800 MHz Low Temperature Magic Angle Spinning NMR Probe.

800 MHz 低温魔角旋转 NMR 探头。

• 批准号: 7794177
• 负责人: ROBERT Guy GRIFFIN
• 金额: $ 30万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-01 至 2011-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7794177
• 关键词: Amyloid Fibrils; Amyloid Proteins; Amyloidosis; Area; Bacteriorhodopsins; Biological; Boston; Communities; Dialysis procedure; Funding Applicant; Future; Gases; Lipid Bilayers; M2 protein; Magic; Membrane; Membrane Proteins; North America; Nuclear; Phosphatidylinositols; Phosphotransferases; Proteins; Research; Research Project Grants; SH3 Domains; Sampling; Scientist; System; Temperature; Vacuum; Vesicle; beta-2 Microglobulin; cold temperature; cryogenics; influenzavirus; innovative technologies; instrument; instrumentation; research study; solid state; solid state nuclear magnetic resonance; structural biology; voltage-dependent anion channel 2; yeast prion

DESCRIPTION (provided by applicant): Funding is requested to support the purchase of a low temperature magic angle spinning (LT-MAS) probe that will be used with a 800/89 solid state spectrometer. The probe includes a cryogenic platform for cooling, transfer lines and the probe itself. The LT-MAS probe operates over the temperature range 90-300 K and will be used to examine a variety of temperature dependent phenomena in cryoprotected membrane protein and amyloid protein samples. The probe has a vacuum dewar, sample eject capabilities, and includes a waveguide for future low temperature dynamic nuclear polarization (DNP) experiments at 527 GHz. Research projects include the following membrane and amyloid proteins: (1) bacteriorhodopsin (bR), (2) the voltage dependent anion channel (VDAC) in lipid bilayers, (3) the M2 protein from influenza virus, (4) gas vesicles which form a proteinaceous membrane, and amyloid fibrils formed from (5) phosphatidyl inositol-3-kinase SH3 domain (PI3-SH3), (6) beta-2-microglobulin associated with dialysis related amyloidosis, and (7) the Sup35p protein, a yeast prion system. The LT-MAS probe on the 800/89 will be part of a cluster of instrumentation that serves a community of ~75 scientist performing high field biological NMR in the Boston area. The instrumentation will alleviate a pressing need for additional capabilities for low temperature MAS experiments. Further, the instrument will have a significant impact on research since it will employ a number of innovative technologies that are not generally available. In addition, the combination of the LT-MAS probe with the 800/89 will be one of the few widebore, low temperature solid state NMR instruments available in North America and will therefore fill an important void in structural biology.

描述（由申请人提供）：请求资金支持购买将与 800/89 固态光谱仪一起使用的低温魔角旋转 (LT-MAS) 探头。该探头包括用于冷却的低温平台、传输线和探头本身。 LT-MAS 探针的工作温度范围为 90-300 K，将用于检查冷冻保护膜蛋白和淀粉样蛋白样品中的各种温度依赖性现象。该探头具有真空杜瓦瓶、样品弹出功能，并包括用于未来 527 GHz 低温动态核极化 (DNP) 实验的波导。研究项目包括以下膜和淀粉样蛋白：(1) 细菌视紫红质 (bR)、(2) 脂质双层中的电压依赖性阴离子通道 (VDAC)、(3) 流感病毒的 M2 蛋白、(4) 形成蛋白质膜的气囊，以及 (5) 磷脂酰形成的淀粉样原纤维 肌醇-3-激酶 SH3 结构域 (PI3-SH3)、(6) 与透析相关淀粉样变性相关的 β-2-微球蛋白，以及 (7) Sup35p 蛋白（一种酵母朊病毒系统）。 800/89 上的 LT-MAS 探头将成为仪器集群的一部分，该仪器集群为波士顿地区约 75 名科学家进行高场生物 NMR 服务。该仪器将缓解低温 MAS 实验对附加功能的迫切需求。此外，该仪器将对研究产生重大影响，因为它将采用许多一般不可用的创新技术。此外，LT-MAS 探头与 800/89 的组合将成为北美为数不多的宽口径低温固态 NMR 仪器之一，因此将填补结构生物学领域的一个重要空白。