DROSOPHILA & TUBULIN RING COMPLEX (ANDTURC)

果蝇

• 批准号: 2383451
• 负责人: Yixian Zheng
• 金额: $ 20.87万
• 依托单位: CARNEGIE INSTITUTION OF WASHINGTON, D.C.
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-09-30 至 2002-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2383451
• 关键词: cell cycle; centrosome; electron microscopy; microtubule associated protein; microtubules; molecular cloning; protein structure function

DESCRIPTION: The long-term goal of this research is to understand how centrosomes in animal cells nucleate microtubules and how this nucleating activity is regulated by cell-cycle and developmental processes. The protein complex to be used in this study is the recently identified gamma-tubulin ring complex (gamma-TuRC) which can nucleate and bind to the minus ends of tits nucleated microtubules in vitro. Based on a number of lines of evidence it is hypothesized that the gamma-TuRC is the major microtubule nucleator at the centrosomes. The gamma-TuRC consists of gamma tubulin and at least five currently unidentified proteins (p183, p151, p98, p82 and p75s). The four aims of this proposal are to: 1) clone and biochemically characterize the currently unknown proteins in the gamma-TuRC; 2) elucidate the structural organization of gamma-TuRC; 3)reconstitute functional gamma TuRC in vitro; and 4)determine the detailed functions of the gamma-TuRC.

描述：这项研究的长期目标是了解 动物细胞中的中心体使微管成核及其成核方式 活性受细胞周期和发育过程的调节。这个 在这项研究中使用的蛋白质复合体是最近确定的 伽马-微管蛋白环复合体(伽马-TURC)，可以成核并结合到 山雀的负端在体外可形成微管。基于多个 一连串的证据推测伽马火山就是 中心体上的微管核器。伽马-TURC由伽马组成 微管蛋白和至少五种目前未知的蛋白质(P183，P151，P98， P82和p75s)。这项提议的四个目标是：1)克隆和 对伽马-TURC中目前未知的蛋白质进行生物化学表征； 2)阐明了γ-TURC的结构组织；3)重组 功能伽玛刀的体外实验；4)确定其具体功能 伽马图鲁克。