Studying early human kidney development using stem cells

利用干细胞研究人类早期肾脏发育

• 批准号: DP190101705
• 负责人: Prof Melissa Little
• 金额: $ 30.64万
• 依托单位: The University of Melbourne
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2019
• 资助国家: 澳大利亚
• 起止时间: 2019-06-14 至 2022-06-13
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP190101705
• 关键词: Studying; early; human; kidney; development

This project aims to improve our understanding of cell types, lineage relationships, cell-cell interactions and morphogenetic processes in human kidney development. Investigators have developed a method to produce multicellular kidney organoids from human pluripotent stem cells (hPSC). This project will use gene-edited reporter hPSC lines and high-res imaging to study the lineage relationships and morphogenetic mechanisms of these human kidney organoids. This project aims to validate the origin of nephrons in kidney organoids, study the origin of the renal stroma versus the nephron progenitor, and monitor nephron patterning and segmentation at a clonal level. This will improve our knowledge of human kidney development in a human model.

该项目旨在提高我们对人类肾脏发育中细胞类型、谱系关系、细胞间相互作用和形态发生过程的理解。研究人员已经开发出一种从人类多能干细胞（hPSC）生产多细胞肾类器官的方法。该项目将使用基因编辑的报告基因hPSC系和高分辨率成像来研究这些人类肾脏类器官的谱系关系和形态发生机制。该项目旨在验证肾类器官中肾单位的起源，研究肾基质与肾单位祖细胞的起源，并在克隆水平上监测肾单位的模式和分割。这将提高我们在人类模型中对人类肾脏发育的认识。