Administrative Supplement: Ambystoma Genetic Stock Center

项目摘要 Ambystoma遗传股票中心（AGSC）是一个P40研究资源，分布 实验室蝾螈（Ambystoma mexicanum）的研究人员和教育工作者， 化国际大AGSC容纳大约880名成年人和数千名 水生再循环系统和静态碗中的幼虫/幼体，以维持捕获的野生型 育种群体和越来越多的突变体和转基因品系。为了确保健康 在AGSC内的蝾螈及其在生物学实验中的再现性，有必要 遵循严格的饲养准则。美西蝾螈饲养中最关键的变量是水 质量.水质必须非常高，以确保蝾螈正常发育，生长和繁殖。 生殖能力，并消除与病原体有关的疾病风险。AGSC 通过确保恒定和可读取的供水来保持高水质， 严格的卫生程序，以确保碗和罐的清洁。水箱和碗 卫生是一个劳动密集型的过程，主要靠手工完成， 使用过时的商用餐厅洗碗机，该洗碗机正迅速变得不起作用。 此行政补充将用于购买两台Calypso - Aquatic柜式清洗机 设计用于高效和有效地清洁生物制剂和相关的 来自水生生物槽和碗的生物膜。这些新的洗涤系统的增加将 提供维持高水质和卫生所急需的基础设施 程序，提高蝾螈饲养的效率，并产生健康和可再生的 美西蝾螈的生产线。

PROJECT SUMMARY The Ambystoma Genetic Stock Center (AGSC) is a P40 Research Resource that distributes laboratory axolotls (Ambystoma mexicanum) to researchers and educators nationally and internationally. The AGSC houses approximately 880 adults and several thousand larvae/juveniles in aquatic recirculating systems and static bowls to sustain a captive, wildtype breeding population and a growing number of mutant and transgenic lines. To ensure the health of axolotls within the AGSC and their reproducibility in biological experiments it is necessary to follow rigorous husbandry guidelines. The most critical variable in axolotl husbandry is water quality. Water quality must be extremely high to ensure normal axolotl development, growth and reproductive capacity, and to eliminate disease risks associated with pathogens. The AGSC maintains high water quality by ensuring a constant and readably available water supply and rigorous sanitary procedures to ensure the cleanliness of bowls and tanks. Tank and bowl sanitation is a labor-intensive process that is primarily done by hand, and to a lesser extent using an outdated, commercial restaurant dishwasher that is rapidly becoming non-functional. This Administrative Supplement will be used to purchase two Calypso - Aquatic Cabinet Washer systems that are designed to efficiently and effectively clean biological agents and associated biofilms from aquatic organism tanks and bowls. The addition of these new washer systems will provide infrastructure that is critically needed to maintain high water quality and sanitary procedures, increase efficiency of axolotl husbandry, and generate healthy and reproducible axolotl lines for biomedical researchers.