Administrative Supplement: Ambystoma Genetic Stock Center

项目总结 Ambystoma Genetic Stock Center(AGSC)是一个P40研究资源，分布于 实验室无性系(Ambystoma Micianum)给全国的研究人员和教育工作者 在国际上。AGSC容纳了大约880名成年人和数千人 水生循环系统和静态碗中的幼虫/幼体，以支持圈养的野生型 育种群体和越来越多的突变和转基因品系。为了保证身体健康 在AGSC内的紫杉醇及其在生物学实验中的重复性是必要的 遵循严格的畜牧业指导方针。在紫杉醇畜牧业中最关键的变量是水 质量。水质必须极高，以确保紫杉醇的正常发育、生长和 提高生殖能力，消除与病原体有关的疾病风险。美国农业安全委员会 通过确保持续和可读的供水来维持高水质 严格的卫生程序，以确保碗和罐子的清洁。坦克和碗 卫生设施是一个劳动密集型的过程，主要由手工完成，程度较小 使用的是一台过时的商用餐厅洗碗机，它正在迅速失去功能。 此行政副刊将用于购买两台Calypso-Aquatic橱柜垫圈 旨在高效和有效地清洁生物制剂和相关的系统 来自水生生物水槽和碗的生物膜。这些新的洗衣机系统的加入将 提供维持高水质和卫生设施所急需的基础设施 程序，提高紫杉醇饲养效率，并产生健康和可再生的 用于生物医学研究人员的Axolotl系列。

PROJECT SUMMARY The Ambystoma Genetic Stock Center (AGSC) is a P40 Research Resource that distributes laboratory axolotls (Ambystoma mexicanum) to researchers and educators nationally and internationally. The AGSC houses approximately 880 adults and several thousand larvae/juveniles in aquatic recirculating systems and static bowls to sustain a captive, wildtype breeding population and a growing number of mutant and transgenic lines. To ensure the health of axolotls within the AGSC and their reproducibility in biological experiments it is necessary to follow rigorous husbandry guidelines. The most critical variable in axolotl husbandry is water quality. Water quality must be extremely high to ensure normal axolotl development, growth and reproductive capacity, and to eliminate disease risks associated with pathogens. The AGSC maintains high water quality by ensuring a constant and readably available water supply and rigorous sanitary procedures to ensure the cleanliness of bowls and tanks. Tank and bowl sanitation is a labor-intensive process that is primarily done by hand, and to a lesser extent using an outdated, commercial restaurant dishwasher that is rapidly becoming non-functional. This Administrative Supplement will be used to purchase two Calypso - Aquatic Cabinet Washer systems that are designed to efficiently and effectively clean biological agents and associated biofilms from aquatic organism tanks and bowls. The addition of these new washer systems will provide infrastructure that is critically needed to maintain high water quality and sanitary procedures, increase efficiency of axolotl husbandry, and generate healthy and reproducible axolotl lines for biomedical researchers.