Veterinary and Colony Management Core

项目摘要兽医和群体管理核心 常见的绒猴Callithrix Jacchus是一种小型的新大陆非人灵长类(NHP)，具有几种实用的 优势，使其成为生物医学研究和神经科学的宝贵资源。在所有NHP物种中， 可以说，绒猴是研究衰老和与年龄相关的疾病的最合适的动物模型，包括 阿尔茨海默病(AD)和AD相关痴呆(ADRD)。绒猴是最短命的类人猿 灵长类动物。他们的平均寿命为12-13年，有些人的寿命超过了15年。这一短暂的寿命使 在一段合理的时间内对同一个体进行纵向研究。他们被认为在8岁时“变老”了。在……里面 支持他们成为衰老相关疾病的首屈一指的NHP模型的承诺，今天的绒猴- 与人类相似的相关病理变化，包括慢性肾脏疾病、心血管疾病 疾病、糖尿病、胃肠疾病、癌症以及脑内沉积的β-淀粉样蛋白和α-突触核蛋白。 值得注意的是，编码Aβ的序列在人类和绒猴之间是相同的。绒猴也会表现出与年龄相关的 体重减轻，瘦体重和小腿围减少，黄斑变性，肠道变化 微生物组和循环血液因子如血红蛋白、红细胞压积、白蛋白、 还有肌酸酐。代谢功能障碍一直是老年肥胖绒猴的常规观察结果，这表明 与人类类似，绒猴表现出与年龄相关的胰岛素抵抗增加。所有这些合作- 绒猴的发病率与人类阿尔茨海默病的共病和危险因素有关。兽医和 殖民地管理核心(VCMC)是名为“世代、特性、 和阿尔茨海默病Marmoset模型的验证(Marmo-AD)。VCMC将与这些项目合作 和Marmo-AD核心，提供一致、可靠的动物护理和研究支持服务，并促进 匹兹堡大学绒猴群体中所有绒猴的健康和福利。VCMC 由一位杰出的兽医领导的兽医和畜牧工作人员组成的敬业团队，他是 能够提供标准和强化的动物护理。VCMC育种计划确保 绒猴群体是自给自足的，并保持其远交状态。VCMC将支持Marmo-AD 财团通过向调查人员提供动物支持，包括获取液体和组织 项目和核心的样本、专门的麻醉监测、手术支持，包括卵母细胞 采集和胚胎移植，提供手术后恢复和治疗绒猴， 以及在终端程序期间的样本采集。VCMC的育种计划将确保 为Marmo-AD的调查人员持续提供动物。最后，VCMC将管理这只绒猴 生物库内容，以确保准确记录收集的所有组织和样本。

PROJECT SUMMARY VETERINARY AND COLONY MANAGEMENT CORE The common marmoset, Callithrix jacchus, is a small New World non-human primate (NHP) with several practical advantages that make it a valuable resource in biomedical research and neuroscience. Among all NHP species, the marmoset is arguably the most suitable animal model for studies of aging and age-related diseases, including Alzheimer’s disease (AD) and AD-related dementias (ADRD). Marmosets are the shortest-lived anthropoid primates. They have an average lifespan of 12-13 years, and some live past 15 years. This short lifespan allows longitudinal studies in the same individuals over a reasonable period. They are considered “aged” at 8 years. In support of their promise to become the premier NHP model of aging-related disorders, marmosets present age- related pathological changes akin to those observed in humans, including chronic renal disease, cardiovascular disease, diabetes, gastrointestinal disorders, cancer, and cerebral deposits of beta-Amyloid and alpha-synuclein. Notably, the sequence coding Aβ is identical between human and marmoset. Marmosets also display age-related weight loss, reduction in lean mass and calf circumference, macular degeneration, changes in the gut microbiome, and progressive decrease of circulating blood factors such as hemoglobin, hematocrit, albumin, and creatinine. Metabolic dysfunction has been a regular observation in aged, obese marmosets, suggesting that marmosets display an age-dependent increase in insulin resistance similar to humans. All of these co- morbidities in marmosets are relevant to the co-morbidities and risk factors of human AD. The Veterinary and Colony Management Core (VCMC) is an essential Core of the Consortium entitled “Generation, Characterization, and Validation of Marmoset Models of Alzheimer’s Disease (MARMO-AD). The VCMC will work with the Projects and Cores of MARMO-AD to provide consistent, reliable animal care and research support services and promote the health and welfare of all marmosets in the marmoset colony at the University of Pittsburgh. The VCMC comprises a dedicated team of veterinary and husbandry staff that is led by an outstanding veterinarian, who is capable of providing both standard and enhanced animal care. The VCMC breeding program ensures the marmoset colony is self-sufficient and maintain its outbred status. The VCMC will support the MARMO-AD Consortium by providing animal support to the investigators that include the acquisition of both fluid and tissue samples for the Projects and Cores, specialized anesthesia monitoring, surgical support, including oocyte collection and embryo transplantations, and the provision of post-surgical recovery and treatment of marmosets, and the collection of samples during terminal procedures. The breeding program of the VCMC will ensure an ongoing supply of animals for the Investigators of MARMO-AD. Lastly, the VCMC will curate the marmoset biobank repository contents to ensure accurate record-keeping of all tissues and samples collected.