ADIPOSE TISSUE LIPOLYSIS IN SPINAL CORD INJURED SUBJECTS MEASURED BY MICRODIALYS

通过微透析测量脊髓损伤受试者的脂肪组织脂肪分解

• 批准号: 6162079
• 负责人: R PRATLEY
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6162079
• 关键词: adipose tissue; adrenal medulla; basal metabolism; clinical research; exercise; hormone regulation /control mechanism; human subject; innervation; isoproterenol; lipolysis; microdialysis; spinal cord injury; sympathetic nervous system

The extent to which the sympathetic nervous system and other neuroendocrine transmitters regulate adipose tissue lipolysis during basal and stimulated conditions is unclear. Individuals with a spinal cord injury (SCI) lack direct sympathetic innervation to tissues below the level of lesion. This disruption of neural pathways affects both direct stimulation of abdominal fat tissues as well as indirect stimulation by hormones (i.e., catecholamines) of the adrenal medulla. Person's with SCI, therefore, represent a model for examining lipolysis in the absence of major sympathetic drive. This protocol examined the effects of exercise and isoproterenol stimulation on adipose tissue lipolysis in persons with and without sympathetic innervation of the adrenal medulla and adipose tissue. To test the role of direct sympathetic innervation and circulating catecholamines in regulating fat breakdown in adipose tissue, lipolysis (i.e., glycerol production was measured in situ by microdialysis in able-bodied subjects and in SCI persons at rest and during arm exercise. Seven patients with high SCI (C6-T5), 4 with low SCI (T10-L2) and 10 controls were compared. There were no group differences in basal rates of lipolysis (dialysate glycerol concentrations 30+/-5, 27+/-9 and 40+/-27 umol/l, respectively), isoproterenol stimulated lipolysis (79+/-28, 62+/-24, 93+/-87 umol/l) or during submaximal exercise (69+/-16, 75+/-40 and 97+/-70 umol/l). Plasma epinephrine and norepinephrine responses to submaximal exercise were significantly lower in SCI patients than controls. These results indicate that SCI patients maintain similar lipolytic rates and responses despite disruption of direct sympathetic innervation and lower circulating catecholamine levels. Compensatory mechanisms, such as down regulation of alpha, antilipolytic responses, may serve to maintain normal lipolytic rates in these individuals. This project is terminated as of the 1997 fiscal year.

交感神经系统和其他神经系统 神经内分泌递质调节脂肪组织脂解 基础和刺激条件尚不清楚。 患有脊柱 脊髓损伤（SCI）缺乏对下方组织的直接交感神经支配 病变程度。 这种神经通路的中断影响了 直接刺激腹部脂肪组织以及间接 激素刺激（即，肾上腺髓质的儿茶酚胺）。 因此，SCI患者代表了检查脂解的模型 在缺乏主要的交感神经驱动的情况下。 该方案检查了 运动和异丙肾上腺素刺激对脂肪组织的影响 有和没有交感神经支配的人的脂解 肾上腺髓质和脂肪组织。 为了测试直接 交感神经支配和循环儿茶酚胺在调节脂肪中的作用 脂肪组织的分解，脂解（即，甘油生产是 在身体健全的受试者和SCI患者中通过微透析原位测量 人在休息和手臂运动。 7例高度SCI患者 （C6-T5）、4例低SCI（T10-L2）和10例对照进行比较。 那里 基础脂解率（透析液）无组间差异 甘油浓度为30+/-5，27 +/-9和40+/-27 umol/l， 异丙肾上腺素刺激的脂解作用（79+/-28，62 +/-24， 93+/-87 umol/l）或次极量运动期间（69+/-16，75 +/-40和 97+/-70 umol/l）。 血浆肾上腺素和去甲肾上腺素对 亚极量运动在SCI患者中显著低于 对照 这些结果表明，SCI患者保持类似的 脂肪分解率和反应，尽管直接交感神经 神经支配和较低的循环儿茶酚胺水平。 补偿性 机制，如α的下调，抗脂肪分解反应， 可能有助于维持这些个体的正常脂解速率。 这 项目于1997财政年度结束。