HIGH ENERGY COLLISIONAL ACTIVATION

高能碰撞激活

• 批准号: 8361422
• 负责人: MICHAEL L GROSS
• 金额: $ 0.63万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-01-01 至 2011-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361422
• 关键词: Charge; Detection; Financial compensation; Funding; Grant; Ions; Laboratories; Mass Spectrum Analysis; Methods; National Center for Research Resources; Pathway interactions; Performance; Principal Investigator; Proteins; Radial; Research; Research Infrastructure; Resources; Sampling; Source; Staging; Time; United States National Institutes of Health; biomedical resource; cost; design; electric field; improved; instrument; research study

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. In many cases, the need to do multiple stages of MS/MS is removed by the use of high-energy CAD. With low energy activation methods now in use, one or more additional stages of MS/MS may be required because the low ion internal energy results in the sampling of one fragmentation pathway that is non informative. The laboratory frame energies of multiply charged protein ions are calculated to be the order of 10 KeV or more for our 12-T Bruker FTMS instrument. The broad distribution of internal energies that are produced by high-energy CAD result in the sampling of multiple fragmentation pathways and an information rich product ion spectrum. Except in cases such as the quantitation of a targeted molecule, the use of multiple stages of MS/MS to extract information is costly in terms of time and sample. Recent advances in trap design have incorporated the ideas of compensation to improve the detection peformance. All of these efforts seek to remove the detrimental effects of the trapping electric field on detection performance by reshaping the trapping electric field. One of these detrimental effects is the reduction of detection performance for ions that are distributed at high radius that would result from high-energy CAD. Advances in compensation provide a means to remove this limitation that impedes the use of high-energy CAD in the FTMS experiment.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 在许多情况下，通过使用高能量CAD消除了进行MS/MS多个阶段的需要。对于目前使用的低能活化方法，可能需要MS/MS的一个或多个附加阶段，因为低离子内能导致对一个无信息的裂解途径进行采样。 对于我们的12-T Bruker FTMS仪器，计算出多电荷蛋白质离子的实验室框架能量为10 KeV或更高。 由高能CAD产生的内部能量的广泛分布导致对多个碎裂途径的采样和信息丰富的产物离子谱。除了在诸如靶分子的定量的情况下，使用MS/MS的多个阶段来提取信息在时间和样品方面是昂贵的。 在陷阱设计方面的最新进展已经纳入了补偿的思想，以提高检测性能。所有这些努力都试图通过重新整形捕获电场来消除捕获电场对检测性能的不利影响。这些不利影响之一是对于以高半径分布的离子的检测性能的降低，这将由高能量CAD引起。补偿方面的进展提供了一种消除这种限制的方法，这种限制阻碍了在FTMS实验中使用高能CAD。