HIGH ENERGY COLLISIONAL ACTIVATION

高能碰撞激活

• 批准号: 8361422
• 负责人: MICHAEL L GROSS
• 金额: $ 0.63万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-01-01 至 2011-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361422
• 关键词: Charge; Detection; Financial compensation; Funding; Grant; Ions; Laboratories; Mass Spectrum Analysis; Methods; National Center for Research Resources; Pathway interactions; Performance; Principal Investigator; Proteins; Radial; Research; Research Infrastructure; Resources; Sampling; Source; Staging; Time; United States National Institutes of Health; biomedical resource; cost; design; electric field; improved; instrument; research study

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. In many cases, the need to do multiple stages of MS/MS is removed by the use of high-energy CAD. With low energy activation methods now in use, one or more additional stages of MS/MS may be required because the low ion internal energy results in the sampling of one fragmentation pathway that is non informative. The laboratory frame energies of multiply charged protein ions are calculated to be the order of 10 KeV or more for our 12-T Bruker FTMS instrument. The broad distribution of internal energies that are produced by high-energy CAD result in the sampling of multiple fragmentation pathways and an information rich product ion spectrum. Except in cases such as the quantitation of a targeted molecule, the use of multiple stages of MS/MS to extract information is costly in terms of time and sample. Recent advances in trap design have incorporated the ideas of compensation to improve the detection peformance. All of these efforts seek to remove the detrimental effects of the trapping electric field on detection performance by reshaping the trapping electric field. One of these detrimental effects is the reduction of detection performance for ions that are distributed at high radius that would result from high-energy CAD. Advances in compensation provide a means to remove this limitation that impedes the use of high-energy CAD in the FTMS experiment.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 在许多情况下，使用高能量CAD可以消除多个MS/MS的阶段。现在使用低能量激活方法，可能需要一个或多个额外的阶段，因为低离子内部能量会导致一个无用的片段化途径采样。 对于我们的12-T Bruker FTMS仪器，计算出带电蛋白离子的实验室框架能量为10 keV或更多。 高能CAD产生的内部能量的广泛分布导致多个碎片化途径和信息丰富的产物离子光谱采样。除了定量靶向分子之类的情况外，在时间和样本方面，使用多个MS/MS提取信息的阶段是昂贵的。 陷阱设计的最新进展已纳入了补偿的思想，以提高检测的状态。所有这些努力旨在通过重塑捕获电场来消除捕获电场对检测性能的有害影响。这些有害效应之一是在高度半径上分布的离子的检测性能降低，这将导致高能CAD产生。补偿的进步提供了一种消除这种限制的方法，该局限性阻碍了FTMS实验中使用高能CAD的使用。