DETERMINATION OF URUSHIOL IN ALLERGENIC EXTRACTS--URUSHIOL STABILITY

致敏提取物中漆酚的测定-漆酚稳定性

• 批准号: 2569083
• 负责人: A DEL GROSSO
• 金额: --
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2569083
• 关键词: allergens; analytical method; catechols; chemical reaction; chemical stability; drug quality /standard; gas chromatography; high performance liquid chromatography; method development; plant extracts; polymers; vegetable oils

The active constituents in allergenic extracts of Poison Ivy and other plants of the genus toxicodendron have been generically designated as "urushiol", a mixture of penta- or heptadecenyl catechols. Extracts for oral administration or injection are formulated in plant oils, e.g. almond, corn or olive oil or ethanol. Urushiol compounds may oxidize to orthoquinones which are capable of undergoing Michael-addition reactions with nucleophilic compounds. Olefinic sites on other urushiol molecules may function as nucleophiles, resulting in oligomerization of urushiols present in solution. Objectives of this project are: a) to develop methods for the quantitative analysis of urushiol, including speciation of individual compounds; b) to develop sample preparation techniques to isolate urushiol from plant oil matrices; c) to develop methodologies to determine oxidized and oligomerized urushiol degradation products; d) to correlate urushiol degradation with factors of concentration, solution type and storage conditions. Urushiol samples have been speciated and quantitated by gas chromatography of trimethylsilyl derivatives. Validation studies have been performed for this method. A normal phase HPLC method utilizing a silica column is to be investigated. Development of a capillary gas chromatographic procedure, providing better resolution of individual compounds is in progress. Procedures for the isolation of urushiol at high (greater than 100 ppm) concentrations from plant oils have been validated, development of a solid phase extraction procedure for isolation of urushiol at low (less than 10 ppm) concentrations is in progress. Initial studies involving speciation of urushiol oligomers by gel permeation chromatography have been performed.

毒葛等植物致敏提取物中的活性成分 毒木属植物一般被命名为 “漆酚”是五烯基或十七烯基儿茶酚的混合物。 提取物 口服给药或注射用植物油配制，例如， 杏仁、玉米或橄榄油或乙醇。 漆酚化合物可氧化成 能够进行迈克尔加成反应的邻醌 亲核化合物。 其他漆酚分子上的烯烃位点 可作为亲核试剂，导致漆酚的低聚 存在于溶液中。 该项目的目标是：（a）开发 漆酚的定量分析方法 B）开发样品制备技术， 从植物油基质中分离漆酚; c）开发方法， 测定氧化和低聚漆酚降解产物; d） 将漆酚降解与浓度、溶解度等因素进行关联 类型和储存条件。 漆酚样品已被物种化， 通过三甲基甲硅烷基衍生物的气相色谱定量。 已对该方法进行了验证研究。 正相 将研究使用硅胶柱的HPLC方法。 发展 毛细管气相色谱程序，提供更好的分辨率 个别化合物的研究正在进行中。 隔离的程序 来自植物油的高浓度（大于100 ppm）漆酚 已得到验证，开发了固相萃取程序 用于分离低浓度（小于10 ppm）的漆酚的方法是 中求进工作总 漆酚低聚物形态的初步研究 已经进行了凝胶渗透色谱法。