DETERMINATION OF URUSHIOL IN ALLERGENIC EXTRACTS--URUSHIOL STABILITY

致敏提取物中漆酚的测定-漆酚稳定性

• 批准号: 3748317
• 负责人: A DEL GROSSO
• 金额: --
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3748317
• 关键词: allergens; analytical method; catechols; chemical reaction; chemical stability; contact dermatitis; drug quality /standard; gas chromatography; high performance liquid chromatography; method development; oxidation; plant extracts; polymerization; polymers; vegetable oils

The active constituents in allergenic extracts of Poison Ivy and other plants of the genus toxicodendron have been generically designated as "urushiol", a mixture of penta- or heptadecenyl catechols. Extracts for oral administration or injection are formulated in plant oils, e.g. almond, corn or olive oil or ethanol. Urushiol compounds may oxidize to orthoquinones which are capable of undergoing Michael-addition reactions with nucleophilic compounds. Olefinic sites on other urushiol molecules may function as nucleophiles, resulting in oligomerization of urushiols present in solution. Objectives of this project are: a) to develop methods for the quantitative analysis of urushiol, including speciation of individual compounds; b) to develop sample preparation techniques to isolate urushiol from plant oil matrices; c) to develop methodologies to determine oxidized and oligomerized urushiol degradation products; d) to correlate urushiol degradation with factors of concentration, solution type and storage conditions. Urushiol samples have been speciated and quantitated by gas chromatography of trimethylsilyl derivatives. Validation studies have been performed for this method. A normal phase HPLC method utilizing a silica column is to be investigated. Development of a capillary gas chromatographic procedure, providing better resolution of individual compounds is in progress. Procedures for the isolation of urushiol at high (greater than 100 ppm) concentrations from plant oils have been validated, development of a solid phase extraction procedure for isolation of urushiol at low (less than 10 ppm) concentrations is in progress. Initial studies involving speciation of urushiol oligomers by gel permeation chromatography have been performed.

毒藤等变应原提取物中的活性成分 毒柏属植物通常被指定为 “漆酚”，一种五或七十八烯基儿茶酚的混合物。摘录 口服或注射用植物油，例如 杏仁、玉米、橄榄油或乙醇。漆酚化合物可能会被氧化成 能发生Michael-加成反应的邻苯二酚 含有亲核化合物。其他漆酚分子上的烯基 可能起到亲核的作用，导致漆酚的齐聚 存在于溶液中。该项目的目标是：a)开发 漆酚的定量分析方法，包括形态分析 单个化合物的分析；b)开发样品制备技术以 从植物油基质中分离漆酚；c)开发方法学以 测定氧化和齐聚漆酚降解产物；d) 漆酚降解与浓度、溶液等因素的相关性 类型和存储条件。漆酚样本已经形成形态， 用三甲基硅基衍生物的气相色谱进行定量。 对该方法进行了验证研究。正常阶段 使用硅胶色谱柱的高效液相色谱方法将被研究。发展 毛细管气相色谱程序，提供更好的分辨率 对个别化合物的研究正在进行中。隔离的程序 从植物油中提取高浓度(大于100ppm)的漆酚 已经得到验证，开发了一种固相萃取程序 用于分离低浓度(小于10ppm)的漆酚 进步。漆酚低聚物形态形成的初步研究 进行了凝胶渗透色谱分离。