ANIMAL FACILITY IMPROVEMENT

动物设施改善

• 批准号: 2283658
• 负责人: Teresa Sylvina
• 金额: $ 67.99万
• 依托单位: SALK INSTITUTE FOR BIOLOGICAL STUDIES
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-09-30 至 1996-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2283658
• 关键词: ANIMAL; FACILITY; IMPROVEMENT

Presently, 60 individual research projects which require the use of laboratory animals are conducted by 30 investigators at The Salk Institute. These projects, with annual direct costs totalling $7,913,459, include studies in cancer research; molecular, developmental, and systems neurobiology; endocrinology; developmental biology and immunology. In 1988, a comprehensive effort was initiated by the Institute to better support the needs of existing and projected animal research projects, to better comply with provisions of the Animal Welfare Act and PHS policies related to laboratory animal care and use, and to enable the Institute to become AAALAC accredited. Over the past three years, significant improvements have been made in both the animal facilities and the veterinary care and animal husbandry programs to achieve these objectives. PHS support will be needed, however, for the following necessary alterations and renovations of the Cancer Research Animal Facility (CRAF) and equipment purchases to bring the overall objectives for the Institute's animal research programs to fruition. 1. Ensure effective and routine sanitization and/or sterilization by installing one new cage and rack washer, one new cage and bottle washer, one new floor loading autoclave and one new chlori-flush station to upgrade the existing cagewashing area; installing new bumper guards to protect exposed wall surfaces; and purchasing new portable stainless steel racks for holding rat and mouse cages. 2. Improve air quality and minimize cross contamination by installing a new main exhaust duct and new exhaust dampers, adjusting the pitch of fan blades and rebalancing the air supply and exhaust to achieve an acceptable number of room air changes/hour and correct air flow patterns. 3. Establish a centrally managed surgery area that meets NIH standards and the needs of investigators by providing staffing and equipping a centrally located surgery area for major survival surgery on rats, cats and monkeys. 4. Minimize the potential for introducing pathogens into established animal colonies by providing a quarantine area with two animal cubicles and 1 HEPA-filtered biological safety cabinet that is adjacent to, but physically separate from, other animal housing rooms; and purchasing five additional HEPA-filtered laminar flow workbenches to ensure maintenance of the Viral-Antibody-Free status of transgenic and immunodeficient mice (radiation chimeras, SCID and nu/nu) when changing sterilized microisolator cages and performing minor experimental procedures. 5. Meet NIH and USDA standards for housing cats and monkeys and enhance their psychological well-being by replacing existing caging with new stainless steel cages.

目前，有 60 个单独的研究项目需要使用 实验室动物由索尔克研究所的 30 名研究人员进行。 这些项目的年度直接成本总计 7,913,459 美元，包括 癌症研究；分子、发育和系统 神经生物学；内分泌学；发育生物学和免疫学。 在 1988年，研究所发起全面努力，以更好地 支持现有和预计的动物研究项目的需求， 更好地遵守动物福利法和PHS政策的规定 与实验动物护理和使用相关，并使研究所能够 获得 AAALAC 认证。 过去三年，重大 动物设施和环境均得到改善 兽医护理和畜牧业计划以实现这些目标。 然而，以下必要的情况将需要 PHS 支持 癌症研究动物设施 (CRAF) 的改建和翻新 和设备采购，以实现研究所的总体目标 动物研究计划取得成果。 1. 确保有效的常规消毒和​​/或灭菌 安装一台新的笼式和架式清洗机、一台新的笼式和洗瓶机， 一台新的落地式高压灭菌器和一台新的氯冲洗站需要升级 现有的网箱清洗区域；安装新的保险杠防护装置以保护 裸露的墙面；并购买新的便携式不锈钢架 用于固定大鼠和小鼠笼子。 2. 通过安装空气净化器来改善空气质量并最大限度地减少交叉污染 新的主排气管和新的排气挡板，调整风扇的螺距 叶片并重新平衡供气和排气以达到可接受的 每小时室内换气次数以及正确的气流模式。 3.建立符合NIH标准的集中管理手术区 通过提供人员配备和装备集中调查人员的需求 为大鼠、猫和猴子进行大型生存手术的定位手术区。 4. 最大限度地减少将病原体引入已建立的环境的可能性 动物群落，提供一个带有两个动物隔间的检疫区和 1 个 HEPA 过滤生物安全柜，毗邻但 与其他动物饲养室物理隔离；并购买五 额外的 HEPA 过滤层流工作台，以确保维护 转基因和免疫缺陷小鼠的无病毒抗体状态 （辐射嵌合体、SCID 和 nu/nu）更换灭菌微隔离器时 笼子并进行小型实验程序。 5. 符合 NIH 和 USDA 饲养猫和猴子的标准，并增强 通过用新的笼子取代现有的笼子来改善他们的心理健康 不锈钢笼。