HIGH PERFORMANCE METAL CHELATE INTERACTION CHROMATOGRAPHY

一种可络合的二乙烯三胺功能固定相 开发了过渡金属离子，并对其在高效液相色谱中的使用进行了评估 分居。建立了一个经验保留率表达式，该公式与 色谱保留：a)酸性物质的静电相互作用 磺胺功能与阳离子固定相，b)二次 含杂环芳香化合物的金属-配体络合物 溶质的取代基和c)相对分子的增溶 流动相中的疏水溶质。保留率则相反 与有机改性剂和缓冲剂的浓度成正比，50% 甲醇有效地减少了静电相互作用，而 允许基于金属-配体相互作用的保留。以手稿为基础的 这项研究正在进行中。

A diethylenetriamine functional stationary phase, capable of complexing transition metal ions, was developed and evaluated for use in HPLC separations. An empirical retention expression was developed that related chromatographic retention to: a) electrostatic interaction of the acidic sulfonamide function with the cationic stationary phase, b) secondary metal-ligand complexations involving the heterocyclic aromatic substituents of the solutes and c) solubilization of the relatively hydrophobic solutes in the mobile phase. Retentions were inversely proportional to concentrations of organic modifier and buffer and 50% methanol was effective in minimizing electrostatic interactions while allowing retention based on metal-ligand interactions. A manuscript based on this study is in progress.