SOLID TUMOR CHROMOSOME ANALYSIS

实体瘤染色体分析

• 批准号: 3633025
• 负责人: AVERY A SANDBERG
• 金额: $ 4.15万
• 依托单位: ROSWELL PARK CANCER INSTITUTE
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-06-30 至 1992-06-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3633025
• 关键词: cancer risk; chromosome disorders; human tissue; karyotype; neoplasm /cancer genetics; neoplastic cell; stainings

This laboratory will be sent fresh solid tumor specimens which they will then disaggregate and incubate under appropriate growth conditions. The laboratory personnel will then make slides from mitotically active cultures. When metaphases have been identified under the microscope, the chromosomes in them will be stained by suitable methods (primarily with Wright's stain, giemsatrypsin or quinacrine) and the individual cells will be analyzed to determine the karyotype. Photographs will be taken of good metaphase spreads, and the chromosomes in them will be studied in detail. The goal of the contract is to identify changes in chromosome number and/or structure that may be important in the etiology of specific types of solid tumors.

本实验室将送来新鲜的实体肿瘤标本 然后，它们将解聚并在适当的生长条件下孵化 条件。然后实验室工作人员将制作幻灯片 有丝分裂活跃的培养物。当中期已经 在显微镜下鉴定，其中的染色体将是 用适当的方法染色(主要是用莱特氏染色， GIEMSARYPIN或QUAKRINE)，单个细胞将被 进行分析以确定核型。会拍一些照片 好的中期传播，其中的染色体将是 进行了详细的研究。合同的目标是确定更改 在染色体数目和/或结构上可能是重要的 特定类型实体肿瘤的病因学。