APPROACHES TO IMPROVE PROTEIN FOOTPRINTING: HIGH PRESSURE DIGESTION

改善蛋白质足迹的方法：高压消化

• 批准号: 8361405
• 负责人: MICHAEL L GROSS
• 金额: $ 1.57万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-01-01 至 2011-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361405
• 关键词: Digestion; Funding; Grant; Mass Spectrum Analysis; National Center for Research Resources; Peptide Hydrolases; Principal Investigator; Protein Footprinting; Proteins; Research; Research Infrastructure; Resources; Source; System; Technology; United States National Institutes of Health; biomedical resource; cost; improved; pressure

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The incomplete digestion of proteins by proteases is a hindrance in their characterization by mass spectrometry. Some proteins are hard to digest due to aggregation. In order to increase the digestion efficiency of proteases we have developed an on-line high pressure digestion system. This system uses UPLC technology to increase the digestion pressure to a maximum of 10,000 psi.

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 蛋白酶对蛋白质的不完全消化阻碍了蛋白质的质谱学表征。有些蛋白质由于聚集而难以消化。为了提高水解酶的消化效率，我们研制了一套高压在线消化系统。该系统使用UPLC技术，将消化压力提高到最大10,000磅/平方英寸。