Ambystoma Genetic Stock Center

描述(由申请人提供)：本申请寻求肯塔基大学Ambystoma遗传库中心(AGSC)继续运营的支持。AGSC保留了一批具有历史意义的墨西哥紫杉(Ambystoma Micianum)，这是一种濒危物种，为国内和国际的生物医学研究提供了活的材料。最值得注意的是，研究Axolotl是因为它们在脊椎动物中是独一无二的，能够再生许多组织和身体部位。这些器官包括脊髓、四肢、颌骨、视网膜、大脑、心脏和尾巴，所有这些器官都可以在胚胎、幼年和成年期间的任何位置再生。此外，在许多其他领域工作的研究人员也使用紫杉醇，包括神经生物学、生态毒理学、发育、生理学、细胞生物学、遗传学、基因组学和进化。在接下来的5年里，AGSC将实施新的资源管理计划，以更好地确保Axolotl库存的长期可持续性，并每年朝着更大程度的自给自足迈进。这将通过提供社区所依赖的高质量库存、增加紫杉醇的生产和分销以及提供新的产品和服务来实现。特别是，AGSC将提供显微注射服务，以协助研究人员创造转基因Axolotl。AGSC将继续作为一个信息学中心，调查人员在这里获得关于收集、技术程序、潜在合作者和研究结果的信息。总体而言，该项目将确保Axolotl资源向NIH资助的研究人员的分配和长期可持续性。

DESCRIPTION (provided by applicant): This application seeks support to continue operations of the Ambystoma Genetic Stock Center (AGSC) at the University of Kentucky. The AGSC maintains a historically significant collection of Mexican axolotls (Ambystoma mexicanum), an endangered species that provides living materials in support of biomedical research nationally and internationally. Most notably, axolotls are studied because they are unique among vertebrates in being able to regenerate numerous tissues and body parts. These include the spinal cord, limbs, jaw, retina, brain, heart, and tail, all of which can be regenerated at any poit during embryonic, larval, and adult life. Also, axolotls are used by researchers that work in many additional areas, including neurobiology, ecotoxicology, development, physiology, cell biology, genetics, genomics, and evolution. Over the next 5 years, the AGSC will implement a new resource management plan to better ensure long-term sustainability of axolotl stocks and move each year towards greater self- sufficiency. This will be accomplished by providing high quality stocks that the community has come to depend upon, increasing production and distribution of axolotls, and offering new products and services. In particular, the AGSC will offer a microinjection service to assist investigators in the creation of transgenic axolotls. The AGSC will continue to serve as an informatics hub where investigators obtain information about the collection, technical procedures, potential collaborators, and research findings. Overall, the project will ensure distribution and long-term sustainability of axolotl resources to NIH-funded investigators.