MALIGNANT BEHAVIOR AND CELLULAR ANTIGEN EXPRESSION

1.细胞遗传学和生物学研究 I.1浆细胞瘤发生 A)MPC前体细胞的特征。 B)继续研究A-MuLV促进MPC的作用。 C)靶细胞水平的MPC易感性的遗传学。I.2 肿瘤抑制 A)小鼠系统：分析单个ChR介导的抑制效应。 B)人类系统：抑制人类的克隆能力和致瘤性 BL细胞。 二、分子研究 II.1 Myc的功能研究 A)继续研究c-myc对SV40复制的影响：i) 参与SV40刺激的c-myc结构域的确定 复制；ii)鉴定与c-myc相互作用的蛋白(S) 这个系统。 B)SV40-Rb-myc相互作用：i)c-myc能否干扰负性 Rb对SV40复制的影响？Ii)是RB对增长的抑制 C-myc原生性表达导致基因产物丧失？ 二.继续研究与myc相关的蛋白激酶活性。 关于Ig/myc易位的继续研究： MPC中新的15；16和6；12易位的分子分析； 远断点RICS和MPC中myc转录水平的分析。 关于B-myc的继续研究：对SV40系统的影响； 与分化相关的研究。 辅助研究：Ig-myc中Rb或P53是否发生改变 易位携带肿瘤？

1. CYTOGENETIC AND BIOLOGICAL STUDIES I.1 Plasmacytomagenesis a) Characterization of the MPC-precursor cell. b) Continued studies on the MPC-accelerating role of A-MuLV. c) The genetics of MPC susceptibility at the target cell level. I.2 Tumor suppression a) Murine system: Analysis of individual chr mediated suppressor effects. b) Human system: Suppression of clonability and tumorigenicity in human BL cells. II. MOLECULAR STUDIES II.1 Functional studies on myc a) Continued studies on the effect of c-myc on SV40 replication: i) Definition of c-myc domains involved in the stimulation of SV40 replication; ii) Identification of protein(s) that interact with c-myc in this system. b) SV40-Rb-myc interactions: i) Can c-myc interfere with the negative effect of Rb on SV40 replication? ii) Is the growth suppression by the Rb gene product abrogated by constitutive c-myc expression? II.2 Continued studies on a myc associated protein kinase activity. II.3 Continued studies on the Ig/myc translocations: Molecular analysis of the new 15;16 and the 6;12 translocations in MPC; Analysis of myc transcription in RICs and MPCs with distant breakpoints. II.4 Continued studies on B-myc: Effect on the SV40 system; differentiation-related studies. III. AUXILIARY STUDIES: Are Rb or p53 changed in Ig-myc translocation-carrying tumors?