NARMS Cooperative Agreement Program to Strengthen Antibiotic Resistance Surveillance in Retail Food Specimens

项目总结： 明尼苏达州农业部(MDA)一直在与明尼苏达州 卫生部(MDH)、美国疾病控制与预防中心(CDC)和FDA自 成立于2002年。这个项目的目的是为了确定 零售肉类中肠道病原菌的耐药性(包括 鸡肉、猪肉、碎牛肉和碎火鸡)。在明尼苏达州，丙二醛和丙二醛 培养所有肉类中的沙门氏菌，鸡肉和火鸡肉中含有弯曲杆菌。全 分离株的进一步特征是丙二醛或MDH，并被送往FDA进一步 分析(包括培养确认、抗菌药敏感性测试和 分子亚型)。2015-2019年，每月共抽取80份肉类样本 检测(包括猪肉和牛肉)。在这些样本中，80个都进行了沙门氏菌检测， 所有20个火鸡样本和20个(一半)鸡肉样本都进行了检测 弯曲杆菌。从2020年开始，样本总数回落到每月40个， 启动了海鲜试验研究。在明尼苏达州，2019年，480只鸡中有63只(13.1%) 样品，240个火鸡样品中有35个(14.6%)，120个猪肉样品中有15个(12.5%)， 120个碎牛肉样本中有1个(0.8%)对沙门氏菌检测呈阳性。此外，在 明尼苏达州，480个鸡肉样本中有45个(9.4%)，240个鸡肉样本中有2个(0.8%) 火鸡样本的弯曲杆菌检测呈阳性。MDA和MDH计划继续我们的 合作安排。我们将继续从七县采集样本 明尼阿波利斯--圣彼得堡保罗大都市区，占明尼苏达州一半以上 人口。从2021年开始，我们还将从农村地区征收。这项研究有 为我们了解该物种、血清型和 不同零售场所发现的弯曲杆菌和沙门氏菌耐药类型 肉。这些数据有助于指导我们的疫情调查 肉类可能是人类感染源。我们遇到了非常 我们在这项研究中遇到的障碍或挑战很少，我觉得这是一个 有价值的工具，为抗菌素耐药性的图景增加了一个关键成分 明尼苏达州。

Project Summary: The Minnesota Department of Agriculture (MDA) has been working with the Minnesota Department of Health (MDH), CDC, and FDA on the Retail Food Study since its inception in 2002. This project was designed to determine the prevalence of antimicrobial resistance among enteric pathogens found in retail meats (including chicken, pork, ground beef, and ground turkey). In Minnesota, MDA and MDH are culturing all meats for Salmonella and chicken and ground turkey for Campylobacter. All isolates are further characterized by MDA or MDH and sent on to FDA for further analysis (including culture confirmation, antimicrobial susceptibility testing, and molecular subtyping). From 2015-2019, a total of 80 meat samples per month were tested (including pork and beef). Among the samples, all 80 were tested for Salmonella, all 20 turkey samples and 20 (half) of the chicken samples were tested for Campylobacter. Starting in 2020 the sample total dropped back to 40 per month and the pilot seafood study was initiated. In Minnesota, in 2019, 63 of 480 (13.1%) chicken samples, 35 of 240 (14.6%) ground turkey samples, 15 of 120 (12.5%) pork samples, and 1 of 120 (0.8%) ground beef samples tested positive for Salmonella. Also, in Minnesota in 2019, 45 of 480 (9.4%) chicken samples and 2 of 240 (0.8%) ground turkey samples tested positive for Campylobacter. MDA and MDH plan to continue our cooperative arrangement. We will continue collect samples from the seven-county Minneapolis-St. Paul metropolitan area, which comprises over half of Minnesota’s population. We will also collect from rural areas starting in 2021. This study has contributed substantial data to our understanding of the species, serotypes, and antimicrobial resistance types of Campylobacter and Salmonella found in various retail meats. This data has been beneficial in guiding our outbreak investigations where meats may have been the source of infection in humans. We have encountered very few barriers or challenges in our experience with this study and feel it has been a valuable tool, adding a critical component to the picture of antimicrobial resistance in Minnesota.