ADIPOSE TISSUE LIPOLYSIS IN SPINAL CORD INJURED SUBJECTS MEASURED BY MICRODIALYS

通过微透析测量脊髓损伤受试者的脂肪组织脂肪分解

• 批准号: 2441386
• 负责人: R PRATLEY
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2441386
• 关键词: adipose tissue; adrenal medulla; basal metabolism; clinical research; exercise; hormone regulation /control mechanism; human subject; innervation; isoproterenol; lipolysis; microdialysis; spinal cord injury; sympathetic nervous system

The extent to which the sympathetic nervous system and other neuroendocrine transmitters regulate adipose tissue lipolysis during basal and stimulated conditions is unclear. Individuals with a spinal cord injury (SCI) lack direct sympathetic innervation to tissues below the level of lesion. This disruption of neural pathways affects both direct stimulation of abdominal fat tissue as well as indirect stimulation by hormones (i.e., catecholamines) of the adrenal medulla. Because of the lack of direct neural and circulating catecholamine input to lower-body fat tissue, persons with SCI represent a model for examining lipolysis in the absence of major sympathetic drive. This protocol examines the effects of exercise and isoproterenol stimulation on adipose tissue lipolysis in persons with and without sympathetic innervation of the adrenal medulla and adipose tissue. To test the role of direct sympathetic innervation and circulating catecholamines in regulating fat breakdown in adipose tissue, lipolysis (i.e., glycerol production) will be measured in situ in able-bodied subjects and those with a spinal cord injury, at rest and during arm exercise, using the technique of microdialysis. We hypothesize that SCI will have a reduced lipolytic rate, due to lower direct and circulating catecholamine levels, compared to AB subjects. Furthermore, the degree of disruption of lipolysis in SCI will vary according to lesion level, such that subjects with high thoracic (T1- T4) or cervical spinal cord lesions will show the greatest reduction in lipolytic rate, because of loss of both direct and adrenally- mediated sympathetic stimulation.

交感神经系统和其他神经系统的作用程度 神经内分泌递质调节脂肪组织的脂肪分解 基础条件和刺激条件尚不清楚。 有脊椎病的人 脊髓损伤 (SCI) 缺乏对下方组织的直接交感神经支配 病变程度。 这种神经通路的破坏会影响两者 直接刺激腹部脂肪组织以及间接刺激腹部脂肪组织 肾上腺激素（即儿茶酚胺）的刺激 髓质。 由于缺乏直接的神经和循环 儿茶酚胺输入下半身脂肪组织，SCI 患者 代表在没有主要因素的情况下检查脂肪分解的模型 同情的驱动力。 该协议检查运动的效果和 异丙肾上腺素刺激脂肪组织脂肪分解 并且没有肾上腺髓质和脂肪的交感神经支配 组织。测试直接交感神经支配的作用和 循环儿茶酚胺调节脂肪分解 组织，脂肪分解（即甘油的产生）将在原位测量 对于身体健全的受试者和脊髓损伤的受试者，在休息时 在手臂锻炼过程中，使用微透析技术。 我们 假设 SCI 的脂肪分解率会降低，因为 与 AB 受试者相比，直接和循环儿茶酚胺水平。 此外，SCI 中脂肪分解的破坏程度会有所不同 根据病变程度，高胸椎（T1- T4)或颈髓病变会表现出最大程度的减少 脂肪分解率，因为直接和肾上腺的损失 介导的交感神经刺激。