FATTY ACID ABSORPTION AND METABOLISM IN PIMA INDIANS

皮马印第安人的脂肪酸吸收和代谢

• 批准号: 5202089
• 负责人: R PRATLEY
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/5202089
• 关键词: Native Americans; alanine; fatty acid binding protein; fatty acid metabolism; fatty acids; gastrointestinal nutrient absorption; human subject; oxidation; palmitates; protein sequence; threonine

The intestinal fatty acid binding protein (IFABP) is present in humans in two forms which differ by a alanine to threonine substitution at amino acid 54. In vitro, the threonine form (Thr54) is characterized by a two- fold higher binding affinity for long chain fatty acids than the alanine form (Ala54). The Thr54 form also is associated with higher plasma insulin concentrations and insulin resistance in the Pima Indians. This project examines whether the in vitro binding differences are physiologically significant. Net dietary fatty acid absorption, the kinetics of appearance of dietary fatty acids into plasma, and the time course of lipid oxidation will be determined following a standard test meal incorporating [l-13C]-palmitic acid as a tracer of fatty acid metabolism in healthy subjects homozygous for either the Thr54 form (n=20) or the Ala54 form of IFABP (n=20). These results will be related to rates of 24-hour substrate oxidation (measured in the respiratory chamber).

肠道脂肪酸结合蛋白(IFABP)存在于人类体内 在氨基上以丙氨酸取代苏氨酸而不同的两种形式 酸54。在体外，苏氨酸形式(Thr54)的特征是两个- 对长链脂肪酸的结合亲和力比丙氨酸高一倍 表格(Ala54)。Thr54也与较高的血浆浓度有关 皮马印第安人的胰岛素浓度和胰岛素抵抗。这 该项目检查了体外结合差异是否 生理上意义重大。膳食脂肪酸净吸收率 膳食脂肪酸进入血浆的动力学及时间 脂类氧化过程将在标准测试后确定 添加[L-13C]-棕榈酸作为脂肪酸示踪剂的膳食 Thr54纯合的健康受试者的代谢 或Ala54形式的IFABP(n=20)。这些结果将是相关的 至24小时底物氧化速率(在呼吸中测量 会议厅)。