Zeiss LSM 980 NLO Confocal Microscope with Airyscan2 for UCI Optical Biology Core

项目摘要/摘要 加州大学欧文分校的光学生物学核心(OBC)请求共享 仪器授权购买蔡司LSM 980非线性光学(NLO)共焦和AiryScan 2胶片 准备好的。LSM 980将带来更高分辨率、更快速的数据采集和AI- 基于用户当前不可用的样本查找。重要的是，LSM 980将取代电流 使用非常频繁且使用年限超过10年的显微镜，因此不再有资格获得服务合同 2022年10月之后的覆盖范围。现有的LSM 780NLO共聚焦显微镜平均使用了超过 过去12个月每月270小时，不包括3个月的大流行病停工，按目前使用量计算 每周将近80个小时。这一旗舰仪器已经成为超过34个国家的研究人员的关键组件 整个园区的实验室，更换它对于保持最高水平的仪器功能至关重要， 在这个广泛的学科范围内的可靠性和可用性。 LSM 980将位于OBC内的专用空间中。OBC将提供管理和 维护显微镜的技术支持以及关于实验设置、培训和成像的建议， 和数据分析专业知识，使用户能够充分利用其功能。OBC的运营时间为 加州大学欧文分校自1987年以来展示了其稳定性、能力和长期的机构承诺 显微镜和成像资源。OBC收到了对仪器服务的强有力的财务承诺 合同、工作人员工资和显微镜配件来自广泛的机构部门，包括 研究室、NCI资助的赵氏家庭综合癌症中心和四所独立学校： 生物科学、工程、医学和物理科学。 替换现有的LSM 780将带来显著改进的成像能力。LSM 980 采用AiryScan探测器(2014年首次推出)，可对高空间频率进行采样 分辨率提高了两倍(120 nm和轴向分辨率350 nm)，最高可提高八倍 改善了信噪比。LSM 980的额外设计和工程改进允许更大的 视场和扫描速度远快于LSM 780，同时继续支持双光子 和荧光寿命成像(FLIM)。这些改进的能力将加强目前使用的研究 LSM 780可在更短的时间内提供更高分辨率的图像。项目对人类健康的具体好处 主要使用者包括更好地了解：(1)癌症和其他疾病的新药疗法如何 疾病可以增强；(2)合成载体输送到细胞；(3)巨噬细胞如何反应 天然和合成生物聚合物；(4)抗生素的发展；(5)组织和神经再生；(6)伤口 修复；以及(7)人类大脑中的突触结构如何受到行为、衰老和记忆的影响。

Project Summary/Abstract The Optical Biology Core (OBC) at the University of California, Irvine (UC Irvine) requests a Shared Instrumentation Grant to purchase a Zeiss LSM 980 Non-Linear Optics (NLO) confocal with Airyscan 2 FLIM ready. The LSM 980 will bring new capabilities such as higher resolution, more rapid data acquisition and AI- based sample finding that are not currently available to users. Importantly, the LSM 980 will replace a current microscope that is very heavily used and is over 10 years old and thus no longer eligible for service contract coverage after October 2022. The existing LSM 780 NLO confocal microscope has been used on average over 270 hours per month for the past 12 months, excluding the 3-month pandemic shutdown, with current usage close to 80 hours per week. This flagship instrument has been a critical component for researchers in over 34 labs across campus, and replacing it is essential to maintaining the highest level of instrument functionality, dependability, and availability across this wide disciplinary range. The LSM 980 will be located in dedicated space within the OBC. The OBC will provide administrative and technical support to maintain the microscope as well as advice on experimental set up, training and imaging, and data analysis expertise to enable users to take full advantage of its capabilities. The OBC has operated at UC Irvine since 1987 demonstrating its stability, capability and a long-standing institutional commitment to microscopy and imaging resources. The OBC receives robust financial commitments towards instrument service contracts, staff salaries and microscope accessories from a broad array of institutional sectors including the Office of Research, the NCI-funded Chao Family Comprehensive Cancer Center and four individual Schools: Biological Sciences, Engineering, Medicine, and Physical Sciences. Replacement of the existing LSM 780 will bring significantly improved imaging capabilities. The LSM 980 incorporates the Airyscan detector (first introduced in 2014), which samples high spatial frequencies with an end result of a two-fold improvement in resolution (120 nm and axial resolution of 350 nm) and up to an eight-fold improvement in signal to noise. Additional design and engineering improvements in the LSM 980 permit a larger field of view and much faster scanning than is possible with the LSM 780 while continuing to support two-photon and Fluorescence Lifetime Imaging (FLIM). These improved capabilities will enhance research currently using the LSM 780 by providing higher-resolution images in less time. Specific benefits to human health from projects of the major users include gaining a better understanding of: (1) how new drug therapies for cancer and other diseases can be enhanced; (2) delivery of synthetic vectors to cells; (3) how macrophages behave in response to natural and synthetic biopolymers; (4) antibiotic development; (5) tissue and nerve regeneration; (6) wound repair; and (7) how synaptic structures in the human brain are affected by behaviors, aging, and memory.