STRUCTURAL STUDIES OF GRAMICIDIN & OTHER SELF-ASSOCIATING PEPTIDES

短杆菌肽的结构研究

• 批准号: 8361321
• 负责人: MICHAEL L GROSS
• 金额: $ 0.31万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-01-01 至 2011-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361321
• 关键词: Funding; Goals; Gramicidin; Grant; Kinetics; Mass Spectrum Analysis; Measures; Methods; Modeling; National Center for Research Resources; Organic solvent product; Outcome; Peptides; Principal Investigator; Property; Proteins; Reaction; Research; Research Infrastructure; Resources; Solutions; Solvents; Source; United States National Institutes of Health; Vesicle; biomedical resource; cost; dimer; method development; monomer; peptide A; research study

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Our goal is to develop mass spectrometry combined with H/D exchange to study peptide and protein association reactions. Therefore, we chose gramicidin as the subject of our method development of MS methods to study the association of peptides and proteins in solution. We measured the effects of various solvents on the amount of dimer in solution, the outcome of modeling to give the rate constants for the exchange, and the kinetics of monomer/dimer dynamics as revealed by the H/D exchange experiments. We are also comparing the properties of dimeric gramicidin in organic solvents versus in vesicles.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 我们的目标是发展质谱结合H/D交换研究肽和蛋白质缔合反应。因此，我们选择短杆菌肽作为我们MS方法开发的主题，以研究溶液中肽和蛋白质的缔合。 我们测量了各种溶剂对溶液中二聚体的量的影响，建模的结果，以给出交换的速率常数，以及H/D交换实验所揭示的单体/二聚体动力学。 我们还比较了短杆菌肽二聚体在有机溶剂和囊泡中的性质。