Ambystoma Genetic Stock Center

项目摘要 Ambystoma遗传股票中心（AGSC）是一个P40研究资源，分布 实验室蝾螈（Ambystoma mexicanum）的研究人员和教育工作者， 化国际大AGSC在水生循环系统中容纳约880名成年人 和静态碗，以维持圈养，野生型繁殖种群和少量的 突变株系和转基因株系。几十个转基因和敲除蝾螈品系 在过去的几年里，美西蝾螈社区创造了更多的线路， 在未来的预期。AGSC没有足够的坦克或基础设施来容纳所有 成年育种者或新创造的品系作为活种群。目前正在努力培育一种精子 冷冻保存方案，使得蝾螈品系可以作为活的原种和 冷冻保存的股票。直到精子冷冻保存方案被开发出来并被证明 为了可重复地允许从冷冻精子中重新衍生美西螈品系，迫切需要 增加AGSC中的坦克数量。本行政补充文件将用于 购买两个再循环系统，将增加240罐的AGSC。添加这些 AGSC的新坦克将提供维持运输线所急需的基础设施， 蝾螈群落

PROJECT SUMMARY The Ambystoma Genetic Stock Center (AGSC) is a P40 Research Resource that distributes laboratory axolotls (Ambystoma mexicanum) to researchers and educators nationally and internationally. The AGSC houses approximately 880 adults in aquatic recirculating systems and static bowls to sustain a captive, wildtype breeding population and a small number of mutant and transgenic lines. Several dozen axolotl transgenic and knock-out axolotl lines have been created by the axolotl community in the past couple of years and more lines are anticipated in the future. The AGSC does not have enough tanks or infrastructure to house all adult breeders or newly created lines as living stocks. An effort is underway to develop a sperm cryopreservation protocol so that axolotl lines can be managed as both living stocks and cryopreserved stocks. Until the sperm cryopreservation protocol is developed and shown to reproducibly allow for the re-derivation of axolotl lines from frozen sperm, there is critical need to increase the number of tanks in the AGSC. This Administrative Supplement will be used to purchase two recirculating systems that will add 240 tanks to the AGSC. The addition of these new tanks to the AGSC will provide infrastructure that is critically needed to sustain lines for the axolotl community.