ENHANCED ROLLER BOTTLE FOR CELL CULTURE

用于细胞培养的增强型滚瓶

• 批准号: 2792660
• 负责人: MARTIN L WOLF
• 金额: $ 10.01万
• 依托单位: WILSON WOLF MANUFACTURING CORPORATION
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-02-01 至 2000-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2792660
• 关键词: biomedical equipment development; cell proliferation; cellular respiration; chondrocytes; consumable /disposable biomedical equipment; mass tissue /cell culture; material

An enhanced roller bottle concept is proposed. A roller bottle with an 8 fold or greater increase in bottle capacity will significantly aid operations in which multiple cultures are maintained. Importantly, tissue engineering is experiencing rapid growth. For many applications, one rate limiting step is availability of tissue and the need to expand small samples to clinically relevant endpoints. A compact, high capacity cell culture device with direct oxygenation (enhanced roller bottle) will be valuable. In autologous cultures, a compact, high capacity device that is modular, and efficient is needed. The device will be single use and simple to operate. Feasibility testing of the critical features required for such a device is to be done. The gas exchange/growth surface will be proven out. Multiple approaches will demonstrate robustness of the concept. The most suitable candidate will be incorporated into scale prototypes for device design prove out. Cell inoculation, capacity and recovery following culture will determine feasibility of the existing concept. Evaporative loss and medium foaming will be evaluated. If required, alternative designs will be tested. Final revised prototypes will be tested in the expansion of chondrocytes by an independent laboratory involved in a clinical trial using expanded chondrocytes. PROPOSED COMMERCIAL APPLICATION Annual use of roller bottles for cell culture is estimated to be between 25-40 x 106 bottles per year in the United States alone. The enhanced roller bottle is intended to be equivalent to at least 8 existing bottles with regards to the capacity of cells that can be supported. The enhanced roller bottle is envisioned to be an ideal device for tissue engineering as well as numerous other cell culture applications.

提出了一种改进的滚瓶概念。 瓶容量增加8倍或更多的滚瓶将显著有助于维持多种培养物的操作。 重要的是，组织工程正在经历快速增长。 对于许多应用，一个速率限制步骤是组织的可用性和将小样本扩展到临床相关终点的需要。 具有直接充氧的紧凑型高容量细胞培养装置（增强型滚瓶）将是有价值的。 在自体培养中，需要模块化且高效的紧凑、高容量装置。 该器械为一次性使用器械，操作简单。 将对此类设备所需的关键功能进行可行性测试。 气体交换/生长表面将被证明。多种方法将证明这一概念的稳健性。最合适的候选人将被纳入规模原型的设备设计证明了。 细胞接种、容量和培养后的恢复将决定现有概念的可行性。 将评估蒸发损失和介质发泡。 如果需要，将测试替代设计。 最终修订的原型将由参与使用扩增软骨细胞的临床试验的独立实验室在软骨细胞扩增中进行测试。仅在美国，用于细胞培养的滚瓶的年使用量估计在25-40 x 106瓶之间。就可支持的细胞容量而言，增强型滚瓶旨在等同于至少8个现有瓶。 增强型滚瓶预计将成为组织工程以及许多其他细胞培养应用的理想装置。