GERM CELL MIGRATION IN DROSOPHILA

果蝇生殖细胞迁移

• 批准号: 2186164
• 负责人: RAHUL WARRIOR
• 金额: $ 17.51万
• 依托单位: UNIVERSITY OF SOUTHERN CALIFORNIA
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-08-01 至 1998-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2186164
• 关键词: Drosophilidae; antiserum; cell cell interaction; cell differentiation; cell migration; complementary DNA; cytogenetics; embryo /fetus cell /tissue; gene complementation; gene expression; gene mutation; genetic mapping; genetic transcription; gonads; histogenesis; in situ hybridization; northern blottings; nucleic acid sequence; phenotype; polymerase chain reaction; transposon /insertion element; western blottings

The long term objective of this research is to learn how groups of cells at different locations in an embryo interact with each other to form a complex structure. This proposal outlines a study of the development and assembly of one such structure, the embryonic gonad of the fruitfly Drosophila . Understanding how the gonad is assembled will require learning more about the mechanism of cell specification, recognition, motility and cell-cell communication. Similar processes of cell specification and migration are characteristic of vertebrate and invertebrate systems both in normal development and in disease such as in the invasiveness and spread of metastatic tumors. This proposal has three specific aims: (I) To determine the role the Drosophila nudC gene plays in germ cell movement and gonad assembly. Drosophila nudC is a novel gene which is expressed in germ cells and is likely to play a role in nuclear or cell motility. The gene was identified using antisera from a patient with the autoimmune disease systemic lupus erythematosus. The gene encodes a protein 50% homologous to a protein required for nuclear movement in the fungus Aspergillus, the nudC gene. (II) To identify the positional cues necessary for pole cell movement and embryonic gonad formation. The assembly of the gonad can be separated into four separate steps each of which is mediated by distinct cues. This specific aim seeks to determine the positional cues required for germ cell movement and the assembly of the embryonic gonad. (III) To identify genes that are involved in the assembly of the gonad by collection of deficiency stocks, The third specific aim seeks to identify novel zygotic genes which play a role in germ cell movement and gonad assembly. This specific aim outlines a screen for zygotic genes using a collection of deletion stocks that cover about two thirds of the genome.

这项研究的长期目标是了解细胞群如何 在胚胎的不同位置， 复杂结构 该提案概述了对发展的研究， 果蝇的胚胎生殖腺：一种这样的结构的集合体， 果蝇了解性腺是如何组装的需要 了解更多关于细胞特化，识别， 运动和细胞间通讯。 细胞相似突起 特化和迁移是脊椎动物特征， 无脊椎动物系统在正常发育和疾病， 转移性肿瘤的侵袭和扩散。 这项建议有三个具体目标： (I)为了确定果蝇nudC基因在生殖细胞中的作用， 运动和性腺组装。果蝇nudC是一个新基因， 在生殖细胞中表达，并可能在细胞核或细胞中发挥作用 能动性 该基因是使用来自一名患有 系统性红斑狼疮 述基因编码 蛋白质50%同源的蛋白质所需的核运动在 真菌曲霉，nudC基因。 (II)为了识别极细胞运动所需的位置线索， 胚胎性腺的形成性腺的组装可以分离 分为四个独立的步骤，每个步骤都由不同的线索介导。 这一特定目标旨在确定所需的位置线索， 生殖细胞的运动和胚胎性腺的组装。 (III)为了鉴定与性腺组装有关的基因 第三个具体目标是， 鉴定在生殖细胞运动中起作用新合子基因， 性腺装配 这个特定的目标概述了合子基因的筛选 使用了一系列的删除股票，涵盖了约三分之二的， 基因组