Ambystoma Genetic Stock Center

钝口螈遗传资源中心

• 批准号: 10457068
• 负责人: Stephen Randal Voss
• 金额: $ 11.8万
• 依托单位: UNIVERSITY OF KENTUCKY
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-03-01 至 2025-02-28
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10457068
• 关键词: Adolescent; Adult; Ambystoma; Ambystoma mexicanum; Animals; Area; Arts; Awareness; Biological; Biomedical Research; Body part; Brain; Breeding; Businesses; Cellular biology; Collaborations; Collection; Communities; Computers; Cryopreservation; Custom; Development; Disease; Embryo; Embryonic Development; Ensure; Environment; Evolution; Funding; Future; Genes; Genetic; Genome; Genomics; Health; Heart; Hour; Human; IACUC; Informatics; Infrastructure; Institution; International; Jaw; Kentucky; Knock-out; Life; Limb structure; Louisiana; Medicine; Methods; Modeling; Natural regeneration; Neurobiology; Newsletter; Online Systems; Organ Model; Physiology; Play; Population; Practice Management; Procedures; Protocols documentation; Reproducibility; Research; Research Personnel; Research Support; Resources; Retina; Role; Salamander; Science; Shipping; Site; Spinal Cord; System; Tail; Technology; Time; TimeLine; Tissues; Transgenic Organisms; Translating; United States National Institutes of Health; Universities; Vertebrates; Work; animal resource; college; cost; experience; genetic resource; knowledge base; learning strategy; meetings; operation; organ regeneration; preservation; single-cell RNA sequencing; sperm cell; sperm cryopreservation; tissue repair; user-friendly; web site; welfare

OVERALL COMPONENT - PROJECT SUMMARY This application seeks support to continue operations of the Ambystoma Genetic Stock Center (AGSC) at the University of Kentucky (UK). The AGSC is the only federally funded resource center that distributes Mexican axolotls (Ambystoma mexicanum), a salamander that provides living materials in support of biomedical research nationally and internationally. Most notably, axolotls are unique among vertebrates in being able to regenerate numerous tissues and body parts. These include the spinal cord, limbs, jaw, retina, brain, heart, and tail, all of which can be regenerated at any point during embryonic, larval, and adult life. Also, axolotls are used by researchers that work in additional areas, including neurobiology, ecotoxicology, development, physiology, cell biology, genetics, genomics, and evolution. This is an exciting time for the axolotl as it’s incredibly large genome (32 Gb) was recently sequenced and assembled. New and assessable resources are allowing cutting edge technologies like gene-editing and single cell RNA sequencing to be performed for the first time, approaches that are rapidly accelerating discoveries using axolotl models. In just the past 4 years, NIH support for axolotl research has tripled and there has been a significant increase in transgenic and knock- out lines in the community. PI’s in the salamander community recently came together to discuss future opportunities and challenges, and the enabling role the AGSC will play in moving community efforts forward. During the previous funding period, the AGSC moved internally from the College of Arts and Sciences to the College of Medicine (COM) at UK. This move has strengthened the AGSC in several significant ways that clearly demonstrate long term commitment by the host institution for this irreplaceable resource. Specifically, funding was made available to replace re-circulating systems and create a more user friendly website to facilitate axolotl stock purchasing and more efficiently disseminate information that is useful for working with axolotls. Additionally, COM-UK has extensive experience working with university core research centers and thus provides a more stable administrative environment to support AGSC business activities and to better ensure regulatory compliance and animal health and welfare. Over the next 5 years, the AGSC will build upon current momentum by maintaining and generating high quality, axolotl stocks that are needed by a growing number of NIH-funded investigators. Husbandry and management practices will be modified to increase availability of post-embryonic stocks that are seeing increasing demand by researchers. Also, transgenic and knock-out stocks will be prioritized for import into the AGSC and cryopreservation methods will be developed to preserve and more efficiently manage stocks. The AGSC will continue to serve as an informatics hub where investigators obtain information about the collection, technical procedures, potential collaborators, and research findings. Overall, the project will ensure distribution and long-term sustainability of axolotl resources to NIH-funded investigators. !

总体组成部分-项目摘要 该申请寻求支持，以继续运营位于 肯塔基州大学（英国）。AGSC是唯一一个由联邦政府资助的资源中心， 蝾螈（Ambystoma mexicanum），一种蝾螈，提供支持生物医学的生活材料 研究国内和国际。最值得注意的是，蝾螈是脊椎动物中唯一能够 再生许多组织和身体部位。这些包括脊髓，四肢，下巴，视网膜，大脑，心脏， 和尾巴，所有这些都可以在胚胎、幼虫和成虫生命的任何时候再生。同时，蝾螈 研究人员在其他领域工作，包括神经生物学，生态毒理学，发展， 生理学、细胞生物学、遗传学、基因组学和进化。对于美西螈来说这是一个激动人心的时刻 令人难以置信的大基因组（32 Gb）最近被测序和组装。新的和可评估的资源是 允许尖端技术，如基因编辑和单细胞RNA测序，以执行 第一次，使用美西蝾螈模型的方法正在迅速加速发现。在过去的四年里， 美国国立卫生研究院对蝾螈研究的支持增加了两倍，转基因和敲除蝾螈的研究也显著增加。 在社区里排队。火蜥蜴社区的PI最近聚在一起讨论未来 机遇和挑战，以及AGSC在推动社区努力方面将发挥的促进作用。 在上一个资助期间，AGSC内部从艺术和科学学院搬到了 英国医学院（COM）。这一举措在几个重要方面加强了AGSC， 明确表明东道机构对这一不可替代资源的长期承诺。具体地说， 已提供资金更换再循环系统，并创建一个更方便用户的网站， 促进美西蝾螈股票购买和更有效地传播信息，是有用的工作， 蝾螈此外，COM-UK拥有与大学核心研究中心合作的丰富经验， 从而提供了一个更稳定的行政环境，以支持AGSC的业务活动， 确保法规遵从性和动物健康和福利。在未来5年，AGSC将建立在 目前的势头，通过维持和产生高质量的，蝾螈种群，是需要一个不断增长的 美国国立卫生研究院资助的研究人员人数。将修改畜牧业和管理实践， 胚胎后种群的可用性，研究人员的需求越来越大。此外，转基因和 将优先考虑将敲除的库存导入AGSC，并将开发冷冻保存方法， 保护和更有效地管理种群。AGSC将继续作为信息中心， 调查人员获得有关收集、技术程序、潜在合作者的信息， 研究结果。总体而言，该项目将确保美西蝾螈资源的分布和长期可持续性 给NIH资助的研究人员 !