DETERMINATION OF URUSHIOL IN ALLERGENIC EXTRACTS--URUSHIOL STABILITY

致敏提取物中漆酚的测定-漆酚稳定性

• 批准号: 6101334
• 负责人: A DEL GROSSO
• 金额: --
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6101334
• 关键词: allergens; analytical method; catechols; chemical reaction; chemical stability; drug quality /standard; gas chromatography; high performance liquid chromatography; method development; plant extracts; plant poisons; polymers; vegetable oils

The active constituents in allergenic extracts of Poison Ivy and other plants of the genus toxicodendron have been generically designated as "urushiol", a mixture of penta-or heptadecenyl catechols. Objectives of this project are: a) to develop methods for the quantitative analysis of urushiol, including speciation of individual compounds; b) to develop sample preparation techniques to isolate urushiol from plant oil matrices; c) to develop methodologies to determine oxidized and oligomerized urushiol degradation products; d) to correlate urushiol degradation with factors of concentration, solution type and storage conditions. Urushiol samples have been speciated and quantitated by gas chromatography of trimethylsilyl derivatives. Validation studies have been performed for this method. A normal phase HPLC method utilizing a silica column is to be investigated. Development of a capillary gas chromatographic procedure, providing better resolution of individual compounds is in progress. Procedures for the isolation of urushiol at high (greater than 100 ppm) concentrations from plant oils have been validated.

毒藤等变应原提取物中的活性成分 毒柏属植物通常被指定为 “漆酚”，一种五或七十八烯基儿茶酚的混合物。目标 这个项目是：a)开发定量分析的方法 漆酚，包括个别化合物的形态形成；b)开发 从植物油基质中分离漆酚的样品制备技术； C)制定确定氧化态和寡聚态的方法 漆酚降解产物；d)漆酚降解与 浓度、溶液类型和储存条件的影响因素。漆酚 样品已经用气相色谱进行了形态分析和定量 三甲基硅基衍生物。已对以下各项进行了验证研究 这种方法。一种使用硅胶柱的正相高效液相色谱方法 被调查。毛细管气相色谱的研制 程序，提供更好的个别化合物的分辨率在 进步。高浓度漆酚的分离程序(大于 100ppm)的植物油浓度已得到验证。