MUCOUS CELL DIFFERENTIATION IN AIRWAY EPITHELIUM

气道上皮中的粘液细胞分化

• 批准号: 2217875
• 负责人: Reen Wu
• 金额: $ 22.05万
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-04-01 至 2000-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2217875
• 关键词: bronchial mucus; cell differentiation; gene expression; genetic library; genetic regulation; genetic transcription; genetic translation; human tissue; immunocytochemistry; in situ hybridization; molecular cloning; mucins; nucleic acid sequence; organ culture; protein biosynthesis; respiratory epithelium; retinoids

DESCRIPTION: This renewal application seeks to investigate how vitamin A regulates mucous cell differentiation in airway epithelium. Two stages of mucous cell differentiation are proposed. In the first stage, cells are unable to differentiate into mucous cells in response to vitamin A. However, they can be transformed into mucin-producing cells by microinjection of RNA from differentiated mucous cells. This first stage is therefore termed the "competent" stage of mucous cell differentiation, and vitamin A can cause immortalized airway epithelial cell lines to become competent. In the second "progressive" stage, vitamin A induces mucous cell differentiation. Dr. Wu has identified one to three genes from progressive stage cells which cause stage 1 cells to express mucins. This proposal seeks to clone and to characterize these genes, to define the level of regulation by vitamin A (i.e., transcription or translation), to elucidate the expression of these genes in airway epithelium, and finally to define the mechanism by which these genes induce mucous cell differentiation.

描述：此更新应用程序旨在研究维生素如何 A 调节气道上皮中的粘液细胞分化。二 提出了粘液细胞分化的阶段。在第一阶段， 细胞不能分化为粘液细胞以响应 维生素A。然而，它们可以转化为产生粘蛋白的细胞 通过显微注射来自分化的粘液细胞的RNA。这第一 因此，该阶段被称为粘液细胞的“感受态”阶段 分化，维生素A可引起气道上皮永生化 细胞系变得有能力。在第二个“进步”阶段， 维生素A诱导粘液细胞分化。吴博士发现 来自进行期细胞的一到三个基因导致第一阶段 细胞表达粘蛋白。该提案旨在克隆并 表征这些基因，以确定维生素的调节水平 A（即转录或翻译），阐明 这些基因位于气道上皮细胞中，最后确定其机制 这些基因通过这些基因诱导粘液细胞分化。