AIRWAYS CENTRAL NERVOUS SYSTEM CONTROL

这项调查包括两条主要的研究路线，重点是 同样的问题：分析中枢神经系统对气道的控制。第一部分 本基金申请的第一部分涉及中央气道的神经解剖学 第二部分是确定功能的作用 一些特定的中枢神经系统区域在分泌运动的调节中起作用， 支气管炎控制。 解剖学研究将使用伪狂犬病病毒作为跨神经元标记物 研究控制副交感神经流出的起源细胞， 胸内气道（支气管和气道的最远端部分） 以及气管。神经支配的潜在差异 将分析气管支气管系统。详细研究了 支配气管的含神经肽的运动前神经元 副交感神经节前神经元将使用 免疫组织化学方法来识别病毒感染的神经元 和假定的神经递质。最后，化学成分 气管副交感神经节前神经元将使用 胆碱乙酰转移酶和神经肽免疫化学联合 霍乱毒素逆行细胞体标记。 在第二部分中，分泌运动和支气管功能将被 在特定脑核团的L-谷氨酸刺激后研究 已知投射到气道副交感神经节前神经元。 此外，还将研究它们在反射反应中的作用。第一 项目将涉及中线核的分析，特别是 注意力集中在苍白中缝及其邻近区域。第二 该项目涉及对Kolliker-Ehrman核及其周围环境的分析。 地区第三个项目将分析从 背侧脑室周围灰质第四个项目将分析 兴奋性氨基酸在产生副交感神经活动和 介导分泌运动和支气管反射反应。 从这些研究中获得的数据将有助于更好地了解 中枢神经系统影响气道功能， 这与理解中枢介导的气道功能障碍有关。

This investigation consists of two major lines of research focused on the same problem: the analysis of CNS control of the airways. The first part of this grant application deals with neuroanatomy of central airway pathways and the second part is focused on determining the functional role some of the specific CNS regions play in regulation of secretomotor and bronchomotor control. Anatomical studies will use pseudorabies virus as a transneuronal marker to study the cells of origin which control the parasympathetic outflow to the intrathoracic airways (bronchus and distal most part of the airways) as well as the trachea. Potential differences in innervation of the tracheobronchial system will be analyzed. A detailed study of the neuropeptide-containing premotor neurons innervating the tracheal parasympathetic preganglionic neurons will be made using immunohistochemical procedures to identify both virally infected neurons and putative neurotransmitters. Finally, the chemical content of the tracheal parasympathetic preganglionic neurons will be examined using choline acetyltransferase and neuropeptide immunochemistry combined with cholera-toxin retrograde cell body labeling. In the second part, secretomotor and bronchomotor functions will be investigated following L-glutamate stimulation of specific brain nuclei known to project to airway parasympathetic preganglionic neurons. Furthermore, their role in reflex responses will be studied. The first project will involve an analysis of the midline nuclei, with particular attention focused on the raphe pallidus and adjoining region. The second project involves an analysis of the Kolliker-Fuse nucleus and surrounding region. The third project will analyze the responses elicited from the dorsal periventricular gray matter. A fourth project will analyze the role of excitatory amino acids in generation of parasympathetic activity and in mediating secretomotor and bronchomotor reflex responses. The data derived from these studies will provide a better understanding of the CNS neural systems that influence airway function and have a direct bearing on understanding centrally mediated airways dysfunctions.