DETERMINATION OF URUSHIOL IN ALLERGENIC EXTRACTS--URUSHIOL STABILITY

毒葛等致敏提取物中的活性成分 毒木属植物一般被命名为 “漆酚”是指十五烯基或十七烯基儿茶酚的混合物。 目标 这些项目是： （a）制定定量分析方法， 漆酚，包括单个化合物的形态; B）开发 从植物油基质中分离漆酚的样品制备技术; c）开发确定氧化和低聚 漆酚降解产物; d）将漆酚降解与 浓度、溶液类型和储存条件等因素。 漆酚 样品已通过气相色谱法进行了形态分析和定量， 三甲基甲硅烷基衍生物。 已进行了验证研究， 该方法 使用硅胶柱的正相HPLC方法是 追究 毛细管气相色谱仪的研制 程序，提供更好的分辨率的个别化合物是在 中求进工作总 在高（大于100 ℃）温度下分离漆酚的方法 100 ppm）的浓度。

The active constituents in allergenic extracts of Poison Ivy and other plants of the genus toxicodendron have been generically designated as "urushiol", a mixture of penta-or heptadecenyl catechols. Objectives of this project are: a) to develop methods for the quantitative analysis of urushiol, including speciation of individual compounds; b) to develop sample preparation techniques to isolate urushiol from plant oil matrices; c) to develop methodologies to determine oxidized and oligomerized urushiol degradation products; d) to correlate urushiol degradation with factors of concentration, solution type and storage conditions. Urushiol samples have been speciated and quantitated by gas chromatography of trimethylsilyl derivatives. Validation studies have been performed for this method. A normal phase HPLC method utilizing a silica column is to be investigated. Development of a capillary gas chromatographic procedure, providing better resolution of individual compounds is in progress. Procedures for the isolation of urushiol at high (greater than 100 ppm) concentrations from plant oils have been validated.