MOTOR CONTROL AND CYTOKINES IN CHRONIC FATIGUE SYNDROME

描述：(改编自调查人员摘要)假设 在这个项目中要测试的是电机系统在 CFS患者细胞因子的产生和反应性。 介于刺激呈现之间的一系列神经事件 CFS患者的运动反应将在相对较低的时间进行研究 休息，并在运动性疲劳后再次休息。神经- 要采取的生理措施包括与感觉事件相关的 潜伏期(即听觉脑干电位)、认知事件- 短时记忆任务中的相关电位与运动前相关 潜力。还需测量运动皮质兴奋性的变化。 在运动前，锥体束和感觉的传导速度- 运动皮质对肌肉传入活动作出反应。细胞因子将是 内毒素刺激的血清和培养上清液的测定 用ELISA法检测黏附细胞和植物血凝素(PHA)刺激的T细胞 和生物检测。细胞因子mR-NA将通过聚合酶图谱进行测量 使用特定探针的链式反应(PCR)。细胞因子受体将是 用聚合酶链式反应和免疫印迹技术在基因水平分析T细胞和巨噬细胞 用单抗免疫印迹法检测蛋白质水平。结果是 应提供CFS疲劳的客观测量，本地化 运动反应性对特定神经部位的改变(S) 定义运动反应变化如何与细胞因子的产生和 细胞因子受体表达。

DESCRIPTION: (Adapted from investigator's abstract) The hypotheses to be tested in this project are that the motor system is altered in patients with CFS along with cytokine production and responsiveness. The sequence of neural events intervening between stimulus presentation and motor response will be studied in patients with CFS when relatively rested and again following exercise-induced fatigue. The neuro- physiological measures to be made include sensory event-related potential (that is, auditory brainstem potentials), cognitive event- related potentials in a short-term memory task and pre- movement-related potentials. Also to be measured are excitability changes of motor cortex prior to movement, conductivity velocity of pyramidal tract and sensory- motor cortex responses to muscle afferent activity. Cytokines will be measured in the serum and the cultured supernatants of LPS-stimulated adherent cells and phytohemagglutinin (PHA)-stimulated T cells by ELISA and bioassays. Cytokine mRNA will be measured by MAPPing with polymerase chain reaction (PCR) using specific probes. Cytokine receptors will be analyzed on T cells and macrophages at the mRNA level with PCR and at the protein level by immunoblot using monoclonal antibodies. The results should provide objective measures of fatigue in CFS, localize the alteration of motor responsiveness to particular neural site(s) and to define how motor response changes correlate with cytokine production and cytokine receptor expression.