应并改善分辨率 更远。该补充要求部分支持新的尼康AXR共聚焦显微镜。 OMRF会 提供设备总成本的50％。请求的设备将减少C2+的需求 共焦点，支持我们的毛关系，以构建其竞争研究计划并过渡到 独立。