MUCOUS CELL DIFFERENTIATION IN AIRWAY EPITHELIUM

描述：此续订申请旨在调查维他命 A调节呼吸道上皮中黏液细胞的分化。二 提出了粘液细胞分化的阶段。在第一阶段， 细胞不能分化为粘液细胞 然而，它们可以转化为产生粘蛋白的细胞。 通过显微注射来自分化的粘液细胞的RNA。这是第一次 因此，这一阶段被称为粘液细胞的“胜任”阶段 分化，维生素A可导致呼吸道上皮永生化 细胞系变得有能力。在第二个“进步”阶段， 维生素A诱导粘液细胞分化。吴博士已经确认了 来自进展期细胞的一到三个基因，导致第一阶段 表达粘蛋白的细胞。这项提议旨在克隆和 确定这些基因的特征，以确定维生素的调节水平 A(即转录或翻译)，以阐明 这些基因在呼吸道上皮细胞中的表达，并最终确定其作用机制 这些基因通过这些基因诱导粘液细胞分化。

DESCRIPTION: This renewal application seeks to investigate how vitamin A regulates mucous cell differentiation in airway epithelium. Two stages of mucous cell differentiation are proposed. In the first stage, cells are unable to differentiate into mucous cells in response to vitamin A. However, they can be transformed into mucin-producing cells by microinjection of RNA from differentiated mucous cells. This first stage is therefore termed the "competent" stage of mucous cell differentiation, and vitamin A can cause immortalized airway epithelial cell lines to become competent. In the second "progressive" stage, vitamin A induces mucous cell differentiation. Dr. Wu has identified one to three genes from progressive stage cells which cause stage 1 cells to express mucins. This proposal seeks to clone and to characterize these genes, to define the level of regulation by vitamin A (i.e., transcription or translation), to elucidate the expression of these genes in airway epithelium, and finally to define the mechanism by which these genes induce mucous cell differentiation.