ANIMAL FACILITY IMPROVEMENT

目前，60个需要使用 实验动物由索尔克研究所的30名研究人员进行。 这些项目的年度直接费用共计7 913 459美元，包括 癌症研究;分子、发育和系统 神经生物学;内分泌学;发育生物学和免疫学。 在 1988年，研究所发起了一项全面的努力， 支持现有和计划中的动物研究项目的需求， 更好地遵守动物福利法和PHS政策的规定 与实验动物护理和使用相关，并使研究所能够 获得AAALAC认证。 在过去的三年里， 在动物设施和 兽医护理和畜牧业计划，以实现这些目标。 但是，以下必要的服务将需要PHS支持： 癌症研究动物设施（Cancer Research Animal Facility，CRAF） 和设备采购，使研究所的总体目标， 动物研究项目取得成果。 1. 通过以下方式确保有效和常规的消毒和/或灭菌： 安装一个新的笼子和机架清洗机，一个新的笼子和瓶子清洗机， 一个新的地板装载高压灭菌器和一个新的氯化物冲洗站需要升级 现有的笼子清洗区;安装新的保险杠， 裸露的墙面;以及购买新的便携式不锈钢衣架 用来装老鼠的笼子 2. 改善空气质量，减少交叉污染， 新的主排气管和新的排气挡板，调整风扇的螺距 叶片和重新平衡空气供应和排气，以实现可接受的 每小时室内换气次数和正确的气流模式。 3. 建立一个符合NIH标准的集中管理的手术区， 为满足调查人员的需要，提供人员配置和设备， 位于手术区，用于大鼠、猫和猴子的重大生存手术。 4. 最大限度地减少将病原体引入已建立的 动物殖民地，提供两个动物隔离区， 1个HEPA过滤的生物安全柜，靠近，但 与其他动物饲养室物理分离;并购买五个 额外的HEPA过滤层流工作台，以确保维护 转基因和免疫缺陷小鼠无病毒抗体状态 （辐射嵌合体、SCID和nu/nu）更换灭菌微隔离器时 笼和进行小实验程序。 5. 符合NIH和USDA关于猫和猴的饲养标准， 他们的心理健康，取代现有的笼子与新的 不锈钢笼。

Presently, 60 individual research projects which require the use of laboratory animals are conducted by 30 investigators at The Salk Institute. These projects, with annual direct costs totalling $7,913,459, include studies in cancer research; molecular, developmental, and systems neurobiology; endocrinology; developmental biology and immunology. In 1988, a comprehensive effort was initiated by the Institute to better support the needs of existing and projected animal research projects, to better comply with provisions of the Animal Welfare Act and PHS policies related to laboratory animal care and use, and to enable the Institute to become AAALAC accredited. Over the past three years, significant improvements have been made in both the animal facilities and the veterinary care and animal husbandry programs to achieve these objectives. PHS support will be needed, however, for the following necessary alterations and renovations of the Cancer Research Animal Facility (CRAF) and equipment purchases to bring the overall objectives for the Institute's animal research programs to fruition. 1. Ensure effective and routine sanitization and/or sterilization by installing one new cage and rack washer, one new cage and bottle washer, one new floor loading autoclave and one new chlori-flush station to upgrade the existing cagewashing area; installing new bumper guards to protect exposed wall surfaces; and purchasing new portable stainless steel racks for holding rat and mouse cages. 2. Improve air quality and minimize cross contamination by installing a new main exhaust duct and new exhaust dampers, adjusting the pitch of fan blades and rebalancing the air supply and exhaust to achieve an acceptable number of room air changes/hour and correct air flow patterns. 3. Establish a centrally managed surgery area that meets NIH standards and the needs of investigators by providing staffing and equipping a centrally located surgery area for major survival surgery on rats, cats and monkeys. 4. Minimize the potential for introducing pathogens into established animal colonies by providing a quarantine area with two animal cubicles and 1 HEPA-filtered biological safety cabinet that is adjacent to, but physically separate from, other animal housing rooms; and purchasing five additional HEPA-filtered laminar flow workbenches to ensure maintenance of the Viral-Antibody-Free status of transgenic and immunodeficient mice (radiation chimeras, SCID and nu/nu) when changing sterilized microisolator cages and performing minor experimental procedures. 5. Meet NIH and USDA standards for housing cats and monkeys and enhance their psychological well-being by replacing existing caging with new stainless steel cages.