URINE ANTIPROLIFERATIVE PEPTIDE AND IC
尿液抗增殖肽和IC
基本信息
- 批准号:2770628
- 负责人:
- 金额:$ 17.04万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1997
- 资助国家:美国
- 起止时间:1997-09-30 至 2000-08-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
DESCRIPTION (Adapted from the Applicant's Abstract): Interstitial cystitis
(IC) is a chronic bladder disease with devastating effects on the lives of
approximately 450,000 people in the United States. The etiology of IC is
unknown, and there is currently no reliably effective treatment. It is
therefore important to continue to search for a cause of this debilitating
disorder, in order to systematically devise an effective therapy.
Preliminary data indicate the presence of a low molecular weight, heat
stable, factor(s) in the urine of IC patients that inhibits proliferation of
cultured normal adult human bladder epithelial cells. Because a damaged
bladder epithelium is a central finding in IC, it is possible that this
factor(s) contributes to the pathogenesis of this disease.
Substantial progress has been made in the purification and characterization
of this antiproliferative factor(s). Preliminary characterization including
trypsin treatment and isoelectric point determination indicates that this
factor is probably an acidic peptide. Based upon this information, a
sequential purification scheme has been devised that utilizes specific
chromatographic methods to obtain an approximately 50-fold increase in
specific activity of this peptide(s) with an active fraction that contains
only two peaks by UV spectrometry. Further purification and
characterization of this peptide(s) from additional IC patients is the first
priority, with attempts to determine whether it is excreted into the urine
or produced by cells from the upper or lower urinary tract. Whether the
peptide(s) is glycosylated and the general types of sugar moieties present
will also be assessed, and its antiproliferative activity confirmed by
blocking experiments using monospecific polyclonal rabbit antibodies.
The role of this peptide(s) in the pathogenesis of IC will be evaluated by
correlating the amount of the peptide(s) with cystometric findings and/or
symptom, severity in IC patients. Data from these studies should provide
valuable information regarding the possible role of a specific urine
peptide(s) in bladder epithelial cell proliferation and disease.
描述(改编自申请人摘要):间质性膀胱炎
(IC)是一种慢性膀胱疾病,
在美国大约有45万人。 IC的病因是
未知,目前没有可靠有效的治疗方法。 是
因此,重要的是继续寻找这种削弱的原因,
疾病,以便系统地设计有效的治疗方法。
初步数据显示存在一种低分子量,热
IC患者尿液中抑制细胞增殖的稳定因子
培养正常成人膀胱上皮细胞。 因为一个受损的
膀胱上皮是IC的中心发现,这可能是因为
这些因素促成了这种疾病的发病机制。
在纯化和表征方面取得了实质性进展
这种抗增殖因子。 初步特征包括
胰蛋白酶处理和等电点测定表明,
因子可能是一种酸性肽。 根据这些信息,A
已经设计了顺序纯化方案,其利用特定的
色谱方法,以获得约50倍的增加,
该肽的比活性与活性级分,所述活性级分含有
紫外光谱法只有两个峰。 进一步纯化和
来自额外IC患者的这种肽的表征是第一个
优先考虑,试图确定它是否被排泄到尿液中
或者由上尿路或下尿路的细胞产生。 是否
肽是糖基化的,并且存在糖部分的一般类型
也将进行评估,其抗增殖活性通过
使用单特异性多克隆兔抗体进行阻断实验。
将通过以下方法评价该肽在IC发病机制中的作用:
将肽的量与膀胱测压结果相关联,和/或
IC患者的症状、严重程度。 这些研究的数据应提供
关于特定尿液可能作用的宝贵信息
肽在膀胱上皮细胞增殖和疾病中的作用。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Susan F. Keay其他文献
Susan F. Keay的其他文献
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{{ truncateString('Susan F. Keay', 18)}}的其他基金
Abnormal Bladder Epithelial Cell Gene Expression in BPS/IC
BPS/IC 中膀胱上皮细胞基因表达异常
- 批准号:
8597942 - 财政年份:2012
- 资助金额:
$ 17.04万 - 项目类别:
Abnormal Bladder Epithelial Cell Gene Expression in BPS/IC
BPS/IC 中膀胱上皮细胞基因表达异常
- 批准号:
8965983 - 财政年份:2012
- 资助金额:
$ 17.04万 - 项目类别:
Abnormal Bladder Epithelial Cell Gene Expression in BPS/IC
BPS/IC 中膀胱上皮细胞基因表达异常
- 批准号:
8441105 - 财政年份:2012
- 资助金额:
$ 17.04万 - 项目类别:
Urine Antiproliferative Peptide in Interstitial Cystitis
间质性膀胱炎中的尿液抗增殖肽
- 批准号:
6517417 - 财政年份:1997
- 资助金额:
$ 17.04万 - 项目类别:
Urine Antiproliferative Peptide in Interstitial Cystitis
间质性膀胱炎中的尿液抗增殖肽
- 批准号:
6845147 - 财政年份:1997
- 资助金额:
$ 17.04万 - 项目类别:
Urine Antiproliferative Peptide in Interstitial Cystitis
间质性膀胱炎中的尿液抗增殖肽
- 批准号:
6635081 - 财政年份:1997
- 资助金额:
$ 17.04万 - 项目类别:
Urine Antiproliferative Peptide in Interstitial Cystitis
间质性膀胱炎中的尿液抗增殖肽
- 批准号:
7233286 - 财政年份:1997
- 资助金额:
$ 17.04万 - 项目类别:
Urine Antiproliferative Peptide in Interstitial Cystitis
间质性膀胱炎中的尿液抗增殖肽
- 批准号:
7596460 - 财政年份:1997
- 资助金额:
$ 17.04万 - 项目类别:
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