Ambystoma Genetic Stock Center

钝口螈遗传资源中心

• 批准号: 10609292
• 负责人: Stephen Randal Voss
• 金额: $ 11.77万
• 依托单位: UNIVERSITY OF KENTUCKY
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-07-01 至 2023-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10609292
• 关键词: Address; Administrative Supplement; Ambystoma; Ambystoma mexicanum; Behavioral; Biological; Biological Assay; Biology; Collection; Communities; Courtship; Cryopreservation; Culture Media; Data; Deposition; Derivation procedure; Disease; Embryonic Development; Employee; Female; Fertilization; Fertilization in Vitro; Freezing; Genetic; Harvest; Health; Human; Human Resources; Ice; International; Laboratories; Louisiana; Methods; Modeling; Monitor; Organ Model; Partner in relationship; Process; Protocols documentation; Reagent; Reproducibility; Research; Research Personnel; Resources; Sampling; Spermatids; Spermatogonia; Structure; Time; Training; Translating; Universities; Vas deferens structure; Work; agricultural center; experience; experimental study; genetic resource; male; organ regeneration; repository; sperm cell; sperm cryopreservation; sperm quality; sperm viability; success; tissue repair; undergraduate student

PROJECT SUMMARY The Ambystoma Genetic Stock Center (AGSC) is a P40 Research Resource that distributes laboratory axolotls (Ambystoma mexicanum) to researchers and educators nationally and internationally. The AGSC is currently working on a project to develop a reproducible protocol for cryopreserving axolotl sperm, which is needed to efficiently manage axolotl stocks within the AGSC and the broader axolotl community. Early results from this project found that axolotl spermatids are viable for only a short time after they are packaged into structures called spermatophores. This presents a challenge to develop a sperm cryopreservation protocol because there is a narrow window of time within which spermatophores can be harvested to obtain viable sperm. Additionally, spermatophores were found to be laid by males at various times of the day, more often at night and not during the workday. Temporal parameters for collecting spermatophores with viable sperm have not been defined because the mating process has never been rigorously monitored to know when spermatophores are deposited. Until these parameters are defined, it will not be possible to collect large numbers of viable spermatophores from which to develop and optimize methods for cryopreservation. There is critical need to develop a standard protocol for collecting spermatophores with viable sperm. There is also critical need to optimize and gain capacity for sperm freezing and thawing at the AGSC, and to integrate in vitro fertilization into the workflow. To address these needs, the AGSC will hire a full-time employee with previous experience in cryopreservation and axolotl biology. The new employee will work with undergraduate student workers to monitor axolotl courtship during the day and night for the purpose of recording behavioral data and collecting spermatophores. Spermatophores will be frozen and thawed using reagents and conditions recommended by the Aquatic Germplasm and Genetic Resources Center (AGGRC) at the Louisiana State University Agricultural Center and experiments will be performed to assay sperm viability and in vitro fertilization success. Protocols arising from the proposed work will be made publicly available. This administrative supplement will accelerate progress in developing an axolotl sperm cryopreservation protocol and building AGSC capacity to manage germplasm for the axolotl community. .

项目总结 Ambystoma Genetic Stock Center(AGSC)是一个P40研究资源，分布于 实验室无性系(Ambystoma Micianum)给全国的研究人员和教育工作者 在国际上。AGSC目前正在开展一个项目，以制定一项可重复使用的议定书 用于冷冻保存Axolotl精子，这是有效管理Axolotl内库存所必需的 AGSC和更广泛的Axolotl社区。该项目的早期结果发现，Axolotl 精子细胞在被包装成称为 精原细胞。这对开发精子冷冻保存方案提出了挑战。 因为有一个狭窄的时间窗口，在这段时间内精原细胞可以被收获到 获取有活力的精子。此外，还发现雄性在不同的地方产卵。 白天的时间，更多的时候是在晚上，而不是工作日。的时间参数 用可存活的精子收集精原还没有定义，因为交配 这一过程从来没有受到严格的监控，无法知道精原细胞何时沉积。 在定义这些参数之前，将不可能收集大量可行的 精母细胞，从中发展和优化冷冻保存方法。的确有 迫切需要开发一种标准方案来收集具有存活精子的精原细胞。 还迫切需要优化和提高精子冷冻和解冻的能力 AGSC，并将体外受精纳入工作流程。为了满足这些需求， AGSC将聘请一名具有冷冻保存和Axolotl经验的全职员工 生物学。新员工将与本科生工作人员一起监测Axolotl 白天和晚上的求爱，目的是记录行为数据和收集 精原细胞。使用试剂和条件冷冻和解冻精原细胞 由水产种质和遗传资源中心(AGGRC)在 路易斯安那州立大学农业中心将进行实验，以分析 精子存活率和体外受精成功率。拟议工作产生的议定书将是 向公众开放。这一行政副刊将加快发展进程。 紫杉醇精子冷冻保存方案及AGSC种质管理能力建设 为Axolotl社区。 。