Veterinary and Colony Management Core

兽医和蜂群管理核心

• 批准号: 10494774
• 负责人: Peter Strick
• 金额: $ 242.64万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-01 至 2027-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10494774
• 关键词: Adherence; Aging; Albumins; Alzheimer' s Disease; Alzheimer' s disease model; Alzheimer' s disease related dementia; Amyloid beta-Protein; Anesthesia procedures; Animal Experimentation; Animal Model; Animals; Autopsy; Biomedical Research; Biopsy Specimen; Blood; Body Weight decreased; Breeding; Callithrix; Callithrix jacchus jacchus; Cardiovascular Diseases; Caring; Cataloging; Cerebrum; Chronic Kidney Failure; Code; Collection; Common Core; Creatinine; Deposition; Diabetes Mellitus; Disease; Embryo Transfer; Enrollment; Ensure; Fibroblasts; Frequencies; Gastrointestinal Diseases; Generations; Haplorhini; Health; Health Promotion; Hematocrit procedure; Hemoglobin; Human; Individual; Infant Care; Insulin Resistance; Liquid substance; Longevity; Longitudinal Studies; Macular degeneration; Malignant Neoplasms; Metabolic dysfunction; Modeling; Monitor; Neurosciences; Obesity; Oocytes; Operative Surgical Procedures; Outcome; Pathologic; Personal Satisfaction; Pregnancy; Primates; Procedures; Records; Recovery; Research Personnel; Research Project Grants; Research Support; Resources; Risk Factors; Sampling; Services; Skin; Study models; Thinness; Tissue Sample; Tissues; Training; Universities; Validation; Veterinarians; Work; age related; aged; alpha synuclein; animal care; biobank; comorbidity; genetic pedigree; gut microbiome; in vivo; nonhuman primate; programs; repository; sample collection; synuclein; welfare

PROJECT SUMMARY VETERINARY AND COLONY MANAGEMENT CORE The common marmoset, Callithrix jacchus, is a small New World non-human primate (NHP) with several practical advantages that make it a valuable resource in biomedical research and neuroscience. Among all NHP species, the marmoset is arguably the most suitable animal model for studies of aging and age-related diseases, including Alzheimer’s disease (AD) and AD-related dementias (ADRD). Marmosets are the shortest-lived anthropoid primates. They have an average lifespan of 12-13 years, and some live past 15 years. This short lifespan allows longitudinal studies in the same individuals over a reasonable period. They are considered “aged” at 8 years. In support of their promise to become the premier NHP model of aging-related disorders, marmosets present age- related pathological changes akin to those observed in humans, including chronic renal disease, cardiovascular disease, diabetes, gastrointestinal disorders, cancer, and cerebral deposits of beta-Amyloid and alpha-synuclein. Notably, the sequence coding Aβ is identical between human and marmoset. Marmosets also display age-related weight loss, reduction in lean mass and calf circumference, macular degeneration, changes in the gut microbiome, and progressive decrease of circulating blood factors such as hemoglobin, hematocrit, albumin, and creatinine. Metabolic dysfunction has been a regular observation in aged, obese marmosets, suggesting that marmosets display an age-dependent increase in insulin resistance similar to humans. All of these co- morbidities in marmosets are relevant to the co-morbidities and risk factors of human AD. The Veterinary and Colony Management Core (VCMC) is an essential Core of the Consortium entitled “Generation, Characterization, and Validation of Marmoset Models of Alzheimer’s Disease (MARMO-AD). The VCMC will work with the Projects and Cores of MARMO-AD to provide consistent, reliable animal care and research support services and promote the health and welfare of all marmosets in the marmoset colony at the University of Pittsburgh. The VCMC comprises a dedicated team of veterinary and husbandry staff that is led by an outstanding veterinarian, who is capable of providing both standard and enhanced animal care. The VCMC breeding program ensures the marmoset colony is self-sufficient and maintain its outbred status. The VCMC will support the MARMO-AD Consortium by providing animal support to the investigators that include the acquisition of both fluid and tissue samples for the Projects and Cores, specialized anesthesia monitoring, surgical support, including oocyte collection and embryo transplantations, and the provision of post-surgical recovery and treatment of marmosets, and the collection of samples during terminal procedures. The breeding program of the VCMC will ensure an ongoing supply of animals for the Investigators of MARMO-AD. Lastly, the VCMC will curate the marmoset biobank repository contents to ensure accurate record-keeping of all tissues and samples collected.

兽医和菌落管理核心项目总结 普通的绒猴（Callithrix jacchus）是一种小型的新世界非人灵长类动物（NHP）， 这些优势使其成为生物医学研究和神经科学的宝贵资源。在所有NHP物种中， 绒猴可以说是研究衰老和年龄相关疾病的最合适的动物模型，包括 阿尔茨海默病（AD）和AD相关性痴呆（ADRD）。绒猴是最短命的灵长类动物 灵长类动物它们的平均寿命为12-13年，有些甚至超过15年。如此短的寿命 在合理的时间内对同一个体进行纵向研究。他们被认为是8岁的“老人”。在 支持他们的承诺，成为首要的NHP模型的衰老相关的疾病，绒猴目前的年龄- 与在人类中观察到的病理变化相似的相关病理变化，包括慢性肾病、心血管疾病、 疾病、糖尿病、胃肠道疾病、癌症和β-淀粉样蛋白和α-突触核蛋白的脑沉积。 值得注意的是，编码Aβ的序列在人类和绒猴之间是相同的。绒猴也表现出与年龄有关的 体重减轻，瘦体重和小腿围减少，黄斑变性，肠道变化 微生物组，以及循环血液因子如血红蛋白，血细胞比容，白蛋白， 和肌酸酐。代谢功能障碍是老年肥胖绒猴的常见现象，这表明 绒猴显示出与人类相似的胰岛素抵抗的年龄依赖性增加。所有这些共同- 绒猴的发病率与人类AD的共病和危险因素有关。兽医和 菌落管理核心（VCMC）是一个重要的核心联盟题为“生成，表征， 和阿尔茨海默病的绒猴模型的验证（MARMO-AD）。VCMC将与项目合作 和MARMO-AD的核心，提供一致，可靠的动物护理和研究支持服务，并促进 匹兹堡大学绒猴群中所有绒猴的健康和福利。关于VCMC 由一名杰出的兽医领导的兽医和畜牧人员组成的专门团队， 能够提供标准和增强的动物护理。VCMC育种计划确保 绒猴群体是自给自足的，并保持其远交状态。VCMC将支持MARMO-AD 通过向研究者提供动物支持（包括采集液体和组织）进行联合 项目和核心样本、专业麻醉监测、手术支持，包括卵母细胞 收集和胚胎移植，并提供手术后恢复和治疗绒猴， 以及在终结程序期间收集样本。VCMC的育种计划将确保 为MARMO-AD研究者持续提供动物。最后，VCMC将管理绒猴 生物库储存库的内容，以确保准确记录所有收集的组织和样本。