Ambystoma Genetic Stock Center

项目总结 Ambystoma Genetic Stock Center(AGSC)是一个P40研究资源，分布于 实验室无性系(Ambystoma Micianum)给全国的研究人员和教育工作者 在国际上。AGSC在水生循环系统中容纳了大约880名成年人。 和静态碗，以维持圈养的野生繁殖种群和少量 突变株系和转基因株系。几十个紫杉醇转基因和敲除紫杉醇品系已经 是由axolotl社区在过去几年中创建的，更多的行是 期待在未来。AGSC没有足够的坦克或基础设施来容纳所有人 成年繁殖者或新创造的品系作为活家畜。一项开发精子的努力正在进行中 低温保存协议，使Axolotl系既可以作为活体材料管理，也可以作为活体材料管理 超低温保存的库存。直到开发出精子冷冻保存方案并证明 为了可重复地允许从冷冻精子中重新衍生紫杉醇系，迫切需要 增加AGSC中的坦克数量。本行政副刊将用于 购买两个循环系统，将为AGSC增加240个坦克。再加上这些 AGSC的新坦克将提供维持管道至关重要的基础设施 Aaxolotl社区。

PROJECT SUMMARY The Ambystoma Genetic Stock Center (AGSC) is a P40 Research Resource that distributes laboratory axolotls (Ambystoma mexicanum) to researchers and educators nationally and internationally. The AGSC houses approximately 880 adults in aquatic recirculating systems and static bowls to sustain a captive, wildtype breeding population and a small number of mutant and transgenic lines. Several dozen axolotl transgenic and knock-out axolotl lines have been created by the axolotl community in the past couple of years and more lines are anticipated in the future. The AGSC does not have enough tanks or infrastructure to house all adult breeders or newly created lines as living stocks. An effort is underway to develop a sperm cryopreservation protocol so that axolotl lines can be managed as both living stocks and cryopreserved stocks. Until the sperm cryopreservation protocol is developed and shown to reproducibly allow for the re-derivation of axolotl lines from frozen sperm, there is critical need to increase the number of tanks in the AGSC. This Administrative Supplement will be used to purchase two recirculating systems that will add 240 tanks to the AGSC. The addition of these new tanks to the AGSC will provide infrastructure that is critically needed to sustain lines for the axolotl community.