Ambystoma Genetic Stock Center

总体构成部分--项目摘要 这项申请寻求支持，以继续运作的Ambystoma遗传股票中心(AGSC)在 肯塔基大学(英国)。AGSC是唯一一个由联邦政府资助的资源中心，它分发墨西哥语 Aaxolotls(Ambystoma Micianum)，一种提供生物材料支持生物医学的火蜥蜴 国内和国际上的研究。最值得注意的是，轴突在脊椎动物中是独一无二的，因为它能够 再生大量的组织和身体部位。这些器官包括脊髓、四肢、颌骨、视网膜、大脑、心脏、 和尾巴，所有这些都可以在胚胎、幼虫和成年生命的任何时候再生。另外，axolotl是 由从事其他领域工作的研究人员使用，包括神经生物学、生态毒理学、开发、 生理学、细胞生物学、遗传学、基因组学和进化论。对于Axolotl来说，这是一个令人兴奋的时刻，因为 令人难以置信的大基因组(32 GB)最近被测序和组装。新的和可评估的资源包括 允许对基因编辑和单细胞RNA测序等尖端技术进行 第一次，使用axolotl模型快速加速发现的方法。仅在过去的4年里， 美国国立卫生研究院对紫杉醇研究的支持增加了两倍，转基因和敲击试验显著增加。 在社区里划出界线。火蜥蜴社区的PI最近聚在一起讨论未来 机遇和挑战，以及AGSC将在推动社区努力方面发挥的有利作用。 在上一次资助期间，AGSC从文理学院内部迁至 英国医学院(COM)。这一举措在几个重要方面加强了AGSC， 清楚地表明主办机构对这一不可替代资源的长期承诺。具体来说， 提供资金以更换再循环系统，并创建一个更方便用户的网站，以 促进Axolotl股票购买，并更有效地传播对以下工作有用的信息 Axolotls。此外，COM-UK拥有与大学核心研究中心合作的丰富经验 从而提供更稳定的行政环境，以支持AGSC的业务活动，并更好地 确保监管合规以及动物健康和福利。在未来5年，AGSC将在 通过保持和生成增长所需的高质量Axolotl库存来保持当前势头 美国国立卫生研究院资助的调查人员数量。畜牧业和管理做法将得到修改，以增加 研究人员需求不断增加的胚胎后库存的可用性。此外，转基因和 将优先将敲除库存进口到AGSC，并将开发冷冻保存方法以 保存和更高效地管理库存。AGSC将继续作为信息中心，在 调查人员获得有关收藏、技术程序、潜在合作者的信息，以及 研究成果。总体而言，该项目将确保Axolotl资源的分配和长期可持续性 给美国国立卫生研究院资助的调查人员。 好了！

OVERALL COMPONENT - PROJECT SUMMARY This application seeks support to continue operations of the Ambystoma Genetic Stock Center (AGSC) at the University of Kentucky (UK). The AGSC is the only federally funded resource center that distributes Mexican axolotls (Ambystoma mexicanum), a salamander that provides living materials in support of biomedical research nationally and internationally. Most notably, axolotls are unique among vertebrates in being able to regenerate numerous tissues and body parts. These include the spinal cord, limbs, jaw, retina, brain, heart, and tail, all of which can be regenerated at any point during embryonic, larval, and adult life. Also, axolotls are used by researchers that work in additional areas, including neurobiology, ecotoxicology, development, physiology, cell biology, genetics, genomics, and evolution. This is an exciting time for the axolotl as it’s incredibly large genome (32 Gb) was recently sequenced and assembled. New and assessable resources are allowing cutting edge technologies like gene-editing and single cell RNA sequencing to be performed for the first time, approaches that are rapidly accelerating discoveries using axolotl models. In just the past 4 years, NIH support for axolotl research has tripled and there has been a significant increase in transgenic and knock- out lines in the community. PI’s in the salamander community recently came together to discuss future opportunities and challenges, and the enabling role the AGSC will play in moving community efforts forward. During the previous funding period, the AGSC moved internally from the College of Arts and Sciences to the College of Medicine (COM) at UK. This move has strengthened the AGSC in several significant ways that clearly demonstrate long term commitment by the host institution for this irreplaceable resource. Specifically, funding was made available to replace re-circulating systems and create a more user friendly website to facilitate axolotl stock purchasing and more efficiently disseminate information that is useful for working with axolotls. Additionally, COM-UK has extensive experience working with university core research centers and thus provides a more stable administrative environment to support AGSC business activities and to better ensure regulatory compliance and animal health and welfare. Over the next 5 years, the AGSC will build upon current momentum by maintaining and generating high quality, axolotl stocks that are needed by a growing number of NIH-funded investigators. Husbandry and management practices will be modified to increase availability of post-embryonic stocks that are seeing increasing demand by researchers. Also, transgenic and knock-out stocks will be prioritized for import into the AGSC and cryopreservation methods will be developed to preserve and more efficiently manage stocks. The AGSC will continue to serve as an informatics hub where investigators obtain information about the collection, technical procedures, potential collaborators, and research findings. Overall, the project will ensure distribution and long-term sustainability of axolotl resources to NIH-funded investigators. !