HIGH ENERGY COLLISIONAL ACTIVATION

高能碰撞激活

• 批准号: 8361422
• 负责人: MICHAEL L GROSS
• 金额: $ 0.63万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-01-01 至 2011-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361422
• 关键词: Charge; Detection; Financial compensation; Funding; Grant; Ions; Laboratories; Mass Spectrum Analysis; Methods; National Center for Research Resources; Pathway interactions; Performance; Principal Investigator; Proteins; Radial; Research; Research Infrastructure; Resources; Sampling; Source; Staging; Time; United States National Institutes of Health; biomedical resource; cost; design; electric field; improved; instrument; research study

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. In many cases, the need to do multiple stages of MS/MS is removed by the use of high-energy CAD. With low energy activation methods now in use, one or more additional stages of MS/MS may be required because the low ion internal energy results in the sampling of one fragmentation pathway that is non informative. The laboratory frame energies of multiply charged protein ions are calculated to be the order of 10 KeV or more for our 12-T Bruker FTMS instrument. The broad distribution of internal energies that are produced by high-energy CAD result in the sampling of multiple fragmentation pathways and an information rich product ion spectrum. Except in cases such as the quantitation of a targeted molecule, the use of multiple stages of MS/MS to extract information is costly in terms of time and sample. Recent advances in trap design have incorporated the ideas of compensation to improve the detection peformance. All of these efforts seek to remove the detrimental effects of the trapping electric field on detection performance by reshaping the trapping electric field. One of these detrimental effects is the reduction of detection performance for ions that are distributed at high radius that would result from high-energy CAD. Advances in compensation provide a means to remove this limitation that impedes the use of high-energy CAD in the FTMS experiment.

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 在许多情况下，通过使用高能CAD消除了进行多个阶段的MS/MS的需要。对于目前使用的低能量激活方法，可能需要一个或多个额外的MS/MS阶段，因为低离子内能导致对一条非信息性碎裂途径的采样。对于我们的12T Bruker FTMS仪器，计算了多电荷蛋白质离子的实验室框架能为10keV或更多的数量级。高能CAD产生的内部能量的广泛分布导致了多条碎裂路径的采样和信息丰富的产物离子光谱。除了目标分子的定量等情况外，使用多个阶段的MS/MS来提取信息在时间和样本方面都是昂贵的。 陷阱设计的最新进展融入了补偿的思想，以提高检测性能。所有这些努力都试图通过重塑陷阱电场来消除陷阱电场对检测性能的不利影响。这些不利影响之一是，由于高能CAD会导致对分布在高半径上的离子的检测性能降低。补偿方面的进展提供了一种方法，可以消除这一限制，阻碍高能CAD在FTMS实验中的使用。