NARMS Cooperative Agreement Program to Strengthen Antibiotic Resistance Surveillance in Retail Food Specimens

项目概要： 明尼苏达州农业部（MDA）一直在与明尼苏达州 卫生部（MDH），CDC和FDA关于零售食品研究的报告， 成立于2002年。该项目旨在确定 在零售肉类中发现的肠道病原体（包括 鸡肉、猪肉、碎牛肉和碎火鸡肉）。在明尼苏达州，MDA和MDH是 对所有肉类进行沙门氏菌检测，对鸡肉和火鸡肉进行弯曲杆菌检测。所有 分离株进一步通过MDA或MDH表征，并发送给FDA进行进一步鉴定。 分析（包括培养确认、抗菌药物敏感性测试和 分子分型）。由2015至2019年，每月共抽取80个肉类样本， 测试（包括猪肉和牛肉）。在这些样本中，所有80个样本都进行了沙门氏菌检测， 所有20个火鸡样本和20个（一半）鸡肉样本都进行了检测， 弯曲杆菌从2020年开始，样本总数下降到每月40个， 已展开海产试验研究。在明尼苏达州，2019年，480只鸡中有63只（13.1%） 240份火鸡肉样品中有35份（14.6%），120份猪肉样品中有15份（12.5%）， 120份碎牛肉样本中有1份（0.8%）沙门氏菌检测呈阳性。虽然可能性不大 明尼苏达州在2019年，480个（9.4%）鸡肉样本中有45个，240个（0.8%）地面 火鸡样本对弯曲杆菌呈阳性反应。MDA和MDH计划继续我们 合作安排。我们将继续从七个县收集样本 明尼阿波利斯-圣保罗大都会区，包括明尼苏达州一半以上的 人口我们还将从2021年开始从农村地区收集。本研究 为我们了解物种、血清型和 在各种零售商店中发现的弯曲杆菌和沙门氏菌的抗菌素耐药性类型 肉。这些数据有助于指导我们的疫情调查， 肉类可能是人类感染的来源。我们遇到了非常 我们在这项研究中遇到了一些障碍或挑战，并认为这是一个 有价值的工具，增加了一个关键组成部分的抗菌素耐药性的图片， 明尼苏达

Project Summary: The Minnesota Department of Agriculture (MDA) has been working with the Minnesota Department of Health (MDH), CDC, and FDA on the Retail Food Study since its inception in 2002. This project was designed to determine the prevalence of antimicrobial resistance among enteric pathogens found in retail meats (including chicken, pork, ground beef, and ground turkey). In Minnesota, MDA and MDH are culturing all meats for Salmonella and chicken and ground turkey for Campylobacter. All isolates are further characterized by MDA or MDH and sent on to FDA for further analysis (including culture confirmation, antimicrobial susceptibility testing, and molecular subtyping). From 2015-2019, a total of 80 meat samples per month were tested (including pork and beef). Among the samples, all 80 were tested for Salmonella, all 20 turkey samples and 20 (half) of the chicken samples were tested for Campylobacter. Starting in 2020 the sample total dropped back to 40 per month and the pilot seafood study was initiated. In Minnesota, in 2019, 63 of 480 (13.1%) chicken samples, 35 of 240 (14.6%) ground turkey samples, 15 of 120 (12.5%) pork samples, and 1 of 120 (0.8%) ground beef samples tested positive for Salmonella. Also, in Minnesota in 2019, 45 of 480 (9.4%) chicken samples and 2 of 240 (0.8%) ground turkey samples tested positive for Campylobacter. MDA and MDH plan to continue our cooperative arrangement. We will continue collect samples from the seven-county Minneapolis-St. Paul metropolitan area, which comprises over half of Minnesota’s population. We will also collect from rural areas starting in 2021. This study has contributed substantial data to our understanding of the species, serotypes, and antimicrobial resistance types of Campylobacter and Salmonella found in various retail meats. This data has been beneficial in guiding our outbreak investigations where meats may have been the source of infection in humans. We have encountered very few barriers or challenges in our experience with this study and feel it has been a valuable tool, adding a critical component to the picture of antimicrobial resistance in Minnesota.