3rd U.S.-Canada Winter School on Biomolecular Solid State NMR

描述（由申请人提供）：在过去的10-15年中，固态NMR（SSNMR）已经成为一种技术，该技术产生了不适合用X射线衍射或溶液NMR（目前结构生物学的两大支柱）检查的系统的重要结构数据。例如，SSNMR实验最近已经产生了一些膜肽和蛋白质的第一个结构和淀粉样蛋白原纤维的第一个实验确定的结构，这些结构已经抵抗了其他技术的详细研究，因为它们是不溶性的，并且在它们的生物学相关状态下不结晶。这些最近的成功是光谱和样品制备方法的基本和实际改进的结果。与此同时，SSNMR发展中出现的技术，例如平均哈密顿理论，影响了其他研究领域，如溶液NMR和磁共振成像。最后，通过动态核极化（DNP）最初打算SSNMR实验的灵敏度增强的复兴正在发现溶液NMR和成像的广泛应用，并进一步将高场电子顺磁共振（EPR）和NMR集成到一个单一的流形。 为了迅速传播这些进步的科学界，我们建议举行第三届冬季学校的生物分子固态核磁共振斯托，佛蒙特州2013年1月6日至11日。这所学校的主要组成部分将是由该领域的领先研究人员进行的一系列关于基础和高级主题的讲座。课程内容包括现代SSNMR的数学理论基础、特定实验技术的实用优势评估、同位素标记蛋白质和多肽的制备方法、数据处理和数值模拟、从SSNMR数据建立结构模型等。（自旋演化、辛普森、伽马等）目前用于模拟生物固体重要的脉冲NMR实验。参加这所学校将使研究生和博士后学生接触到广泛的基本技术和概念，大大提高了他们通过自己的研究小组的具体研究项目获得的更专业的培训。 这是第三届SSNMR学校，并正在组织响应非常积极的反馈，我们收到的学生和博士后参加了第一和第二学校在2008年1月和2010年。因此，我们认为冬季学校是蓬勃发展的固态生物NMR社区的一个非常重要的教学活动，因此不同于日历上的其他会议。

DESCRIPTION (provided by applicant): In the past 10-15 years, solid state NMR (SSNMR) has emerged as a technique that yields important structural data on systems not amenable to examination with either X-ray diffraction or solution NMR, presently the two pillars of structural biology. For example, SSNMR experiments have recently produced some of the first structures of membrane peptides and proteins and the first experimentally-determined structures of amyloid fibrils, which have been resistant to detailed investigation by other techniques because they are insoluble and do not crystallize in their biologically relevant state. These recent successes are a result of fundamental and practical improvements in spectroscopic and sample preparation methods. Concurrently, technology emerging from developments in SSNMR, for example Average Hamiltonian Theory, has influenced other areas of research such as solution NMR and magnetic resonance imaging. Finally, the renaissance in sensitivity enhancement via dynamic nuclear polarization (DNP) originally intended for SSNMR experiments is finding wide application in solution NMR and imaging and is further integrating high field electron paramagnetic resonance (EPR) and NMR into a single manifold. In order to rapidly propagate these advances to the scientific community we propose to hold the 3rd Winter School on Biomolecular Solid State NMR in Stowe, VT January 6-11, 2013. The principal component of this school will be a series of lectures on fundamental and advanced topics by leading researchers in the field. Topics will include basic aspects of the mathematical theory of modern SSNMR, assessment of practical advantages of specific state-of-the-art experimental techniques, methods for preparation of isotopically labeled proteins and peptides, data processing and numerical simulation, the development of structural models from SSNMR data, etc. The lectures will be supplemented by problem sets and hands-on demonstrations of software (SPINEVOLUTION, SIMPSON, GAMMA, etc.) currently used to simulate pulsed NMR experiments important for biological solids. Attendance at this school will expose graduate and postdoctoral students to a broad spectrum of essential techniques and concepts, greatly enhancing the more specialized training they would otherwise obtain through specific research projects in their own research groups. This is the 3rd SSNMR school and is being organized in response to the very positive feedback that we received from the students and postdoctorals that attended the 1st and 2nd schools in January 2008 and 2010. As a result we believe the Winter School an extremely important pedagogical event for the blossoming solid-state biological NMR community, and as such differs from the plethora of other meetings on the calendar.