NUTRIENT ANTIOXIDANT DEFENSE OF HIGH DENSITY LIPOPROTEIN

描述（改编自申请人摘要）：累积暴露于 活性氧物质被认为是 老年退行性疾病 假设氧化低 密度脂蛋白（LDL）是动脉粥样硬化的主要病因 心血管疾病导致了对LDL氧化的兴趣激增。 然而，即使高密度脂蛋白（HDL）在体内起着关键作用， 防止动脉粥样硬化，更容易氧化 比低密度脂蛋白，少得多的注意力已经针对影响， 氧化对HDL生物学特性的影响。 PI的试点数据表明， 饮食中的脂肪酸、抗氧化维生素和烟草的使用， 对HDL血浆浓度和氧化敏感性影响。 PI现在将进一步研究膳食脂肪酸的影响， 抗氧化维生素和烟草使用对血浆HDL浓度的影响， 低密度脂蛋白 在平行研究中，他将调查这些因素的影响， 高密度脂蛋白氧化的分子机制和营养素 抗氧化剂调节这些反应。 具体目标是：1）研究 膳食脂肪酸、维生素E和C以及吸烟对HDL的影响 水平在男性和女性受试者; 2）研究前和抗氧化剂 这些因素对HDL和LDL氧化反应的影响， 从饮食改良研究中分离的脂蛋白; 3）研究 使用重组HDL的HDL氧化和抗氧化作用的机制 具有明确定义的脂质、载脂蛋白和抗氧化剂的颗粒 混合物. 这项提案的长期目标是阐明特定的生物学 膳食促氧化剂和抗氧化剂对HDL氧化的影响。 这些知识 可能是重要的了解因素，调节高密度脂蛋白水平， 循环，并可能建议新的饮食方法，以预防 动脉粥样硬化性心血管疾病

DESCRIPTION (Adapted from the Applicant's Abstract): Cumulative exposure to reactive oxygen species has been proposed as a root cause of the degenerative diseases of aging. The hypothesis that oxidation of low density lipoproteins (LDL) is central to the etiology of atherosclerotic cardiovascular disease has led to an explosion of interest in LDL oxidation. However, even though high density lipoproteins (HDL) play a critical role in protecting against atherosclerosis and are more susceptible to oxidation than LDL, much less attention has been directed towards the effects of oxidation on the biological properties of HDL. The PI's pilot data suggest that dietary fatty acids, antioxidant vitamins and tobacco use have a major impact on HDL plasma concentration and oxidation susceptibility. The PI will now further examine the effects of dietary fatty acids, antioxidant vitamins and tobacco use on the plasma concentration of HDL and LDL. In parallel studies he will investigate the effects of these factors on the molecular mechanisms of HDL oxidation and the manner which nutrient antioxidants modulate these reactions. The specific aims are to: 1) study the effects of dietary fatty acids, vitamins E and C, and tobacco use on HDL levels in male and female subjects; 2) study the pro- and antioxidant effects of these factors on the oxidation reactions of HDL and LDL using lipoproteins isolated from the dietary modification studies; 3) study the mechanisms of HDL oxidation and antioxidant action using recombinant HDL particles with well-defined lipid, apolipoprotein and antioxidant composition. The long-term goal of this proposal is to elucidate the specific biologic effects of dietary pro- and antioxidants on HDL oxidation. This knowledge may be important in understanding the factors that regulate HDL levels in circulation and could suggest new dietary approaches to the prevention of atherosclerotic cardiovascular disease.